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Using PAT to accelerate the transition to continuous API manufacturing.

Analytical and bioanalytical chemistry

Gouveia FF, Rahbek JP, Mortensen AR, Pedersen MT, Felizardo PM, Bro R, Mealy MJ.
PMID: 27515798
Anal Bioanal Chem. 2017 Jan;409(3):821-832. doi: 10.1007/s00216-016-9834-z. Epub 2016 Aug 11.

Significant improvements can be realized by converting conventional batch processes into continuous ones. The main drivers include reduction of cost and waste, increased safety, and simpler scale-up and tech transfer activities. Re-designing the process layout offers the opportunity to...

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Gouveia FF, Rahbek JP, Mortensen AR, et al. Using PAT to accelerate the transition to continuous API manufacturing. Anal Bioanal Chem. 2016;409(3):821-832doi: 10.1007/s00216-016-9834-z.
Gouveia, F. F., Rahbek, J. P., Mortensen, A. R., Pedersen, M. T., Felizardo, P. M., Bro, R., & Mealy, M. J. (2017). Using PAT to accelerate the transition to continuous API manufacturing. Analytical and bioanalytical chemistry, 409(3), 821-832. https://doi.org/10.1007/s00216-016-9834-z
Gouveia, Francisca F, et al. "Using PAT to accelerate the transition to continuous API manufacturing." Analytical and bioanalytical chemistry vol. 409,3 (2017): 821-832. doi: https://doi.org/10.1007/s00216-016-9834-z
Gouveia FF, Rahbek JP, Mortensen AR, Pedersen MT, Felizardo PM, Bro R, Mealy MJ. Using PAT to accelerate the transition to continuous API manufacturing. Anal Bioanal Chem. 2017 Jan;409(3):821-832. doi: 10.1007/s00216-016-9834-z. Epub 2016 Aug 11. PMID: 27515798.
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[Surgery of pulmonary metastasis].

Revista portuguesa de cirurgia cardio-toracica e vascular : orgao oficial da Sociedade Portuguesa de Cirurgia Cardio-Toracica e Vascular

Cruz J, Felizardo M, Silva J, Monteiro F, Rodrigues T, Caldeira J, Costa L, Cravino J.
PMID: 18618046
Rev Port Cir Cardiotorac Vasc. 2008 Jan-Mar;15(1):15-7.

UNLABELLED: Between April 1993 and December 2007, 92 patients underwent the complete resection of pulmonary metastases, 47 mens (51.1%) and 45 womens (49.9%). 26 patients (27.2%) had solitary pulmonary metastasis, 25 (26.1%) multiple pulmonary metastasis and 41 (44.5%) patients...

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Cruz J, Felizardo M, Silva J, et al. Cirurgia das metáses pulmonares. [Surgery of pulmonary metastasis]. Rev Port Cir Cardiotorac Vasc. 2008;15(1):15-7
Cruz, J., Felizardo, M., Silva, J., Monteiro, F., Rodrigues, T., Caldeira, J., Costa, L., & Cravino, J. (2008). Cirurgia das metáses pulmonares. [Surgery of pulmonary metastasis]. Revista portuguesa de cirurgia cardio-toracica e vascular : orgao oficial da Sociedade Portuguesa de Cirurgia Cardio-Toracica e Vascular, 15(1), 15-7.
Cruz, Jorge, et al. "Cirurgia das metáses pulmonares." [Surgery of pulmonary metastasis]. Revista portuguesa de cirurgia cardio-toracica e vascular : orgao oficial da Sociedade Portuguesa de Cirurgia Cardio-Toracica e Vascular vol. 15,1 (2008): 15-7.
Cruz J, Felizardo M, Silva J, Monteiro F, Rodrigues T, Caldeira J, Costa L, Cravino J. Cirurgia das metáses pulmonares. [Surgery of pulmonary metastasis]. Rev Port Cir Cardiotorac Vasc. 2008 Jan-Mar;15(1):15-7. Portuguese. PMID: 18618046.
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First results of the Phase II SIMPLE dark matter search.

Physical review letters

Felizardo M, Morlat T, Fernandes AC, Girard TA, Marques JG, Ramos AR, Auguste M, Boyer D, Cavaillou A, Sudre C, Poupeney J, Payne RF, Miley HS, Puibasset J.
PMID: 21231283
Phys Rev Lett. 2010 Nov 19;105(21):211301. doi: 10.1103/PhysRevLett.105.211301. Epub 2010 Nov 16.

We report results of a 14.1   kg d measurement with 15 superheated droplet detectors of total active mass 0.208 kg, comprising the first stage of a 30 kg d Phase II experiment. In combination with the results of the...

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Felizardo M, Morlat T, Fernandes AC, et al. First results of the Phase II SIMPLE dark matter search. Phys Rev Lett. 2010;105(21):211301doi: 10.1103/PhysRevLett.105.211301.
Felizardo, M., Morlat, T., Fernandes, A. C., Girard, T. A., Marques, J. G., Ramos, A. R., Auguste, M., Boyer, D., Cavaillou, A., Sudre, C., Poupeney, J., Payne, R. F., Miley, H. S., Puibasset, J. (2010). First results of the Phase II SIMPLE dark matter search. Physical review letters, 105(21), 211301. https://doi.org/10.1103/PhysRevLett.105.211301
Felizardo, M, et al. "First results of the Phase II SIMPLE dark matter search." Physical review letters vol. 105,21 (2010): 211301. doi: https://doi.org/10.1103/PhysRevLett.105.211301
Felizardo M, Morlat T, Fernandes AC, Girard TA, Marques JG, Ramos AR, Auguste M, Boyer D, Cavaillou A, Sudre C, Poupeney J, Payne RF, Miley HS, Puibasset J. First results of the Phase II SIMPLE dark matter search. Phys Rev Lett. 2010 Nov 19;105(21):211301. doi: 10.1103/PhysRevLett.105.211301. Epub 2010 Nov 16. PMID: 21231283.
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A quantitative method for assessing smoke associated molecular damage in lung cancers.

Translational lung cancer research

Song K, Bi JH, Qiu ZW, Felizardo R, Girard L, Minna JD, Gazdar AF.
PMID: 30225209
Transl Lung Cancer Res. 2018 Aug;7(4):439-449. doi: 10.21037/tlcr.2018.07.01.

BACKGROUND: While tobacco exposure is the cause of the vast majority of lung cancers, an important percentage arise in lifetime never smokers. Documenting the precise extent of tobacco induced molecular changes may be of importance. Also, the contribution of...

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Song K, Bi JH, Qiu ZW, et al. A quantitative method for assessing smoke associated molecular damage in lung cancers. Transl Lung Cancer Res. 2018;7(4):439-449doi: 10.21037/tlcr.2018.07.01.
Song, K., Bi, J. H., Qiu, Z. W., Felizardo, R., Girard, L., Minna, J. D., & Gazdar, A. F. (2018). A quantitative method for assessing smoke associated molecular damage in lung cancers. Translational lung cancer research, 7(4), 439-449. https://doi.org/10.21037/tlcr.2018.07.01
Song, Kai, et al. "A quantitative method for assessing smoke associated molecular damage in lung cancers." Translational lung cancer research vol. 7,4 (2018): 439-449. doi: https://doi.org/10.21037/tlcr.2018.07.01
Song K, Bi JH, Qiu ZW, Felizardo R, Girard L, Minna JD, Gazdar AF. A quantitative method for assessing smoke associated molecular damage in lung cancers. Transl Lung Cancer Res. 2018 Aug;7(4):439-449. doi: 10.21037/tlcr.2018.07.01. PMID: 30225209; PMCID: PMC6131178.
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Microwave plasma-based direct synthesis of free-standing N-graphene.

Physical chemistry chemical physics : PCCP

Tsyganov D, Bundaleska N, Dias A, Henriques J, Felizardo E, Abrashev M, Kissovski J, do Rego AMB, Ferraria AM, Tatarova E.
PMID: 32066999
Phys Chem Chem Phys. 2020 Feb 28;22(8):4772-4787. doi: 10.1039/c9cp05509f. Epub 2020 Feb 18.

Free-standing N-graphene was synthesized using a microwave plasma-based method at atmospheric pressure conditions through a single step and in a controllable manner. Using ethanol and ammonia as precursors, N-graphene with low relative amount of bonded oxygen and low level...

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Tsyganov D, Bundaleska N, Dias A, et al. Microwave plasma-based direct synthesis of free-standing N-graphene. Phys Chem Chem Phys. 2020;22(8):4772-4787doi: 10.1039/c9cp05509f.
Tsyganov, D., Bundaleska, N., Dias, A., Henriques, J., Felizardo, E., Abrashev, M., Kissovski, J., do Rego, A. M. B., Ferraria, A. M., & Tatarova, E. (2020). Microwave plasma-based direct synthesis of free-standing N-graphene. Physical chemistry chemical physics : PCCP, 22(8), 4772-4787. https://doi.org/10.1039/c9cp05509f
Tsyganov, D, et al. "Microwave plasma-based direct synthesis of free-standing N-graphene." Physical chemistry chemical physics : PCCP vol. 22,8 (2020): 4772-4787. doi: https://doi.org/10.1039/c9cp05509f
Tsyganov D, Bundaleska N, Dias A, Henriques J, Felizardo E, Abrashev M, Kissovski J, do Rego AMB, Ferraria AM, Tatarova E. Microwave plasma-based direct synthesis of free-standing N-graphene. Phys Chem Chem Phys. 2020 Feb 28;22(8):4772-4787. doi: 10.1039/c9cp05509f. Epub 2020 Feb 18. PMID: 32066999.
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Proteomic Determination of Metabolic Protein Expression in Ten Different Tumor Cell Lines.

Cancer genomics & proteomics

Gruber-Olipitz M, Afjehi-Sadat L, Felizardo M, Slavc I, Lubec G.
PMID: 31394609
Cancer Genomics Proteomics. 2004 Jul-Aug;1(4):311-338. Epub 2004 Jul 01.

Alterations in different metabolic pathways are a prerequisite to facilitate malignant features such as invasiveness, metastasis, progression and resistance mechanisms to therapy in tumor cells. To generate metabolic protein expression patterns of tumor cells, and in order to provide...

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Gruber-Olipitz M, Afjehi-Sadat L, Felizardo M, et al. Proteomic Determination of Metabolic Protein Expression in Ten Different Tumor Cell Lines. Cancer Genomics Proteomics. 2004;1(4):311-338
Gruber-Olipitz, M., Afjehi-Sadat, L., Felizardo, M., Slavc, I., & Lubec, G. (2004). Proteomic Determination of Metabolic Protein Expression in Ten Different Tumor Cell Lines. Cancer genomics & proteomics, 1(4), 311-338.
Gruber-Olipitz, Mariella, et al. "Proteomic Determination of Metabolic Protein Expression in Ten Different Tumor Cell Lines." Cancer genomics & proteomics vol. 1,4 (2004): 311-338.
Gruber-Olipitz M, Afjehi-Sadat L, Felizardo M, Slavc I, Lubec G. Proteomic Determination of Metabolic Protein Expression in Ten Different Tumor Cell Lines. Cancer Genomics Proteomics. 2004 Jul-Aug;1(4):311-338. Epub 2004 Jul 01. PMID: 31394609.
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Obesity in kidney disease: A heavyweight opponent.

World journal of nephrology

Felizardo RJ, da Silva MB, Aguiar CF, Câmara NO.
PMID: 25332896
World J Nephrol. 2014 Aug 06;3(3):50-63. doi: 10.5527/wjn.v3.i3.50.

Obesity is an important worldwide challenge that must be faced in most developed and developing countries because of unhealthy nutritional habits. The consequences of obesity and being overweight are observed in different organs, but the kidney is one of...

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Felizardo RJ, da Silva MB, Aguiar CF, et al. Obesity in kidney disease: A heavyweight opponent. World J Nephrol. 2014;3(3):50-63doi: 10.5527/wjn.v3.i3.50.
Felizardo, R. J., da Silva, M. B., Aguiar, C. F., & Câmara, N. O. (2014). Obesity in kidney disease: A heavyweight opponent. World journal of nephrology, 3(3), 50-63. https://doi.org/10.5527/wjn.v3.i3.50
Felizardo, Raphael Jose Ferreira, et al. "Obesity in kidney disease: A heavyweight opponent." World journal of nephrology vol. 3,3 (2014): 50-63. doi: https://doi.org/10.5527/wjn.v3.i3.50
Felizardo RJ, da Silva MB, Aguiar CF, Câmara NO. Obesity in kidney disease: A heavyweight opponent. World J Nephrol. 2014 Aug 06;3(3):50-63. doi: 10.5527/wjn.v3.i3.50. PMID: 25332896; PMCID: PMC4202492.
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Final analysis and results of the Phase II SIMPLE dark matter search.

Physical review letters

Felizardo M, Girard TA, Morlat T, Fernandes AC, Ramos AR, Marques JG, Kling A, Puibasset J, Auguste M, Boyer D, Cavaillou A, Poupeney J, Sudre C, Miley HS, Payne RF, Carvalho FP, Prudêncio MI, Gouveia A, Marques R.
PMID: 23003137
Phys Rev Lett. 2012 May 18;108(20):201302. doi: 10.1103/PhysRevLett.108.201302. Epub 2012 May 18.

We report the final results of the Phase II SIMPLE measurements, comprising two run stages of 15 superheated droplet detectors each, with the second stage including an improved neutron shielding. The analyses include a refined signal analysis, and revised...

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Felizardo M, Girard TA, Morlat T, et al. Final analysis and results of the Phase II SIMPLE dark matter search. Phys Rev Lett. 2012;108(20):201302doi: 10.1103/PhysRevLett.108.201302.
Felizardo, M., Girard, T. A., Morlat, T., Fernandes, A. C., Ramos, A. R., Marques, J. G., Kling, A., Puibasset, J., Auguste, M., Boyer, D., Cavaillou, A., Poupeney, J., Sudre, C., Miley, H. S., Payne, R. F., Carvalho, F. P., Prudêncio, M. I., Gouveia, A., Marques, R. (2012). Final analysis and results of the Phase II SIMPLE dark matter search. Physical review letters, 108(20), 201302. https://doi.org/10.1103/PhysRevLett.108.201302
Felizardo, M, et al. "Final analysis and results of the Phase II SIMPLE dark matter search." Physical review letters vol. 108,20 (2012): 201302. doi: https://doi.org/10.1103/PhysRevLett.108.201302
Felizardo M, Girard TA, Morlat T, Fernandes AC, Ramos AR, Marques JG, Kling A, Puibasset J, Auguste M, Boyer D, Cavaillou A, Poupeney J, Sudre C, Miley HS, Payne RF, Carvalho FP, Prudêncio MI, Gouveia A, Marques R. Final analysis and results of the Phase II SIMPLE dark matter search. Phys Rev Lett. 2012 May 18;108(20):201302. doi: 10.1103/PhysRevLett.108.201302. Epub 2012 May 18. PMID: 23003137.
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Prospects for microwave plasma synthesized N-graphene in secondary electron emission mitigation applications.

Scientific reports

Bundaleska N, Dias A, Bundaleski N, Felizardo E, Henriques J, Tsyganov D, Abrashev M, Valcheva E, Kissovski J, Ferraria AM, do Rego AMB, Almeida A, Zavašnik J, Cvelbar U, Teodoro OMND, Strunskus T, Tatarova E.
PMID: 32747630
Sci Rep. 2020 Aug 03;10(1):13013. doi: 10.1038/s41598-020-69844-9.

The ability to change the secondary electron emission properties of nitrogen-doped graphene (N-graphene) has been demonstrated. To this end, a novel microwave plasma-enabled scalable route for continuous and controllable fabrication of free-standing N-graphene sheets was developed. High-quality N-graphene with...

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Bundaleska N, Dias A, Bundaleski N, et al. Prospects for microwave plasma synthesized N-graphene in secondary electron emission mitigation applications. Sci Rep. 2020;10(1):13013doi: 10.1038/s41598-020-69844-9.
Bundaleska, N., Dias, A., Bundaleski, N., Felizardo, E., Henriques, J., Tsyganov, D., Abrashev, M., Valcheva, E., Kissovski, J., Ferraria, A. M., do Rego, A. M. B., Almeida, A., Zavašnik, J., Cvelbar, U., Teodoro, O. M. N. D., Strunskus, T., & Tatarova, E. (2020). Prospects for microwave plasma synthesized N-graphene in secondary electron emission mitigation applications. Scientific reports, 10(1), 13013. https://doi.org/10.1038/s41598-020-69844-9
Bundaleska, N, et al. "Prospects for microwave plasma synthesized N-graphene in secondary electron emission mitigation applications." Scientific reports vol. 10,1 (2020): 13013. doi: https://doi.org/10.1038/s41598-020-69844-9
Bundaleska N, Dias A, Bundaleski N, Felizardo E, Henriques J, Tsyganov D, Abrashev M, Valcheva E, Kissovski J, Ferraria AM, do Rego AMB, Almeida A, Zavašnik J, Cvelbar U, Teodoro OMND, Strunskus T, Tatarova E. Prospects for microwave plasma synthesized N-graphene in secondary electron emission mitigation applications. Sci Rep. 2020 Aug 03;10(1):13013. doi: 10.1038/s41598-020-69844-9. PMID: 32747630; PMCID: PMC7398926.
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The microbiota and chronic kidney diseases: a double-edged sword.

Clinical & translational immunology

Felizardo RJ, Castoldi A, Andrade-Oliveira V, Câmara NO.
PMID: 27757226
Clin Transl Immunology. 2016 Jun 10;5(6):e86. doi: 10.1038/cti.2016.36. eCollection 2016 Jun.

Recent findings regarding the influence of the microbiota in many inflammatory processes have provided a new way to treat diseases. Now, one may hypothesize that the origin of a plethora of diseases is related to the health of the...

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Felizardo RJ, Castoldi A, Andrade-Oliveira V, et al. The microbiota and chronic kidney diseases: a double-edged sword. Clin Transl Immunology. 2016;5(6):e86doi: 10.1038/cti.2016.36.
Felizardo, R. J., Castoldi, A., Andrade-Oliveira, V., & Câmara, N. O. (2016). The microbiota and chronic kidney diseases: a double-edged sword. Clinical & translational immunology, 5(6), e86. https://doi.org/10.1038/cti.2016.36
Felizardo, Raphael Jose Ferreira, et al. "The microbiota and chronic kidney diseases: a double-edged sword." Clinical & translational immunology vol. 5,6 (2016): e86. doi: https://doi.org/10.1038/cti.2016.36
Felizardo RJ, Castoldi A, Andrade-Oliveira V, Câmara NO. The microbiota and chronic kidney diseases: a double-edged sword. Clin Transl Immunology. 2016 Jun 10;5(6):e86. doi: 10.1038/cti.2016.36. eCollection 2016 Jun. PMID: 27757226; PMCID: PMC5067952.
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Lack of FAM20A, Ectopic Gingival Mineralization and Chondro/Osteogenic Modifications in Enamel Renal Syndrome.

Frontiers in cell and developmental biology

Simancas Escorcia V, Diarra A, Naveau A, Dessombz A, Felizardo R, Cannaya V, Chatziantoniou C, Quentric M, Vikkula M, Cases O, Berdal A, De La Dure-Molla M, Kozyraki R.
PMID: 33425910
Front Cell Dev Biol. 2020 Dec 08;8:605084. doi: 10.3389/fcell.2020.605084. eCollection 2020.

Enamel renal syndrome (ERS) is a rare recessive disorder caused by loss-of-function mutations in

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Simancas Escorcia V, Diarra A, Naveau A, et al. Lack of FAM20A, Ectopic Gingival Mineralization and Chondro/Osteogenic Modifications in Enamel Renal Syndrome. Front Cell Dev Biol. 2020;8:605084doi: 10.3389/fcell.2020.605084.
Simancas Escorcia, V., Diarra, A., Naveau, A., Dessombz, A., Felizardo, R., Cannaya, V., Chatziantoniou, C., Quentric, M., Vikkula, M., Cases, O., Berdal, A., De La Dure-Molla, M., & Kozyraki, R. (2020). Lack of FAM20A, Ectopic Gingival Mineralization and Chondro/Osteogenic Modifications in Enamel Renal Syndrome. Frontiers in cell and developmental biology, 8605084. https://doi.org/10.3389/fcell.2020.605084
Simancas Escorcia, Victor, et al. "Lack of FAM20A, Ectopic Gingival Mineralization and Chondro/Osteogenic Modifications in Enamel Renal Syndrome." Frontiers in cell and developmental biology vol. 8 (2020): 605084. doi: https://doi.org/10.3389/fcell.2020.605084
Simancas Escorcia V, Diarra A, Naveau A, Dessombz A, Felizardo R, Cannaya V, Chatziantoniou C, Quentric M, Vikkula M, Cases O, Berdal A, De La Dure-Molla M, Kozyraki R. Lack of FAM20A, Ectopic Gingival Mineralization and Chondro/Osteogenic Modifications in Enamel Renal Syndrome. Front Cell Dev Biol. 2020 Dec 08;8:605084. doi: 10.3389/fcell.2020.605084. eCollection 2020. PMID: 33425910; PMCID: PMC7793853.
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Towards large-scale in free-standing graphene and N-graphene sheets.

Scientific reports

Tatarova E, Dias A, Henriques J, Abrashev M, Bundaleska N, Kovacevic E, Bundaleski N, Cvelbar U, Valcheva E, Arnaudov B, do Rego AMB, Ferraria AM, Berndt J, Felizardo E, Teodoro OMND, Strunskus T, Alves LL, Gonçalves B.
PMID: 28860575
Sci Rep. 2017 Aug 31;7(1):10175. doi: 10.1038/s41598-017-10810-3.

One of the greatest challenges in the commercialization of graphene and derivatives is production of high quality material in bulk quantities at low price and in a reproducible manner. The very limited control, or even lack of, over the...

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Tatarova E, Dias A, Henriques J, et al. Towards large-scale in free-standing graphene and N-graphene sheets. Sci Rep. 2017;7(1):10175doi: 10.1038/s41598-017-10810-3.
Tatarova, E., Dias, A., Henriques, J., Abrashev, M., Bundaleska, N., Kovacevic, E., Bundaleski, N., Cvelbar, U., Valcheva, E., Arnaudov, B., do Rego, A. M. B., Ferraria, A. M., Berndt, J., Felizardo, E., Teodoro, O. M. N. D., Strunskus, T., Alves, L. L., & Gonçalves, B. (2017). Towards large-scale in free-standing graphene and N-graphene sheets. Scientific reports, 7(1), 10175. https://doi.org/10.1038/s41598-017-10810-3
Tatarova, E, et al. "Towards large-scale in free-standing graphene and N-graphene sheets." Scientific reports vol. 7,1 (2017): 10175. doi: https://doi.org/10.1038/s41598-017-10810-3
Tatarova E, Dias A, Henriques J, Abrashev M, Bundaleska N, Kovacevic E, Bundaleski N, Cvelbar U, Valcheva E, Arnaudov B, do Rego AMB, Ferraria AM, Berndt J, Felizardo E, Teodoro OMND, Strunskus T, Alves LL, Gonçalves B. Towards large-scale in free-standing graphene and N-graphene sheets. Sci Rep. 2017 Aug 31;7(1):10175. doi: 10.1038/s41598-017-10810-3. PMID: 28860575; PMCID: PMC5579263.
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