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Upscale of recombinant α-L-rhamnosidase production by Pichia pastoris Mut(S) strain.

Frontiers in microbiology

Markošová K, Weignerová L, Rosenberg M, Křen V, Rebroš M.
PMID: 26539173
Front Microbiol. 2015 Oct 19;6:1140. doi: 10.3389/fmicb.2015.01140. eCollection 2015.

Pichia pastoris is currently one of the most preferred microorganisms for recombinant enzyme production due to its efficient expression system. The advantages include the production of high amounts of recombinant proteins containing the appropriate posttranslational modifications and easy cultivation...

Facile synthesis of nitrophenyl 2-acetamido-2-deoxy-α-D-mannopyranosides from ManNAc-oxazoline.

Beilstein journal of organic chemistry

Křenek K, Simon P, Weignerová L, Fliedrová B, Kuzma M, Křen V.
PMID: 22509213
Beilstein J Org Chem. 2012;8:428-32. doi: 10.3762/bjoc.8.48. Epub 2012 Mar 20.

The synthetic procedures for a large-scale preparation of o- and p-nitrophenyl 2-acetamido-2-deoxy-α-D-mannopyranoside are described. The synthetic pathway employs the glycosylation of phenol with ManNAc oxazoline, followed by nitration of the aromatic moiety yielding a separable mixture of the o-...

Showing 1 to 2 of 2 entries