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Showing 1 to 12 of 18 entries
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Cumulative sedimentation analysis of Escherichia coli debris size.

Biotechnology and bioengineering

Wong HH, O'Neill BK, Middelberg AP.
PMID: 18636523
Biotechnol Bioeng. 1997 Aug 05;55(3):556-64. doi: 10.1002/(SICI)1097-0290(19970805)55:3<556::AID-BIT13>3.0.CO;2-E.

A new method to measure Escherichia coli cell debris size after homogenization is presented. It is based on cumulative sedimentation analysis under centrifugal force, coupled with Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) analysis of sedimented proteins. The effects that...

Insights into the interfacial structure-function of poly(ethylene glycol)-decorated peptide-stabilised nanoscale emulsions.

Soft matter

Tayeb HH, Piantavigna S, Howard CB, Nouwens A, Mahler SM, Middelberg APJ, He L, Holt SA, Sainsbury F.
PMID: 29038804
Soft Matter. 2017 Nov 08;13(43):7953-7961. doi: 10.1039/c7sm01614j.

The interfacial properties of nanoscale materials have profound influence on biodistribution and stability as well as the effectiveness of sophisticated surface-encoded properties such as active targeting to cell surface receptors. Tailorable nanocarrier emulsions (TNEs) are a novel class of...

Magnetic mesoporous silica nanoparticles end-capped with hydroxyapatite for pH-responsive drug release.

Journal of materials chemistry. B

Zhao CX, Yu L, Middelberg APJ.
PMID: 32261164
J Mater Chem B. 2013 Oct 07;1(37):4828-4833. doi: 10.1039/c3tb20641f. Epub 2013 Aug 07.

Mesoporous silica nanoparticles (MSNs) have emerged as one of the most promising vehicles for potential application in drug delivery. In this paper, we report a novel multifunctional nanocomposite composed of a magnetite nanocrystal core and a mesoporous silica shell...

Evaluation of baiting fipronil-loaded silica nanocapsules against termite colonies in fields.

Heliyon

Peters BC, Wibowo D, Yang GZ, Hui Y, Middelberg APJ, Zhao CX.
PMID: 31440604
Heliyon. 2019 Aug 10;5(8):e02277. doi: 10.1016/j.heliyon.2019.e02277. eCollection 2019 Aug.

Various pesticide nanocarriers have been developed. However, their pest-control applications remain limited in laboratories. Herein, we developed silica nanocapsules encapsulating fipronil (SNC) and their engineered form, poly(ethyleneimine)-coated SNC (SNC-PEI), based on recombinant catalytic modular protein D4S2 and used them...

Chemical treatment of Escherichia coli: 1. Extraction of intracellular protein from uninduced cells.

Biotechnology and bioengineering

Falconer RJ, O'Neill BK, Middelberg AP.
PMID: 18634040
Biotechnol Bioeng. 1997 Mar 05;53(5):453-8. doi: 10.1002/(SICI)1097-0290(19970305)53:5<453::AID-BIT2>3.0.CO;2-G.

Extraction of intracellular protein from Escherichia coli is traditionally achieved by mechanical disruption. A chemical treatment that destroys the integrity of the bacterial cell wall and could provide an alternative technique is examined in this study. Treatment with a...

Genetic associations at 53 loci highlight cell types and biological pathways relevant for kidney function.

Nature communications

Pattaro C, Teumer A, Gorski M, Chu AY, Li M, Mijatovic V, Garnaas M, Tin A, Sorice R, Li Y, Taliun D, Olden M, Foster M, Yang Q, Chen MH, Pers TH, Johnson AD, Ko YA, Fuchsberger C, Tayo B, Nalls M, Feitosa MF, Isaacs A, Dehghan A, d'Adamo P, Adeyemo A, Dieffenbach AK, Zonderman AB, Nolte IM, van der Most PJ, Wright AF, Shuldiner AR, Morrison AC, Hofman A, Smith AV, Dreisbach AW, Franke A, Uitterlinden AG, Metspalu A, Tonjes A, Lupo A, Robino A, Johansson Å, Demirkan A, Kollerits B, Freedman BI, Ponte B, Oostra BA, Paulweber B, Krämer BK, Mitchell BD, Buckley BM, Peralta CA, Hayward C, Helmer C, Rotimi CN, Shaffer CM, Müller C, Sala C, van Duijn CM, Saint-Pierre A, Ackermann D, Shriner D, Ruggiero D, Toniolo D, Lu Y, Cusi D, Czamara D, Ellinghaus D, Siscovick DS, Ruderfer D, Gieger C, Grallert H, Rochtchina E, Atkinson EJ, Holliday EG, Boerwinkle E, Salvi E, Bottinger EP, Murgia F, Rivadeneira F, Ernst F, Kronenberg F, Hu FB, Navis GJ, Curhan GC, Ehret GB, Homuth G, Coassin S, Thun GA, Pistis G, Gambaro G, Malerba G, Montgomery GW, Eiriksdottir G, Jacobs G, Li G, Wichmann HE, Campbell H, Schmidt H, Wallaschofski H, Völzke H, Brenner H, Kroemer HK, Kramer H, Lin H, Leach IM, Ford I, Guessous I, Rudan I, Prokopenko I, Borecki I, Heid IM, Kolcic I, Persico I, Jukema JW, Wilson JF, Felix JF, Divers J, Lambert JC, Stafford JM, Gaspoz JM, Smith JA, Faul JD, Wang JJ, Ding J, Hirschhorn JN, Attia J, Whitfield JB, Chalmers J, Viikari J, Coresh J, Denny JC, Karjalainen J, Fernandes JK, Endlich K, Butterbach K, Keene KL, Lohman K, Portas L, Launer LJ, Lyytikäinen LP, Yengo L, Franke L, Ferrucci L, Rose LM, Kedenko L, Rao M, Struchalin M, Kleber ME, Cavalieri M, Haun M, Cornelis MC, Ciullo M, Pirastu M, de Andrade M, McEvoy MA, Woodward M, Adam M, Cocca M, Nauck M, Imboden M, Waldenberger M, Pruijm M, Metzger M, Stumvoll M, Evans MK, Sale MM, Kähönen M, Boban M, Bochud M, Rheinberger M, Verweij N, Bouatia-Naji N, Martin NG, Hastie N, Probst-Hensch N, Soranzo N, Devuyst O, Raitakari O, Gottesman O, Franco OH, Polasek O, Gasparini P, Munroe PB, Ridker PM, Mitchell P, Muntner P, Meisinger C, Smit JH, Kovacs P, Wild PS, Froguel P, Rettig R, Mägi R, Biffar R, Schmidt R, Middelberg RP, Carroll RJ, Penninx BW, Scott RJ, Katz R, Sedaghat S, Wild SH, Kardia SL, Ulivi S, Hwang SJ, Enroth S, Kloiber S, Trompet S, Stengel B, Hancock SJ, Turner ST, Rosas SE, Stracke S, Harris TB, Zeller T, Zemunik T, Lehtimäki T, Illig T, Aspelund T, Nikopensius T, Esko T, Tanaka T, Gyllensten U, Völker U, Emilsson V, Vitart V, Aalto V, Gudnason V, Chouraki V, Chen WM, Igl W, März W, Koenig W, Lieb W, Loos RJ, Liu Y, Snieder H, Pramstaller PP, Parsa A, O'Connell JR, Susztak K, Hamet P, Tremblay J, de Boer IH, Böger CA, Goessling W, Chasman DI, Köttgen A, Kao WH, Fox CS.
PMID: 26831199
Nat Commun. 2016 Jan 21;7:10023. doi: 10.1038/ncomms10023.

Reduced glomerular filtration rate defines chronic kidney disease and is associated with cardiovascular and all-cause mortality. We conducted a meta-analysis of genome-wide association studies for estimated glomerular filtration rate (eGFR), combining data across 133,413 individuals with replication in up...

Interfacial engineering for silica nanocapsules.

Advances in colloid and interface science

Wibowo D, Hui Y, Middelberg AP, Zhao CX.
PMID: 27522646
Adv Colloid Interface Sci. 2016 Oct;236:83-100. doi: 10.1016/j.cis.2016.08.001. Epub 2016 Aug 07.

Silica nanocapsules have attracted significant interest due to their core-shell hierarchical structure. The core domain allows the encapsulation of various functional components such as drugs, fluorescent and magnetic nanoparticles for applications in drug delivery, imaging and sensing, and the...

Dielectrophoretic manipulation of surface-bound DNA.

IEE proceedings. Nanobiotechnology

Germishuizen WA, Walti C, Tosch P, Wirtz R, Pepper M, Davies AG, Middelberg AP.
PMID: 16468931
IEE Proc Nanobiotechnol. 2003 Nov;150(2):54-8. doi: 10.1049/ip-nbt:20031080.

Dielectrophoretic manipulation enables the positioning and orientation of DNA molecules for nanometer-scale applications. However, the dependence of the dielectrophoretic force and torque on the electric field magnitude and frequency has to be well characterised to realise fully the potential...

Bioengineering virus-like particles as vaccines.

Biotechnology and bioengineering

Lua LH, Connors NK, Sainsbury F, Chuan YP, Wibowo N, Middelberg AP.
PMID: 24347238
Biotechnol Bioeng. 2014 Mar;111(3):425-40. doi: 10.1002/bit.25159. Epub 2013 Dec 17.

Virus-like particle (VLP) technology seeks to harness the optimally tuned immunostimulatory properties of natural viruses while omitting the infectious trait. VLPs that assemble from a single protein have been shown to be safe and highly efficacious in humans, and...

Virus-like particle preparation is improved by control over capsomere-DNA interactions during chromatographic purification.

Biotechnology and bioengineering

Gerstweiler L, Bi J, Middelberg APJ.
PMID: 33484156
Biotechnol Bioeng. 2021 Apr;118(4):1707-1720. doi: 10.1002/bit.27687. Epub 2021 Feb 19.

Expression of viral capsomeres in bacterial systems and subsequent in vitro assembly into virus-like particles is a possible pathway for affordable future vaccines. However, purification is challenging as viral capsomeres show poor binding to chromatography media. In this study,...

Control of fed-batch fermentations.

Biotechnology advances

Lee J, Lee SY, Park S, Middelberg AP.
PMID: 14538142
Biotechnol Adv. 1999 Apr;17(1):29-48. doi: 10.1016/s0734-9750(98)00015-9.

Fed-batch fermentation is used to prevent or reduce substrate-associated growth inhibition by controlling nutrient supply. Here we review the advances in control of fed-batch fermentations. Simple exponential feeding and inferential methods are examined, as are newer methods based on...

Process-scale disruption of microorganisms.

Biotechnology advances

Middelberg AP.
PMID: 14536098
Biotechnol Adv. 1995;13(3):491-551. doi: 10.1016/0734-9750(95)02007-p.

Common hosts for the large-scale manufacture of biological products, such as Escherichia coli and Saccharomyces cerevisiae, do not excrete products to the medium. Effective techniques for cell disruption are therefore required. These include physical, chemical, enzymatic and mechanical methods....

Showing 1 to 12 of 18 entries