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Showing 1 to 8 of 8 entries
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Blinking Fluorescent Probes for Tubulin Nanoscopy in Living and Fixed Cells.

ACS chemical biology

Gerasimaitė RT, Bucevičius J, Kiszka KA, Schnorrenberg S, Kostiuk G, Koenen T, Lukinavičius G.
PMID: 34734690
ACS Chem Biol. 2021 Nov 19;16(11):2130-2136. doi: 10.1021/acschembio.1c00538. Epub 2021 Nov 04.

Here we report a small molecule tubulin probe for single-molecule localization microscopy (SMLM), stimulated emission depletion (STED) microscopy and MINFLUX nanoscopy, which can be used in living and fixed cells. We explored a series of taxane derivatives containing spontaneously...

Rhodamine-Hoechst positional isomers for highly efficient staining of heterochromatin.

Chemical science

Bucevičius J, Keller-Findeisen J, Gilat T, Hell SW, Lukinavičius G.
PMID: 30881625
Chem Sci. 2018 Dec 12;10(7):1962-1970. doi: 10.1039/c8sc05082a. eCollection 2019 Feb 21.

Hoechst conjugates to fluorescent dyes are popular DNA stains for live-cell imaging, but the relationship between their structure and performance remains elusive. This study of carboxyrhodamine-Hoechst 33258 conjugates reveals that a minimal change in the attachment point of the...

Parental genome unification is highly error-prone in mammalian embryos.

Cell

Cavazza T, Takeda Y, Politi AZ, Aushev M, Aldag P, Baker C, Choudhary M, Bucevičius J, Lukinavičius G, Elder K, Blayney M, Lucas-Hahn A, Niemann H, Herbert M, Schuh M.
PMID: 33964210
Cell. 2021 May 27;184(11):2860-2877.e22. doi: 10.1016/j.cell.2021.04.013. Epub 2021 May 07.

Most human embryos are aneuploid. Aneuploidy frequently arises during the early mitotic divisions of the embryo, but its origin remains elusive. Human zygotes that cluster their nucleoli at the pronuclear interface are thought to be more likely to develop...

Inside a Shell-Organometallic Catalysis Inside Encapsulin Nanoreactors.

Angewandte Chemie (International ed. in English)

Lohner P, Zmyslia M, Thurn J, Pape JK, Gerasimaitė R, Keller-Findeisen J, Groeer S, Deuringer B, Süss R, Walther A, Hell SW, Lukinavičius G, Hugel T, Jessen-Trefzer C.
PMID: 34418246
Angew Chem Int Ed Engl. 2021 Oct 25;60(44):23835-23841. doi: 10.1002/anie.202110327. Epub 2021 Oct 01.

Compartmentalization of chemical reactions inside cells are a fundamental requirement for life. Encapsulins are self-assembling protein-based nanocompartments from the prokaryotic repertoire that present a highly attractive platform for intracellular compartmentalization of chemical reactions by design. Using single-molecule Förster resonance...

Triarylmethane Fluorophores Resistant to Oxidative Photobluing.

Journal of the American Chemical Society

Butkevich AN, Bossi ML, Lukinavičius G, Hell SW.
PMID: 30562459
J Am Chem Soc. 2019 Jan 16;141(2):981-989. doi: 10.1021/jacs.8b11036. Epub 2019 Jan 02.

Spectral stability of small-molecule fluorescent probes is required for correct interpretation and reproducibility of multicolor fluorescence imaging data, in particular under high (de)excitation light intensities of super-resolution imaging or in single-molecule applications. We propose a synthetic approach to a...

Enhancing the biocompatibility of rhodamine fluorescent probes by a neighbouring group effect.

Chemical science

Bucevičius J, Kostiuk G, Gerasimaitė R, Gilat T, Lukinavičius G.
PMID: 33777348
Chem Sci. 2020 Jun 22;11(28):7313-7323. doi: 10.1039/d0sc02154g. eCollection 2020 Jul 28.

Fluorescence microscopy is an essential tool for understanding dynamic processes in living cells and organisms. However, many fluorescent probes for labelling cellular structures suffer from unspecific interactions and low cell permeability. Herein, we demonstrate that the neighbouring group effect...

Correction to "Triarylmethane Fluorophores Resistant to Oxidative Photobluing".

Journal of the American Chemical Society

Butkevich AN, Bossi ML, Lukinavičius G, Hell SW.
PMID: 31724848
J Am Chem Soc. 2019 Nov 27;141(47):18917. doi: 10.1021/jacs.9b11892. Epub 2019 Nov 14.

No abstract available.

Fluorescent dyes and probes for super-resolution microscopy of microtubules and tracheoles in living cells and tissues.

Chemical science

Lukinavičius G, Mitronova GY, Schnorrenberg S, Butkevich AN, Barthel H, Belov VN, Hell SW.
PMID: 29780462
Chem Sci. 2018 Feb 26;9(13):3324-3334. doi: 10.1039/c7sc05334g. eCollection 2018 Apr 07.

We introduce fluorogenic tubulin probes based on the recently reported fluorescent dyes (510R, 580CP, GeR and SiR) and chemotherapy agents - taxanes (docetaxel, cabazitaxel and larotaxel). The cytotoxicity of the final probe, its staining performance and specificity strongly depend...

Showing 1 to 8 of 8 entries