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Showing 1 to 12 of 60 entries
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Culture conditions defining glioblastoma cells behavior: what is the impact for novel discoveries?.

Oncotarget

Ledur PF, Onzi GR, Zong H, Lenz G.
PMID: 28978189
Oncotarget. 2017 Aug 11;8(40):69185-69197. doi: 10.18632/oncotarget.20193. eCollection 2017 Sep 15.

In cancer research, the use of established cell lines has gradually been replaced by primary cell cultures due to their better representation of

Thermal rearrangement of 7-methylbicyclo.

The Journal of organic chemistry

Bender JD, Leber PA, Lirio RR, Smith RS.
PMID: 10993371
J Org Chem. 2000 Aug 25;65(17):5396-402. doi: 10.1021/jo9918899.

The gas-phase thermal rearrangement of exo-7-methylbicyclo[3.2.0]hept-2-ene yields almost exclusively 5-methylnorbornene products. Inversion (i) of configuration dominates this [1,3] sigmatropic shift although some retention (r) is also observed. Because the [1,3] migration can only occur suprafacially (s) in this geometrically...

New thermal reactions of deuterium-labeled bicyclo[3.2.O]hept-2-enes: bicyclic skeletal inversion and epimerization at C7.

Journal of the American Chemical Society

Baldwin JE, Leber PA.
PMID: 11516289
J Am Chem Soc. 2001 Aug 29;123(34):8396-7. doi: 10.1021/ja011230w.

No abstract available.

Kinetics and activation parameters for the thermal 1,5-hydrogen shifts interconverting the four monodeuterio-cis,cis-1,3-cyclooctadienes.

The Journal of organic chemistry

Baldwin JE, Leber PA, Lee TW.
PMID: 11463288
J Org Chem. 2001 Jul 27;66(15):5269-71. doi: 10.1021/jo010389o.

No abstract available.

Cryptic Species in the Anopheles (Nyssorhynchus) albitarsis (Diptera: Culicidae) Complex: Incongruence Between Random Amplified Polymorphic DNA-Polymerase Chain Reaction Identification and Analysis of Mitochondrial DNA COI Gene Sequences.

Annals of the Entomological Society of America

Lehr MA, Kilpatrick CW, Wilkerson RC, Conn JE.
PMID: 17082822
Ann Entomol Soc Am. 2005 Nov;98(6):908-917. doi: 10.1603/0013-8746(2005)098[0908:CSITAN]2.0.CO;2.

Random amplified polymorphic DNA (RAPD) diagnostic bands are one tool used to differentiate cryptic mosquito species in the Anopheles albitarsis Complex. Monophyly of four species (A. albitarsis Lynch-Arribálzaga, A. albitarsis B, A. deaneorum Rosa-Freitas, and A. marajoara Galvão &...

Thermal reactions of 8-methylbicyclo[4.2.0]oct-2-enes: competitive diradical-mediated [1,3] sigmatropic, stereomutation, and fragmentation processes.

The Journal of organic chemistry

Bogle XS, Leber PA, McCullough LA, Powers DC.
PMID: 16238327
J Org Chem. 2005 Oct 28;70(22):8913-8. doi: 10.1021/jo051505g.

[reaction: see text] At 275 degrees C, 8-exo-methylbicyclo[4.2.0]oct-2-ene (1a) undergoes a [1,3] sigmatropic rearrangement to 5-methylbicyclo[2.2.2]oct-2-enes, of which the orbital symmetry-allowed si product is only marginally favored over the forbidden sr product; that is, si/sr is 2.4. Accompanying the...

[Change in pathology].

Revue medicale suisse

Bosman FT, Lehr HA.
PMID: 16396364
Rev Med Suisse. 2005 Nov 30;1(43):2771.

No abstract available.

Numerical simulations of the Macondo well blowout reveal strong control of oil flow by reservoir permeability and exsolution of gas.

Proceedings of the National Academy of Sciences of the United States of America

Oldenburg CM, Freifeld BM, Pruess K, Pan L, Finsterle S, Moridis GJ.
PMID: 21730177
Proc Natl Acad Sci U S A. 2012 Dec 11;109(50):20254-9. doi: 10.1073/pnas.1105165108. Epub 2011 Jul 05.

In response to the urgent need for estimates of the oil and gas flow rate from the Macondo well MC252-1 blowout, we assembled a small team and carried out oil and gas flow simulations using the TOUGH2 codes over...

Outcomes of dilation and evacuation with and without feticide by intra-cardiac potassium chloride injection: a service evaluation.

Contraception

Lohr PA, Parsons JH, Taylor J, Morroni C.
PMID: 29680767
Contraception. 2018 Apr 19; doi: 10.1016/j.contraception.2018.04.010. Epub 2018 Apr 19.

OBJECTIVE: To compare procedure duration, complications, and acceptability of dilation and evacuation (D&E) with and without feticide by intra-cardiac potassium chloride (KCL) injection.STUDY DESIGN: We evaluated outcomes with D&E at 18-24 weeks of gestation in the 6 months before...

Transposable element profiles reveal cell line identity and loss of heterozygosity in Drosophila cell culture.

Genetics

Han S, Basting PJ, Dias GB, Luhur A, Zelhof AC, Bergman CM.
PMID: 34849875
Genetics. 2021 Oct 02;219(2). doi: 10.1093/genetics/iyab113.

Cell culture systems allow key insights into biological mechanisms yet suffer from irreproducible outcomes in part because of cross-contamination or mislabeling of cell lines. Cell line misidentification can be mitigated by the use of genotyping protocols, which have been...

A novel transposable element based authentication protocol for Drosophila cell lines.

G3 (Bethesda, Md.)

Mariyappa D, Rusch DB, Han S, Luhur A, Overton D, Miller DFB, Bergman CM, Zelhof AC.
PMID: 34849844
G3 (Bethesda). 2021 Nov 25; doi: 10.1093/g3journal/jkab403. Epub 2021 Nov 25.

Drosophila cell lines are used by researchers to investigate various cell biological phenomena. It is crucial to exercise good cell culture practice. Poor handling can lead to both inter- and intraspecies cross-contamination. Prolonged culturing can lead to introduction of...

Generation of Drosophila attP containing cell lines using CRISPR-Cas9.

G3 (Bethesda, Md.)

Mariyappa D, Luhur A, Overton D, Zelhof AC.
PMID: 33963853
G3 (Bethesda). 2021 Aug 07;11(8). doi: 10.1093/g3journal/jkab161.

The generation of Drosophila stable cell lines has become invaluable for complementing in vivo experiments and as tools for genetic screens. Recent advances utilizing attP/PhiC31 integrase system has permitted the creation of Drosophila cells in which recombination mediated cassette...

Showing 1 to 12 of 60 entries