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Production of parvovirus B19 vaccine in insect cells co-infected with double baculoviruses.

Biotechnology and bioengineering

Tsao EI, Mason MR, Cacciuttolo MA, Bowen SH, Folena-Wasserman G.
PMID: 18623563
Biotechnol Bioeng. 1996 Jan 20;49(2):130-8. doi: 10.1002/(SICI)1097-0290(19960120)49:2<130::AID-BIT2>3.0.CO;2-R.

Recombinant human parvovirus B19 virus-like particles (VLPs), a candidate vaccine, were produced using the insect cell (Sf-9)-baculovirus (AcNPV) expression system. The synthesis and assembly of the particles in Sf-9 cells are directed by double infections with one recombinant virus...

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