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Showing 1 to 7 of 7 entries
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Organic mercury solid phase chemoselective capture for proteomic identification of S-nitrosated proteins and peptides.

Nitric oxide : biology and chemistry

Doulias PT, Tenopoulou M, Zakopoulos I, Ischiropoulos H.
PMID: 34536587
Nitric Oxide. 2021 Dec 01;117:1-6. doi: 10.1016/j.niox.2021.09.004. Epub 2021 Sep 15.

Cysteine S-nitrosation mediates NO signaling and protein function under pathophysiological conditions. Herein, we provide a detailed protocol regarding the organic mercury chemoselective enrichment of S-nitrosated proteins and peptides. We discuss key aspects of the enrichment strategy and provide technical...

TCA cycle metabolic compromise due to an aberrant S-nitrosoproteome in HIV-associated neurocognitive disorder with methamphetamine use.

Journal of neurovirology

Doulias PT, Nakamura T, Scott H, McKercher SR, Sultan A, Deal A, Albertolle M, Ischiropoulos H, Lipton SA.
PMID: 33876414
J Neurovirol. 2021 Jun;27(3):367-378. doi: 10.1007/s13365-021-00970-4. Epub 2021 Apr 19.

In the brain, both HIV-1 and methamphetamine (meth) use result in increases in oxidative and nitrosative stress. This redox stress is thought to contribute to the pathogenesis of HIV-associated neurocognitive disorder (HAND) and further worsening cognitive activity in the...

The Convergence of Dopamine and α-Synuclein: Implications for Parkinson's Disease.

Journal of experimental neuroscience

Mor DE, Ischiropoulos H.
PMID: 29559809
J Exp Neurosci. 2018 Mar 08;12:1179069518761360. doi: 10.1177/1179069518761360. eCollection 2018.

In Parkinson's disease (PD), the loss of dopamine-producing neurons in the substantia nigra (SN) leads to severe motor impairment, and pathological inclusions known as Lewy bodies contain aggregated α-synuclein protein. The relationship of α-synuclein aggregation and dopaminergic degeneration is...

Oxidative modifications, mitochondrial dysfunction, and impaired protein degradation in Parkinson's disease: how neurons are lost in the Bermuda triangle.

Molecular neurodegeneration

Malkus KA, Tsika E, Ischiropoulos H.
PMID: 19500376
Mol Neurodegener. 2009 Jun 05;4:24. doi: 10.1186/1750-1326-4-24.

While numerous hypotheses have been proposed to explain the molecular mechanisms underlying the pathogenesis of neurodegenerative diseases, the theory of oxidative stress has received considerable support. Although many correlations have been established and encouraging evidence has been obtained, conclusive...

Correction to: TCA cycle metabolic compromise due to an aberrant S‑nitrosoproteome in HIV‑associated neurocognitive disorder with methamphetamine use.

Journal of neurovirology

Doulias PT, Nakamura T, Scott H, McKercher SR, Sultan A, Deal A, Albertolle M, Ischiropoulos H, Lipton SA.
PMID: 33942273
J Neurovirol. 2021 Jun;27(3):379. doi: 10.1007/s13365-021-00985-x.

No abstract available.

Detection of peroxynitrite in biological fluids.

Methods in molecular medicine

Malcolm S, Foust R, Hertkorn C, Ischiropoulos H.
PMID: 21340973
Methods Mol Med. 2000;36:171-7. doi: 10.1385/1-59259-216-3:171.

Peroxynitrite (ONOO(-)) is both an oxidant and a nitrating agent (1-3). However, unlike other strong oxidants, peroxynitrite reacts selectively with biological targets. This selectivity is derived in part from the different second-order rate constants (vary from 10(3)-10(6) M (-1)...

ASS1 and ASL suppress growth in clear cell renal cell carcinoma via altered nitrogen metabolism.

Cancer & metabolism

Khare S, Kim LC, Lobel G, Doulias PT, Ischiropoulos H, Nissim I, Keith B, Simon MC.
PMID: 34861885
Cancer Metab. 2021 Dec 03;9(1):40. doi: 10.1186/s40170-021-00271-8.

BACKGROUND: Kidney cancer is a common adult malignancy in the USA. Clear cell renal cell carcinoma (ccRCC), the predominant subtype of kidney cancer, is characterized by widespread metabolic changes. Urea metabolism is one such altered pathway in ccRCC. The...

Showing 1 to 7 of 7 entries