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Caveats of Reporting Cycle Threshold Values from Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Qualitative Polymerase Chain Reaction Assays: A Molecular Diagnostic Laboratory Perspective.

Clinical infectious diseases : an official publication of the Infectious Diseases Society of America

Poon KS, Wen-Sim Tee N.
PMID: 32929464
Clin Infect Dis. 2021 Nov 02;73(9):e2851-e2852. doi: 10.1093/cid/ciaa1399.

No abstract available.

Cite
Poon KS, Wen-Sim Tee N. Caveats of Reporting Cycle Threshold Values from Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Qualitative Polymerase Chain Reaction Assays: A Molecular Diagnostic Laboratory Perspective. Clin Infect Dis. 2021;73(9):e2851-e2852doi: 10.1093/cid/ciaa1399.
Poon, K. S., & Wen-Sim Tee, N. (2021). Caveats of Reporting Cycle Threshold Values from Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Qualitative Polymerase Chain Reaction Assays: A Molecular Diagnostic Laboratory Perspective. Clinical infectious diseases : an official publication of the Infectious Diseases Society of America, 73(9), e2851-e2852. https://doi.org/10.1093/cid/ciaa1399
Poon, Kok-Siong, and Wen-Sim Tee, Nancy. "Caveats of Reporting Cycle Threshold Values from Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Qualitative Polymerase Chain Reaction Assays: A Molecular Diagnostic Laboratory Perspective." Clinical infectious diseases : an official publication of the Infectious Diseases Society of America vol. 73,9 (2021): e2851-e2852. doi: https://doi.org/10.1093/cid/ciaa1399
Poon KS, Wen-Sim Tee N. Caveats of Reporting Cycle Threshold Values from Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Qualitative Polymerase Chain Reaction Assays: A Molecular Diagnostic Laboratory Perspective. Clin Infect Dis. 2021 Nov 02;73(9):e2851-e2852. doi: 10.1093/cid/ciaa1399. PMID: 32929464; PMCID: PMC7543341.
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miRNAs are stable in colorectal cancer archival tissue blocks.

Frontiers in bioscience (Elite edition)

Bovell L, Shanmugam C, Katkoori VR, Zhang B, Vogtmann E, Grizzle WE, Manne U.
PMID: 22202009
Front Biosci (Elite Ed). 2012 Jan 01;4:1937-40. doi: 10.2741/514.

MicroRNAs (miRNAs) have prognostic and therapeutic value for colorectal cancers RCs). Although formalin-fixed paraffin-embedded (FFPE) tissues are available for biomarker studies, the stability of miRNAs in these tissues stored for long periods (more than 20 years) is unknown. The...

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Bovell L, Shanmugam C, Katkoori VR, et al. miRNAs are stable in colorectal cancer archival tissue blocks. Front Biosci (Elite Ed). 2012;4:1937-40doi: 10.2741/514.
Bovell, L., Shanmugam, C., Katkoori, V. R., Zhang, B., Vogtmann, E., Grizzle, W. E., & Manne, U. (2012). miRNAs are stable in colorectal cancer archival tissue blocks. Frontiers in bioscience (Elite edition), 41937-40. https://doi.org/10.2741/514
Bovell, Liselle, et al. "miRNAs are stable in colorectal cancer archival tissue blocks." Frontiers in bioscience (Elite edition) vol. 4 (2012): 1937-40. doi: https://doi.org/10.2741/514
Bovell L, Shanmugam C, Katkoori VR, Zhang B, Vogtmann E, Grizzle WE, Manne U. miRNAs are stable in colorectal cancer archival tissue blocks. Front Biosci (Elite Ed). 2012 Jan 01;4:1937-40. doi: 10.2741/514. PMID: 22202009; PMCID: PMC3681606.
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MicroRNA detection in prostate tumors by quantitative real-time PCR (qPCR).

Journal of visualized experiments : JoVE

Gordanpour A, Nam RK, Sugar L, Bacopulos S, Seth A.
PMID: 22643910
J Vis Exp. 2012 May 16;(63):e3874. doi: 10.3791/3874.

MicroRNAs (miRNAs) are single-stranded, 18-24 nucleotide long, non-coding RNA molecules. They are involved in virtually every cellular process including development, apoptosis, and cell cycle regulation. MiRNAs are estimated to regulate the expression of 30% to 90% of human genes...

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Gordanpour A, Nam RK, Sugar L, et al. MicroRNA detection in prostate tumors by quantitative real-time PCR (qPCR). J Vis Exp. 2012;(63):e3874doi: 10.3791/3874.
Gordanpour, A., Nam, R. K., Sugar, L., Bacopulos, S., & Seth, A. (2012). MicroRNA detection in prostate tumors by quantitative real-time PCR (qPCR). Journal of visualized experiments : JoVE, (63), e3874. https://doi.org/10.3791/3874
Gordanpour, Aida, et al. "MicroRNA detection in prostate tumors by quantitative real-time PCR (qPCR)." Journal of visualized experiments : JoVE vol. ,63 (2012): e3874. doi: https://doi.org/10.3791/3874
Gordanpour A, Nam RK, Sugar L, Bacopulos S, Seth A. MicroRNA detection in prostate tumors by quantitative real-time PCR (qPCR). J Vis Exp. 2012 May 16;(63):e3874. doi: 10.3791/3874. PMID: 22643910; PMCID: PMC3466958.
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Enhancer chip: detecting human copy number variations in regulatory elements.

PloS one

Savarese M, Piluso G, Orteschi D, Di Fruscio G, Dionisi M, Blanco Fdel V, Torella A, Giugliano T, Iacomino M, Zollino M, Neri G, Nigro V.
PMID: 23284961
PLoS One. 2012;7(12):e52264. doi: 10.1371/journal.pone.0052264. Epub 2012 Dec 20.

Critical functional properties are embedded in the non-coding portion of the human genome. Recent successful studies have shown that variations in distant-acting gene enhancer sequences can contribute to disease. In fact, various disorders, such as thalassaemias, preaxial polydactyly or...

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Savarese M, Piluso G, Orteschi D, et al. Enhancer chip: detecting human copy number variations in regulatory elements. PLoS One. 2012;7(12):e52264doi: 10.1371/journal.pone.0052264.
Savarese, M., Piluso, G., Orteschi, D., Di Fruscio, G., Dionisi, M., Blanco, F. d. e. l. . V., Torella, A., Giugliano, T., Iacomino, M., Zollino, M., Neri, G., & Nigro, V. (2012). Enhancer chip: detecting human copy number variations in regulatory elements. PloS one, 7(12), e52264. https://doi.org/10.1371/journal.pone.0052264
Savarese, Marco, et al. "Enhancer chip: detecting human copy number variations in regulatory elements." PloS one vol. 7,12 (2012): e52264. doi: https://doi.org/10.1371/journal.pone.0052264
Savarese M, Piluso G, Orteschi D, Di Fruscio G, Dionisi M, Blanco Fdel V, Torella A, Giugliano T, Iacomino M, Zollino M, Neri G, Nigro V. Enhancer chip: detecting human copy number variations in regulatory elements. PLoS One. 2012;7(12):e52264. doi: 10.1371/journal.pone.0052264. Epub 2012 Dec 20. PMID: 23284961; PMCID: PMC3527541.
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Single-Cell Quantitative PCR: Advances and Potential in Cancer Diagnostics.

Methods in molecular biology (Clifton, N.J.)

Ok CY, Singh RR, Salim AA.
PMID: 26843054
Methods Mol Biol. 2016;1392:153-9. doi: 10.1007/978-1-4939-3360-0_14.

Tissues are heterogeneous in their components. If cells of interest are a minor population of collected tissue, it would be difficult to obtain genetic or genomic information of the interested cell population with conventional genomic DNA extraction from the...

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Ok CY, Singh RR, Salim AA. Single-Cell Quantitative PCR: Advances and Potential in Cancer Diagnostics. Methods Mol Biol. 2016;1392:153-9doi: 10.1007/978-1-4939-3360-0_14.
Ok, C. Y., Singh, R. R., & Salim, A. A. (2016). Single-Cell Quantitative PCR: Advances and Potential in Cancer Diagnostics. Methods in molecular biology (Clifton, N.J.), 1392153-9. https://doi.org/10.1007/978-1-4939-3360-0_14
Ok, Chi Young, et al. "Single-Cell Quantitative PCR: Advances and Potential in Cancer Diagnostics." Methods in molecular biology (Clifton, N.J.) vol. 1392 (2016): 153-9. doi: https://doi.org/10.1007/978-1-4939-3360-0_14
Ok CY, Singh RR, Salim AA. Single-Cell Quantitative PCR: Advances and Potential in Cancer Diagnostics. Methods Mol Biol. 2016;1392:153-9. doi: 10.1007/978-1-4939-3360-0_14. PMID: 26843054.
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The state of RT-quantitative PCR: firsthand observations of implementation of minimum information for the publication of quantitative real-time PCR experiments (MIQE).

Journal of molecular microbiology and biotechnology

Taylor SC, Mrkusich EM.
PMID: 24296827
J Mol Microbiol Biotechnol. 2014;24(1):46-52. doi: 10.1159/000356189. Epub 2013 Nov 28.

In the past decade, the techniques of quantitative PCR (qPCR) and reverse transcription (RT)-qPCR have become accessible to virtually all research labs, producing valuable data for peer-reviewed publications and supporting exciting research conclusions. However, the experimental design and validation...

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Taylor SC, Mrkusich EM. The state of RT-quantitative PCR: firsthand observations of implementation of minimum information for the publication of quantitative real-time PCR experiments (MIQE). J Mol Microbiol Biotechnol. 2013;24(1):46-52doi: 10.1159/000356189.
Taylor, S. C., & Mrkusich, E. M. (2014). The state of RT-quantitative PCR: firsthand observations of implementation of minimum information for the publication of quantitative real-time PCR experiments (MIQE). Journal of molecular microbiology and biotechnology, 24(1), 46-52. https://doi.org/10.1159/000356189
Taylor, Sean C, and Mrkusich, Eli M. "The state of RT-quantitative PCR: firsthand observations of implementation of minimum information for the publication of quantitative real-time PCR experiments (MIQE)." Journal of molecular microbiology and biotechnology vol. 24,1 (2014): 46-52. doi: https://doi.org/10.1159/000356189
Taylor SC, Mrkusich EM. The state of RT-quantitative PCR: firsthand observations of implementation of minimum information for the publication of quantitative real-time PCR experiments (MIQE). J Mol Microbiol Biotechnol. 2014;24(1):46-52. doi: 10.1159/000356189. Epub 2013 Nov 28. PMID: 24296827.
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MicroRNA Analysis Using the Quantitative Real-Time PCR Reaction.

Methods in molecular biology (Clifton, N.J.)

Kotlarek M, Kubiak A, Jażdżewski K, Wójcicka A.
PMID: 29959675
Methods Mol Biol. 2018;1823:69-85. doi: 10.1007/978-1-4939-8624-8_7.

The analysis of microRNA expression patterns provides new insights into numerous cellular processes and their aberrances in diseases. Despite its potential pitfalls, the quantitative real-time polymerase chain reaction (qPCR) is the most commonly used tool for microRNA profiling. The...

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Kotlarek M, Kubiak A, Jażdżewski K, et al. MicroRNA Analysis Using the Quantitative Real-Time PCR Reaction. Methods Mol Biol. 2018;1823:69-85doi: 10.1007/978-1-4939-8624-8_7.
Kotlarek, M., Kubiak, A., Jażdżewski, K., & Wójcicka, A. (2018). MicroRNA Analysis Using the Quantitative Real-Time PCR Reaction. Methods in molecular biology (Clifton, N.J.), 182369-85. https://doi.org/10.1007/978-1-4939-8624-8_7
Kotlarek, Marta, et al. "MicroRNA Analysis Using the Quantitative Real-Time PCR Reaction." Methods in molecular biology (Clifton, N.J.) vol. 1823 (2018): 69-85. doi: https://doi.org/10.1007/978-1-4939-8624-8_7
Kotlarek M, Kubiak A, Jażdżewski K, Wójcicka A. MicroRNA Analysis Using the Quantitative Real-Time PCR Reaction. Methods Mol Biol. 2018;1823:69-85. doi: 10.1007/978-1-4939-8624-8_7. PMID: 29959675.
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Extreme PCR Meets High-Speed Melting: A Step Closer to Molecular Diagnostics "While You Wait".

Clinical chemistry

Makrigiorgos GM.
PMID: 30530717
Clin Chem. 2019 Feb;65(2):217-219. doi: 10.1373/clinchem.2018.298356. Epub 2018 Dec 10.

No abstract available.

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Makrigiorgos GM. Extreme PCR Meets High-Speed Melting: A Step Closer to Molecular Diagnostics "While You Wait". Clin Chem. 2018;65(2):217-219doi: 10.1373/clinchem.2018.298356.
Makrigiorgos, G. M. (2019). Extreme PCR Meets High-Speed Melting: A Step Closer to Molecular Diagnostics "While You Wait". Clinical chemistry, 65(2), 217-219. https://doi.org/10.1373/clinchem.2018.298356
Makrigiorgos, G Mike. "Extreme PCR Meets High-Speed Melting: A Step Closer to Molecular Diagnostics "While You Wait"." Clinical chemistry vol. 65,2 (2019): 217-219. doi: https://doi.org/10.1373/clinchem.2018.298356
Makrigiorgos GM. Extreme PCR Meets High-Speed Melting: A Step Closer to Molecular Diagnostics "While You Wait". Clin Chem. 2019 Feb;65(2):217-219. doi: 10.1373/clinchem.2018.298356. Epub 2018 Dec 10. PMID: 30530717; PMCID: PMC7101026.
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Autoregressive modeling and diagnostics for qPCR amplification.

Bioinformatics (Oxford, England)

Hsu B, Sherina V, McCall MN.
PMID: 33244594
Bioinformatics. 2021 Apr 01;36(22):5386-5391. doi: 10.1093/bioinformatics/btaa1000.

MOTIVATION: Current methods used to analyze real-time quantitative polymerase chain reaction (qPCR) data exhibit systematic deviations from the assumed model over the progression of the reaction. Slight variations in the amount of the initial target molecule or in early...

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Hsu B, Sherina V, McCall MN. Autoregressive modeling and diagnostics for qPCR amplification. Bioinformatics. 2021;36(22):5386-5391doi: 10.1093/bioinformatics/btaa1000.
Hsu, B., Sherina, V., & McCall, M. N. (2021). Autoregressive modeling and diagnostics for qPCR amplification. Bioinformatics (Oxford, England), 36(22), 5386-5391. https://doi.org/10.1093/bioinformatics/btaa1000
Hsu, Benjamin, et al. "Autoregressive modeling and diagnostics for qPCR amplification." Bioinformatics (Oxford, England) vol. 36,22 (2021): 5386-5391. doi: https://doi.org/10.1093/bioinformatics/btaa1000
Hsu B, Sherina V, McCall MN. Autoregressive modeling and diagnostics for qPCR amplification. Bioinformatics. 2021 Apr 01;36(22):5386-5391. doi: 10.1093/bioinformatics/btaa1000. PMID: 33244594; PMCID: PMC8016497.
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Increased sensitivity using real-time dPCR for detection of SARS-CoV-2.

BioTechniques

Duong K, Ou J, Li Z, Lv Z, Dong H, Hu T, Zhang Y, Hanna A, Gordon S, Crynen G, Head SR, Ordoukhanian P, Wang Y.
PMID: 33222514
Biotechniques. 2021 Jan;70(1):7-20. doi: 10.2144/btn-2020-0133. Epub 2020 Nov 23.

A real-time dPCR system was developed to improve the sensitivity, specificity and quantification accuracy of end point dPCR. We compared three technologies - real-time qPCR, end point dPCR and real-time dPCR - in the context of SARS-CoV-2. Some improvement...

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Duong K, Ou J, Li Z, et al. Increased sensitivity using real-time dPCR for detection of SARS-CoV-2. Biotechniques. 2020;70(1):7-20doi: 10.2144/btn-2020-0133.
Duong, K., Ou, J., Li, Z., Lv, Z., Dong, H., Hu, T., Zhang, Y., Hanna, A., Gordon, S., Crynen, G., Head, S. R., Ordoukhanian, P., & Wang, Y. (2021). Increased sensitivity using real-time dPCR for detection of SARS-CoV-2. BioTechniques, 70(1), 7-20. https://doi.org/10.2144/btn-2020-0133
Duong, Kyra, et al. "Increased sensitivity using real-time dPCR for detection of SARS-CoV-2." BioTechniques vol. 70,1 (2021): 7-20. doi: https://doi.org/10.2144/btn-2020-0133
Duong K, Ou J, Li Z, Lv Z, Dong H, Hu T, Zhang Y, Hanna A, Gordon S, Crynen G, Head SR, Ordoukhanian P, Wang Y. Increased sensitivity using real-time dPCR for detection of SARS-CoV-2. Biotechniques. 2021 Jan;70(1):7-20. doi: 10.2144/btn-2020-0133. Epub 2020 Nov 23. PMID: 33222514; PMCID: PMC7888512.
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Pre-Operative SARS CoV-2 Rapid Antigen Test and Reverse Transcription Polymerase Chain Reaction: A conundrum in surgical decision making.

Indian journal of ophthalmology

Kumar KK, Sampritha UC, Maganty V, Prakash AA, Basumatary J, Adappa K, Chandraprabha S, Neeraja TG, Guru Prasad NS, Preethi B, Gangasagara SB, Sujatha Rathod BL.
PMID: 34011741
Indian J Ophthalmol. 2021 Jun;69(6):1560-1562. doi: 10.4103/ijo.IJO_430_21.

PURPOSE: Since the eye is in close proximity to the oro-nasal cavity, transmission of SARS CoV-2 is higher during ophthalmic surgical procedures, it is vital to ensure the safety of the healthcare team by pre-operative Rapid Antigen Test (RAT)...

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Kumar KK, Sampritha UC, Maganty V, et al. Pre-Operative SARS CoV-2 Rapid Antigen Test and Reverse Transcription Polymerase Chain Reaction: A conundrum in surgical decision making. Indian J Ophthalmol. 2021;69(6):1560-1562doi: 10.4103/ijo.IJO_430_21.
Kumar, K. K., Sampritha, U. C., Maganty, V., Prakash, A. A., Basumatary, J., Adappa, K., Chandraprabha, S., Neeraja, T. G., Guru Prasad, N. S., Preethi, B., Gangasagara, S. B., & Sujatha Rathod, B. L. (2021). Pre-Operative SARS CoV-2 Rapid Antigen Test and Reverse Transcription Polymerase Chain Reaction: A conundrum in surgical decision making. Indian journal of ophthalmology, 69(6), 1560-1562. https://doi.org/10.4103/ijo.IJO_430_21
Kumar, K Kiran, et al. "Pre-Operative SARS CoV-2 Rapid Antigen Test and Reverse Transcription Polymerase Chain Reaction: A conundrum in surgical decision making." Indian journal of ophthalmology vol. 69,6 (2021): 1560-1562. doi: https://doi.org/10.4103/ijo.IJO_430_21
Kumar KK, Sampritha UC, Maganty V, Prakash AA, Basumatary J, Adappa K, Chandraprabha S, Neeraja TG, Guru Prasad NS, Preethi B, Gangasagara SB, Sujatha Rathod BL. Pre-Operative SARS CoV-2 Rapid Antigen Test and Reverse Transcription Polymerase Chain Reaction: A conundrum in surgical decision making. Indian J Ophthalmol. 2021 Jun;69(6):1560-1562. doi: 10.4103/ijo.IJO_430_21. PMID: 34011741; PMCID: PMC8302313.
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Diagnosis CNS Viral Infections by Real-Time PCR: Further Exploration Needed.

Neurology India

Garg RK.
PMID: 33342863
Neurol India. 2020 Nov-Dec;68(6):1331-1332. doi: 10.4103/0028-3886.304086.

No abstract available.

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Garg RK. Diagnosis CNS Viral Infections by Real-Time PCR: Further Exploration Needed. Neurol India. 2020;68(6):1331-1332doi: 10.4103/0028-3886.304086.
Garg, R. K. (2020). Diagnosis CNS Viral Infections by Real-Time PCR: Further Exploration Needed. Neurology India, 68(6), 1331-1332. https://doi.org/10.4103/0028-3886.304086
Garg, Ravindra Kumar. "Diagnosis CNS Viral Infections by Real-Time PCR: Further Exploration Needed." Neurology India vol. 68,6 (2020): 1331-1332. doi: https://doi.org/10.4103/0028-3886.304086
Garg RK. Diagnosis CNS Viral Infections by Real-Time PCR: Further Exploration Needed. Neurol India. 2020 Nov-Dec;68(6):1331-1332. doi: 10.4103/0028-3886.304086. PMID: 33342863.
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Showing 13 to 24 of 71 entries