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Showing 1 to 12 of 433 entries
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Inorganic-carbon uptake by the marine diatom Phaeodactylum tricornutum.

Planta

Patel BN, Merrett MJ.
PMID: 24232554
Planta. 1986 Oct;169(2):222-7. doi: 10.1007/BF00392318.

Air-grown cells of the marine diatom Phaeodactylum tricornutum showed only 10% of the carbonic-anhydrase activity of air-grown Chlamydomonas reinhardtii. Measurement of carbonic-anhydrase activity using intact cells and cell extracts showed all activity was intracellular in Phaeodactylum. Photosynthetic oxygen evolution...

Trifluorothymidine exhibits potent antitumor activity via the induction of DNA double-strand breaks.

Experimental and therapeutic medicine

Suzuki N, Nakagawa F, Nukatsuka M, Fukushima M.
PMID: 22977515
Exp Ther Med. 2011 May;2(3):393-397. doi: 10.3892/etm.2011.244. Epub 2011 Mar 21.

TAS-102 is an oral anticancer drug composed of trifluorothymidine (TFT) and TPI (an inhibitor of thymidine phosphorylase that strongly inhibits the biodegradation of TFT). Similar to 5-fluorouracil (5FU) and 5-fluoro-2'-deoxyuridine (FdUrd), TFT also inhibits thymidylate synthase (TS), a rate-limiting...

Mimicking Tyrosine Phosphorylation in Human Cytochrome c by the Evolved tRNA Synthetase Technique.

Chemistry (Weinheim an der Bergstrasse, Germany)

Guerra-Castellano A, Díaz-Quintana A, Moreno-Beltrán B, López-Prados J, Nieto PM, Meister W, Staffa J, Teixeira M, Hildebrandt P, De la Rosa MA, Díaz-Moreno I.
PMID: 26329855
Chemistry. 2015 Oct 12;21(42):15004-12. doi: 10.1002/chem.201502019. Epub 2015 Aug 21.

Phosphorylation of tyrosine 48 of cytochrome c is related to a wide range of human diseases due to the pleiotropic role of the heme-protein in cell life and death. However, the structural conformation and physicochemical properties of phosphorylated cytochrome...

A gene cluster inChlorobium vibrioforme encoding the first enzymes of chlorophyll biosynthesis.

Photosynthesis research

Moberg PA, Avissar YJ.
PMID: 24310032
Photosynth Res. 1994 Jul;41(1):253-9. doi: 10.1007/BF02184166.

A cloned 5.8-kb genomic fragment of the green sulfur bacteriumChlorobium vibrioforme encodes the genes for three enzymes catalyzing early steps in the biosynthetic pathway of tetrapyrroles, common to chlorophyll and heme. ThehemA, hemC andhemD genes encode the enzymes glutamyl...

A comparative multidimensional LC-MS proteomic analysis reveals mechanisms for furan aldehyde detoxification in Thermoanaerobacter pseudethanolicus 39E.

Biotechnology for biofuels

Clarkson SM, Hamilton-Brehm SD, Giannone RJ, Engle NL, Tschaplinski TJ, Hettich RL, Elkins JG.
PMID: 25506391
Biotechnol Biofuels. 2014 Dec 03;7(1):165. doi: 10.1186/s13068-014-0165-z. eCollection 2014.

BACKGROUND: Chemical and physical pretreatment of lignocellulosic biomass improves substrate reactivity for increased microbial biofuel production, but also restricts growth via the release of furan aldehydes, such as furfural and 5-hydroxymethylfurfural (5-HMF). The physiological effects of these inhibitors on...

2'-Deoxycytidine kinase deficiency is a major determinant of 2-chloro-2'-deoxyadenosine resistance in lymphoid cell lines.

Clinical cancer research : an official journal of the American Association for Cancer Research

Orr RM, Talbot DC, Aherne WG, Fisher TC, Serafinowski P, Harrap KR.
PMID: 9815996
Clin Cancer Res. 1995 Apr;1(4):391-8.

2-Chloro-2'-deoxyadenosine, (CldAdo) resistance was developed in the W1L2 human B lymphoblastoid (resistance factor, 160) and L1210 murine leukemia (resistance factor, 605) cell lines by continuous exposure to CldAdo. Cross-resistance studies showed that while the variant lines generally retained sensitivities...

Different glycosylation of cadherins from human bladder non-malignant and cancer cell lines.

Cancer cell international

Przybylo M, Hoja-Lukowicz D, Litynska A, Laidler P.
PMID: 12234377
Cancer Cell Int. 2002 Jun 18;2:6. doi: 10.1186/1475-2867-2-6.

BACKGROUND: The aim of the present study was to determine whether stage of invasiveness of bladder cancer cell lines contributes to alterations in glycan pattern of their cadherins.RESULTS: Human non-malignant epithelial cell of ureter HCV29, v-raf transfected HCV29 line...

Improved protocols for quantitative determination of metabolites from biological samples using high performance ionic-exchange chromatography with conductimetric and pulsed amperometric detection.

Enzyme and microbial technology

Groussac E, Ortiz M, François J.
PMID: 10862877
Enzyme Microb Technol. 2000 Jun 01;26(9):715-723. doi: 10.1016/s0141-0229(00)00163-0.

Simple and reliable protocols are described for an extensive analysis of metabolites in extracts from different biological sources. The separation was performed by high performance ionic-exchange chromatography (HPIC) at alkaline pH using two types of chromatography columns and two...

Localization of Epstein-Barr Virus-Encoded Small RNA-1 by in situ Reverse Transcription: Demonstration of cDNA Generation in Formalin-Fixed Paraffin-Embedded Tissue Sections.

Journal of biomedical science

Wu TC, Ling Y, Kanayama MD, Huang AY, Kurman RJ.
PMID: 11725061
J Biomed Sci. 1995 Aug;2(3):249-255. doi: 10.1007/BF02253385.

Reverse transcription (RT) followed by polymerase chain reaction (RT-PCR) has been commonly used to detect viral and cellular transcripts in whole cell extracts. Application of this technique to tissue sections requires the in situ generation of cDNA. In this...

Dual Activity of a Viral Lysozyme with High Efficiency for Growth Inhibition of Erwinia amylovora.

Phytopathology

Salm H, Geider K.
PMID: 18943701
Phytopathology. 2004 Dec;94(12):1315-22. doi: 10.1094/PHYTO.2004.94.12.1315.

ABSTRACT The lysozyme from Erwinia amylovora phage PhiEa1h was investigated for its ability to inhibit growth of bacteria and compared with the lysozyme from Escherichia coli phage T4. The assays to measure lysozyme activity included cell lysis and growth...

Truncated Glycolytic System in Veillonella.

Journal of bacteriology

Rogosa M, Krichevsky MI, Bishop FS.
PMID: 16562014
J Bacteriol. 1965 Jul;90(1):164-71. doi: 10.1128/jb.90.1.164-171.1965.

Rogosa, M., (National Institutes of Health, Bethesda, Md.), M. I. Krichevsky, and F. S. Bishop. Truncated glycolytic system in Veillonella. J. Bacteriol. 90:164-171. 1965.-Intact Veillonella cells do not utilize carbohydrates for growth, nor are carbohydrates fermented. In cell extracts,...

Involvement of a cryptic ATPase activity of UvrB and its proteolysis product, UvrB* in DNA repair.

Nucleic acids research

Caron PR, Grossman L.
PMID: 16617484
Nucleic Acids Res. 1988 Oct 25;16(20):9651-62. doi: 10.1093/nar/16.20.9651.

The incision of damaged DNA by the Escherichia coli UvrABC endonuclease requires ATP hydrolysis. Although the deduced sequence of the UvrB protein suggests a putative ATP binding site, no nucleoside triphosphatase activity is demonstrable with the purified UvrB protein....

Showing 1 to 12 of 433 entries