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Asymmetric hybridization in Nicotiana by fusion of irradiated protoplasts.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik

Bates GW, Hasenkampf CA, Contolini CL, Piastuch WC.
PMID: 24240331
Theor Appl Genet. 1987 Oct;74(6):718-26. doi: 10.1007/BF00247548.

Mesophyll protoplasts of a kanamycin-resistant, nopaline-positive Nicotiana plumbaginifolia seed line were inactivated by γ-irradiation and electrically fused with unirradiated mesophyll protoplasts of N. tabacum. Hybrids were selected on kanamycin and regenerated. Genetic material from N. plumbaginifolia was detected in...

Transient and stable expression of foreign DNA introduced into plant protoplasts by electroporation.

Methods in molecular biology (Clifton, N.J.)

Bates GW, Rabussay D, Piastuch W.
PMID: 21390617
Methods Mol Biol. 1990;6:309-22. doi: 10.1385/0-89603-161-6:309.

Electroporation utilizes high-voltage electric fields for cell permeabilization, This technique has been used for promoting the cellular uptake of exogenous molecules and macromolecules, including nucleotides, dyes, RNA, DNA, and even small proteins (1-7). Electroporation's useful attributes are its simplicity...

Unknown Renal Impairment: A Rare Case of Inhaled Tobramycin Induced Acute Kidney Injury in a Cystic Fibrosis Patient.

Antibiotics (Basel, Switzerland)

Miller T, Pastuch C, Garavaglia L, Gannon K, Parravani A.
PMID: 33921466
Antibiotics (Basel). 2021 Apr 12;10(4). doi: 10.3390/antibiotics10040424.

Acute kidney injury is a reversible medical condition commonly caused by nephrotoxic agents. The infrequency that a nebulized medication elicits a renal insult presents a rare diagnostic challenge. Within this case, we report a 57-year-old cystic fibrosis patient with...

Showing 1 to 3 of 3 entries