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Showing 1 to 12 of 169 entries
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The Convenience of Single Homology Arm Donor DNA and CRISPR/Cas9-Nickase for Targeted Insertion of Long DNA Fragment.

Cell journal

Basiri M, Behmanesh M, Tahamtani Y, Khalooghi K, Moradmand A, Baharvand H.
PMID: 28042537
Cell J. 2017;18(4):532-539. doi: 10.22074/cellj.2016.4719. Epub 2016 Sep 26.

OBJECTIVE: CRISPR/Cas9 technology provides a powerful tool for targeted modification of genomes. In this system, a donor DNA harboring two flanking homology arms is mostly used for targeted insertion of long exogenous DNA. Here, we introduced an alternative design...

Evaluating Electroporation and Lipofectamine Approaches for Transient and Stable Transgene Expressions in Human Fibroblasts and Embryonic Stem Cells.

Cell journal

Sharifi Tabar M, Hesaraki M, Esfandiari F, Sahraneshin Samani F, Vakilian H, Baharvand H.
PMID: 26464815
Cell J. 2015;17(3):438-50. doi: 10.22074/cellj.2015.5. Epub 2015 Oct 07.

OBJECTIVE: Genetic modification of human embryonic stem cells (hESCs) is critical for their extensive use as a fundamental tool for cell therapy and basic research. Despite the fact that various methods such as lipofection and electroporation have been applied...

Amelioration of altered serum, liver, and kidney antioxidant enzymes activities by sodium selenite in alloxan-induced diabetic rats.

Reports of biochemistry & molecular biology

Ahmadvand H, Ghasemi Dehnoo M, Cheraghi R, Rasoulian B, Ezatpour B, Azadpour M, Baharvand K.
PMID: 26989732
Rep Biochem Mol Biol. 2014 Oct;3(1):14-20.

BACKGROUND: The aim of this study was to evaluate the possible protective effect of sodium selenite on serum, liver, and kidney antioxidant enzymes activities in alloxan-induced type 1 diabetic rats.METHODS: Forty Sprague-Dawley male rats were randomly divided into four...

Diagnosis of simulated condylar bone defects using panoramic radiography, spiral tomography and cone-beam computed tomography: A comparison study.

Journal of clinical and experimental dentistry

Salemi F, Shokri A, Mortazavi H, Baharvand M.
PMID: 25810839
J Clin Exp Dent. 2015 Feb 01;7(1):e34-9. doi: 10.4317/jced.51736. eCollection 2015 Feb.

OBJECTIVES: Radiographic examination is one of the most important parts of the clinical assessment routine for temporomandibular disorders. The aim of this study was to compare the diagnostic accuracy of cone-beam computed tomography(CBCT) with panoramic radiography and spiral computed...

Amplification of GC-rich Putative Mouse PeP Promoter using Betaine and DMSO in Ammonium Sulfate Polymerase Chain Reaction Buffer.

Avicenna journal of medical biotechnology

Seifi T, Ghaedi K, Salamian A, Tanhaei S, Safari F, Hojati Z, Tavassoli M, Baharvand H, Esfahani MH.
PMID: 23408119
Avicenna J Med Biotechnol. 2012 Oct;4(4):206-9.

BACKGROUND: Recently, we have shown that peroxisomal protein expression was induced upon retinoic acid treatment in mouse embryonic stem cells during the process of neurogenesis. Thus, characterization of the respective promoter could elucidate the molecular aspects of transcriptional regulation...

Xerostomia due to systemic disease: a review of 20 conditions and mechanisms.

Annals of medical and health sciences research

Mortazavi H, Baharvand M, Movahhedian A, Mohammadi M, Khodadoustan A.
PMID: 25221694
Ann Med Health Sci Res. 2014 Jul;4(4):503-10. doi: 10.4103/2141-9248.139284.

Xerostomia is a common complaint of nearly half of the elderly population and about one-fifth of younger adults. It causes several signs and symptoms, and compromise oral functions and health-related quality-of-life. Multiple reasons are proposed to describe the etiology...

Position of impacted mandibular third molar in different skeletal facial types: First radiographic evaluation in a group of Iranian patients.

Imaging science in dentistry

Shokri A, Mahmoudzadeh M, Baharvand M, Mortazavi H, Faradmal J, Khajeh S, Yousefi F, Noruzi-Gangachin M.
PMID: 24701460
Imaging Sci Dent. 2014 Mar;44(1):61-5. doi: 10.5624/isd.2014.44.1.61. Epub 2014 Mar 19.

PURPOSE: This study was performed to evaluate the position of impacted mandibular third molars in different skeletal facial types among a group of Iranian patients.MATERIALS AND METHODS: A total of 400 mandibular third molars in 200 subjects with different...

Correction to: Efficient Differentiation of Human Embryonic Stem Cells Toward Dopaminergic Neurons Using Recombinant LMX1A Factor.

Molecular biotechnology

Fathi A, Rasouli H, Yeganeh M, Salekdeh GH, Baharvand H.
PMID: 30390207
Mol Biotechnol. 2019 Jan;61(1):72. doi: 10.1007/s12033-018-0131-2.

The original version of this article was published without article note. The article note is given below.

Draft genome of .

Zoological letters

An Y, Kawaguchi A, Zhao C, Toyoda A, Sharifi-Zarchi A, Mousavi SA, Bagherzadeh R, Inoue T, Ogino H, Fujiyama A, Chitsaz H, Baharvand H, Agata K.
PMID: 30181897
Zoological Lett. 2018 Aug 29;4:24. doi: 10.1186/s40851-018-0102-2. eCollection 2018.

BACKGROUND: Planarians are non-parasitic Platyhelminthes (flatworms) famous for their regeneration ability and for having a well-organized brain. RESULTS: To obtain whole-genome information on this species and to study its gene regulatory networks, we extracted genomic DNA from 200 planarians...

Establishment of A Protocol for In Vitro Culture of Cardiogenic Mesodermal Cells Derived from Human Embryonic Stem Cells.

Cell journal

Vahdat S, Pahlavan S, Aghdami N, Bakhshandeh B, Baharvand H.
PMID: 30123995
Cell J. 2019 Jan;20(4):496-504. doi: 10.22074/cellj.2019.5661. Epub 2018 Dec 12.

OBJECTIVE: Cardiovascular progenitor cells (CPCs) are introduced as one of the promising cell sources for preclinical studies and regenerative medicine. One of the earliest type of CPCs is cardiogenic mesoderm cells (CMCs), which have the capability to generate all...

Establishing a new animal model for muscle regeneration studies.

Molecular biology research communications

Pourghadamyari H, Rezaei M, Ipakchi-Azimi A, Eisa-Beygi S, Basiri M, Tahamtani Y, Baharvand H.
PMID: 32042835
Mol Biol Res Commun. 2019 Dec;8(4):171-179. doi: 10.22099/mbrc.2019.34611.1433.

Skeletal muscle injuries are one of the most common problems in the worldwide which impose a substantial financial burden to the health care system. Accordingly, it widely accepted that muscle regeneration is a promising approach that can be used...

SOX2 protein transduction directly converts human fibroblasts into oligodendrocyte-like cells.

Biochemical and biophysical research communications

Pouya A, Rassouli H, Rezaei-Larijani M, Salekdeh GH, Baharvand H.
PMID: 32070492
Biochem Biophys Res Commun. 2020 Feb 15; doi: 10.1016/j.bbrc.2020.02.047. Epub 2020 Feb 15.

Oligodendrocyte precursor cells (OPCs) are ideal therapeutic cells for treatment of spinal cord injuries and diseases that affect myelin. However, it is necessary to generate a cell population with a low risk of teratoma formation and oncogenesis from a...

Showing 1 to 12 of 169 entries