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Calibration methods and avoidance of errors in measurement of intracellular pH (pHcyt) using the indicator bis(carboxyethyl)-5(6)-carboxyfluorescein (BCECF) in human platelets.

Journal of fluorescence

Valant PA, Haynes DH.
PMID: 24241630
J Fluoresc. 1992 Sep;2(3):191-206. doi: 10.1007/BF00866934.

Determinations of pHcyt in suspensions of human platelets using BCECF [bis(carboxyethyl)-5(6)-carboxyfluorescein] can be seriously biased by leakage of the fluorescent indicator. Two methods ("pH jump" and "Mn(2+)") are presented for determining the fraction of external indicator (B ext) and...

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Valant PA, Haynes DH. Calibration methods and avoidance of errors in measurement of intracellular pH (pHcyt) using the indicator bis(carboxyethyl)-5(6)-carboxyfluorescein (BCECF) in human platelets. J Fluoresc. 1992;2(3):191-206doi: 10.1007/BF00866934.
Valant, P. A., & Haynes, D. H. (1992). Calibration methods and avoidance of errors in measurement of intracellular pH (pHcyt) using the indicator bis(carboxyethyl)-5(6)-carboxyfluorescein (BCECF) in human platelets. Journal of fluorescence, 2(3), 191-206. https://doi.org/10.1007/BF00866934
Valant, P A, and Haynes, D H. "Calibration methods and avoidance of errors in measurement of intracellular pH (pHcyt) using the indicator bis(carboxyethyl)-5(6)-carboxyfluorescein (BCECF) in human platelets." Journal of fluorescence vol. 2,3 (1992): 191-206. doi: https://doi.org/10.1007/BF00866934
Valant PA, Haynes DH. Calibration methods and avoidance of errors in measurement of intracellular pH (pHcyt) using the indicator bis(carboxyethyl)-5(6)-carboxyfluorescein (BCECF) in human platelets. J Fluoresc. 1992 Sep;2(3):191-206. doi: 10.1007/BF00866934. PMID: 24241630.
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Photochemical activities, pigment distribution and photosynthetic unit size of subchloroplast particles isolated from synchronized cells of Scenedesmus obliquus.

Planta

Mell V, Senger H.
PMID: 24408471
Planta. 1978 Jan;143(3):315-22. doi: 10.1007/BF00392004.

Photosystem II (PS II) reactions of chloroplast particles show the same variations during the synchronous life cycle of Scenedesmus obliquus, strain D3 (Gaffron Biol. Zbl. 59, 302 1939), as the whole cells they derived from. Photosystem I (PS I)...

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Mell V, Senger H. Photochemical activities, pigment distribution and photosynthetic unit size of subchloroplast particles isolated from synchronized cells of Scenedesmus obliquus. Planta. 1978;143(3):315-22doi: 10.1007/BF00392004.
Mell, V., & Senger, H. (1978). Photochemical activities, pigment distribution and photosynthetic unit size of subchloroplast particles isolated from synchronized cells of Scenedesmus obliquus. Planta, 143(3), 315-22. https://doi.org/10.1007/BF00392004
Mell, V, and Senger, H. "Photochemical activities, pigment distribution and photosynthetic unit size of subchloroplast particles isolated from synchronized cells of Scenedesmus obliquus." Planta vol. 143,3 (1978): 315-22. doi: https://doi.org/10.1007/BF00392004
Mell V, Senger H. Photochemical activities, pigment distribution and photosynthetic unit size of subchloroplast particles isolated from synchronized cells of Scenedesmus obliquus. Planta. 1978 Jan;143(3):315-22. doi: 10.1007/BF00392004. PMID: 24408471.
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Noninvasive diagnostics of mitochondrial disorders in isolated lymphocytes with high resolution respirometry.

BBA clinical

Pecina P, Houšťková H, Mráček T, Pecinová A, Nůsková H, Tesařová M, Hansíková H, Janota J, Zeman J, Houštěk J.
PMID: 26675066
BBA Clin. 2014 Oct 01;2:62-71. doi: 10.1016/j.bbacli.2014.09.003. eCollection 2014 Dec.

BACKGROUND: Mitochondrial diseases belong to the most severe inherited metabolic disorders affecting pediatric population. Despite detailed knowledge of mtDNA mutations and progress in identification of affected nuclear genes, diagnostics of a substantial part of mitochondrial diseases relies on clinical...

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Pecina P, Houšťková H, Mráček T, et al. Noninvasive diagnostics of mitochondrial disorders in isolated lymphocytes with high resolution respirometry. BBA Clin. 2014;2:62-71doi: 10.1016/j.bbacli.2014.09.003.
Pecina, P., Houšťková, H., Mráček, T., Pecinová, A., Nůsková, H., Tesařová, M., Hansíková, H., Janota, J., Zeman, J., & Houštěk, J. (2014). Noninvasive diagnostics of mitochondrial disorders in isolated lymphocytes with high resolution respirometry. BBA clinical, 262-71. https://doi.org/10.1016/j.bbacli.2014.09.003
Pecina, Petr, et al. "Noninvasive diagnostics of mitochondrial disorders in isolated lymphocytes with high resolution respirometry." BBA clinical vol. 2 (2014): 62-71. doi: https://doi.org/10.1016/j.bbacli.2014.09.003
Pecina P, Houšťková H, Mráček T, Pecinová A, Nůsková H, Tesařová M, Hansíková H, Janota J, Zeman J, Houštěk J. Noninvasive diagnostics of mitochondrial disorders in isolated lymphocytes with high resolution respirometry. BBA Clin. 2014 Oct 01;2:62-71. doi: 10.1016/j.bbacli.2014.09.003. eCollection 2014 Dec. PMID: 26675066; PMCID: PMC4633944.
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[beta]-Glucan Synthesis in the Cotton Fiber (I. Identification of [beta]-1,4- and [beta]-1,3-Glucans Synthesized in Vitro).

Plant physiology

Okuda K, Li L, Kudlicka K, Kuga S, Brown RM.
PMID: 12231764
Plant Physiol. 1993 Apr;101(4):1131-1142. doi: 10.1104/pp.101.4.1131.

In vitro [beta]-glucan products were synthesized by digitonin-solubilized enzyme preparations from plasma membrane-enriched fractions of cotton (Gossypium hirsutum) fiber cells. The reaction mixture favoring [beta]-1,4-glucan synthesis included the following effectors: Mg2+, Ca2+, cellobiose, cyclic-3[prime]:5[prime]-GMP, and digitonin. The ethanol insoluble...

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Okuda K, Li L, Kudlicka K, et al. [beta]-Glucan Synthesis in the Cotton Fiber (I. Identification of [beta]-1,4- and [beta]-1,3-Glucans Synthesized in Vitro). Plant Physiol. 1993;101(4):1131-1142doi: 10.1104/pp.101.4.1131.
Okuda, K., Li, L., Kudlicka, K., Kuga, S., & Brown, R. M. (1993). [beta]-Glucan Synthesis in the Cotton Fiber (I. Identification of [beta]-1,4- and [beta]-1,3-Glucans Synthesized in Vitro). Plant physiology, 101(4), 1131-1142. https://doi.org/10.1104/pp.101.4.1131
Okuda, K., et al. "[beta]-Glucan Synthesis in the Cotton Fiber (I. Identification of [beta]-1,4- and [beta]-1,3-Glucans Synthesized in Vitro)." Plant physiology vol. 101,4 (1993): 1131-1142. doi: https://doi.org/10.1104/pp.101.4.1131
Okuda K, Li L, Kudlicka K, Kuga S, Brown RM. [beta]-Glucan Synthesis in the Cotton Fiber (I. Identification of [beta]-1,4- and [beta]-1,3-Glucans Synthesized in Vitro). Plant Physiol. 1993 Apr;101(4):1131-1142. doi: 10.1104/pp.101.4.1131. PMID: 12231764; PMCID: PMC160630.
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Rapid Enrichment of CHAPS-Solubilized UDP-Glucose: (1,3)-beta-Glucan (Callose) Synthase from Beta vulgaris L. by Product Entrapment : Entrapment Mechanisms and Polypeptide Characterization.

Plant physiology

Wu A, Harriman RW, Frost DJ, Read SM, Wasserman BP.
PMID: 16668453
Plant Physiol. 1991 Oct;97(2):684-92. doi: 10.1104/pp.97.2.684.

Rapid enrichment of CHAPS-solubilized UDP-glucose:(1,3)-beta-glucan (callose) synthase from storage tissue of red beet (Beta vulgaris L.) is obtained when the preparation is incubated with an enzyme assay mixture, then centrifuged and the enzyme released from the callose pellet with...

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Wu A, Harriman RW, Frost DJ, et al. Rapid Enrichment of CHAPS-Solubilized UDP-Glucose: (1,3)-beta-Glucan (Callose) Synthase from Beta vulgaris L. by Product Entrapment : Entrapment Mechanisms and Polypeptide Characterization. Plant Physiol. 1991;97(2):684-92doi: 10.1104/pp.97.2.684.
Wu, A., Harriman, R. W., Frost, D. J., Read, S. M., & Wasserman, B. P. (1991). Rapid Enrichment of CHAPS-Solubilized UDP-Glucose: (1,3)-beta-Glucan (Callose) Synthase from Beta vulgaris L. by Product Entrapment : Entrapment Mechanisms and Polypeptide Characterization. Plant physiology, 97(2), 684-92. https://doi.org/10.1104/pp.97.2.684
Wu, A, et al. "Rapid Enrichment of CHAPS-Solubilized UDP-Glucose: (1,3)-beta-Glucan (Callose) Synthase from Beta vulgaris L. by Product Entrapment : Entrapment Mechanisms and Polypeptide Characterization." Plant physiology vol. 97,2 (1991): 684-92. doi: https://doi.org/10.1104/pp.97.2.684
Wu A, Harriman RW, Frost DJ, Read SM, Wasserman BP. Rapid Enrichment of CHAPS-Solubilized UDP-Glucose: (1,3)-beta-Glucan (Callose) Synthase from Beta vulgaris L. by Product Entrapment : Entrapment Mechanisms and Polypeptide Characterization. Plant Physiol. 1991 Oct;97(2):684-92. doi: 10.1104/pp.97.2.684. PMID: 16668453; PMCID: PMC1081061.
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Synthesis of Fibrils in Vitro by a Solubilized Cellulose Synthase from Acetobacter xylinum.

Science (New York, N.Y.)

Lin FC, Brown RM, Cooper JB, Delmer DP.
PMID: 17791798
Science. 1985 Nov 15;230(4727):822-5. doi: 10.1126/science.230.4727.822.

A digitonin-solubilized cellulose synthase was prepared from Acetobacter xylinum. When this enzyme was incubated under conditions known to lead to active synthesis of 1,4-beta-D-glucan polymer (cellulose), electron microscopy revealed that clusters of fibrils were assembled within minutes. Individual fibrils...

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Lin FC, Brown RM, Cooper JB, et al. Synthesis of Fibrils in Vitro by a Solubilized Cellulose Synthase from Acetobacter xylinum. Science. 1985;230(4727):822-5doi: 10.1126/science.230.4727.822.
Lin, F. C., Brown, R. M., Cooper, J. B., & Delmer, D. P. (1985). Synthesis of Fibrils in Vitro by a Solubilized Cellulose Synthase from Acetobacter xylinum. Science (New York, N.Y.), 230(4727), 822-5. https://doi.org/10.1126/science.230.4727.822
Lin, F C, et al. "Synthesis of Fibrils in Vitro by a Solubilized Cellulose Synthase from Acetobacter xylinum." Science (New York, N.Y.) vol. 230,4727 (1985): 822-5. doi: https://doi.org/10.1126/science.230.4727.822
Lin FC, Brown RM, Cooper JB, Delmer DP. Synthesis of Fibrils in Vitro by a Solubilized Cellulose Synthase from Acetobacter xylinum. Science. 1985 Nov 15;230(4727):822-5. doi: 10.1126/science.230.4727.822. PMID: 17791798.
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Cellular Fractionation of Chlamydomonas reinhardii with Emphasis on the Isolation of the Chloroplast.

Plant physiology

Klein U, Chen C, Gibbs M, Platt-Aloia KA.
PMID: 16663028
Plant Physiol. 1983 Jun;72(2):481-7. doi: 10.1104/pp.72.2.481.

A method for cellular fractionation of Chlamydomonas reinhardii, SAG 11-32/b, and isolation of intact chloroplasts from synchronized cells of the alga is described. The procedure for cell fractionation comprises essentially four steps: (1) protoplast production with autolysine; (2) lysis...

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Klein U, Chen C, Gibbs M, et al. Cellular Fractionation of Chlamydomonas reinhardii with Emphasis on the Isolation of the Chloroplast. Plant Physiol. 1983;72(2):481-7doi: 10.1104/pp.72.2.481.
Klein, U., Chen, C., Gibbs, M., & Platt-Aloia, K. A. (1983). Cellular Fractionation of Chlamydomonas reinhardii with Emphasis on the Isolation of the Chloroplast. Plant physiology, 72(2), 481-7. https://doi.org/10.1104/pp.72.2.481
Klein, U, et al. "Cellular Fractionation of Chlamydomonas reinhardii with Emphasis on the Isolation of the Chloroplast." Plant physiology vol. 72,2 (1983): 481-7. doi: https://doi.org/10.1104/pp.72.2.481
Klein U, Chen C, Gibbs M, Platt-Aloia KA. Cellular Fractionation of Chlamydomonas reinhardii with Emphasis on the Isolation of the Chloroplast. Plant Physiol. 1983 Jun;72(2):481-7. doi: 10.1104/pp.72.2.481. PMID: 16663028; PMCID: PMC1066259.
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Photosynthetic Reactions by Lysed Protoplasts and Particle Preparations from the Blue-Green Alga, Phormidium luridum.

Plant physiology

Biggins J.
PMID: 16656676
Plant Physiol. 1967 Oct;42(10):1447-56. doi: 10.1104/pp.42.10.1447.

Reactions of photosynthetic electron transport and photophosphorylation were studied in preparations from the blue-green alga, Phormidium luridum. Osmotic lysis of protoplasts proved to be a superior technique for the production of cell-free preparations with high enzymatic activity. Such lysed...

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Biggins J. Photosynthetic Reactions by Lysed Protoplasts and Particle Preparations from the Blue-Green Alga, Phormidium luridum. Plant Physiol. 1967;42(10):1447-56doi: 10.1104/pp.42.10.1447.
Biggins, J. (1967). Photosynthetic Reactions by Lysed Protoplasts and Particle Preparations from the Blue-Green Alga, Phormidium luridum. Plant physiology, 42(10), 1447-56. https://doi.org/10.1104/pp.42.10.1447
Biggins, J. "Photosynthetic Reactions by Lysed Protoplasts and Particle Preparations from the Blue-Green Alga, Phormidium luridum." Plant physiology vol. 42,10 (1967): 1447-56. doi: https://doi.org/10.1104/pp.42.10.1447
Biggins J. Photosynthetic Reactions by Lysed Protoplasts and Particle Preparations from the Blue-Green Alga, Phormidium luridum. Plant Physiol. 1967 Oct;42(10):1447-56. doi: 10.1104/pp.42.10.1447. PMID: 16656676; PMCID: PMC1086745.
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Biosynthesis of Insoluble Glucans From Uridine-Diphosphate-d-Glucose With Enzyme Preparations From Phaseolus aureus and Lupinus albus.

Plant physiology

Flowers HM, Batra KK, Kemp J, Hassid WZ.
PMID: 16656958
Plant Physiol. 1968 Oct;43(10):1703-9. doi: 10.1104/pp.43.10.1703.

Particulate, and digitonin-solubilized, enzyme systems from Phaseolus aureus and Lupinus albus catalyze the biosynthesis of aqueous-insoluble glucans from UDP-d-glucose. The digitonin treatment greatly increases the enzymic activity of (per unit protein) both the 34,000g pellet and the supernatant liquid...

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Flowers HM, Batra KK, Kemp J, et al. Biosynthesis of Insoluble Glucans From Uridine-Diphosphate-d-Glucose With Enzyme Preparations From Phaseolus aureus and Lupinus albus. Plant Physiol. 1968;43(10):1703-9doi: 10.1104/pp.43.10.1703.
Flowers, H. M., Batra, K. K., Kemp, J., & Hassid, W. Z. (1968). Biosynthesis of Insoluble Glucans From Uridine-Diphosphate-d-Glucose With Enzyme Preparations From Phaseolus aureus and Lupinus albus. Plant physiology, 43(10), 1703-9. https://doi.org/10.1104/pp.43.10.1703
Flowers, H M, et al. "Biosynthesis of Insoluble Glucans From Uridine-Diphosphate-d-Glucose With Enzyme Preparations From Phaseolus aureus and Lupinus albus." Plant physiology vol. 43,10 (1968): 1703-9. doi: https://doi.org/10.1104/pp.43.10.1703
Flowers HM, Batra KK, Kemp J, Hassid WZ. Biosynthesis of Insoluble Glucans From Uridine-Diphosphate-d-Glucose With Enzyme Preparations From Phaseolus aureus and Lupinus albus. Plant Physiol. 1968 Oct;43(10):1703-9. doi: 10.1104/pp.43.10.1703. PMID: 16656958; PMCID: PMC1087062.
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Oxidation-reduction potentials of bound iron-sulfur proteins of photosystem I.

Proceedings of the National Academy of Sciences of the United States of America

Ke B, Hansen RE, Beinert H.
PMID: 16592113
Proc Natl Acad Sci U S A. 1973 Oct;70(10):2941-5. doi: 10.1073/pnas.70.10.2941.

Digitonin - fractionated photosystem - I subchloroplasts were titrated potentiometrically between -450 and -610 mV at pH 10. Examination of the titrated subchloroplasts by low-temperature (13 degrees K) electron paramagnetic resonance spectroscopy revealed resonances centered at values of 2.05,...

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Ke B, Hansen RE, Beinert H. Oxidation-reduction potentials of bound iron-sulfur proteins of photosystem I. Proc Natl Acad Sci U S A. 1973;70(10):2941-5doi: 10.1073/pnas.70.10.2941.
Ke, B., Hansen, R. E., & Beinert, H. (1973). Oxidation-reduction potentials of bound iron-sulfur proteins of photosystem I. Proceedings of the National Academy of Sciences of the United States of America, 70(10), 2941-5. https://doi.org/10.1073/pnas.70.10.2941
Ke, B, et al. "Oxidation-reduction potentials of bound iron-sulfur proteins of photosystem I." Proceedings of the National Academy of Sciences of the United States of America vol. 70,10 (1973): 2941-5. doi: https://doi.org/10.1073/pnas.70.10.2941
Ke B, Hansen RE, Beinert H. Oxidation-reduction potentials of bound iron-sulfur proteins of photosystem I. Proc Natl Acad Sci U S A. 1973 Oct;70(10):2941-5. doi: 10.1073/pnas.70.10.2941. PMID: 16592113; PMCID: PMC427143.
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The heterogeneity and nucleotide modulation of cholinergic muscarinic receptors are restored by poly (ethylene) glycol-6000 precipitation of solubilized atrial receptors.

Neurochemistry international

Aguilar JS, Ochoa EL.
PMID: 20493028
Neurochem Int. 1986;8(1):47-51. doi: 10.1016/0197-0186(86)90099-9.

The membrane bound muscarinic receptor exhibits heterogeneity of binding sites for agonists that are modulated by guanine nucleotides. These properties are generally lost upon receptor solubilization by several detergents, but may be recovered after rapid detergent removal by poly...

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Aguilar JS, Ochoa EL. The heterogeneity and nucleotide modulation of cholinergic muscarinic receptors are restored by poly (ethylene) glycol-6000 precipitation of solubilized atrial receptors. Neurochem Int. 1986;8(1):47-51doi: 10.1016/0197-0186(86)90099-9.
Aguilar, J. S., & Ochoa, E. L. (1986). The heterogeneity and nucleotide modulation of cholinergic muscarinic receptors are restored by poly (ethylene) glycol-6000 precipitation of solubilized atrial receptors. Neurochemistry international, 8(1), 47-51. https://doi.org/10.1016/0197-0186(86)90099-9
Aguilar, J S, and Ochoa, E L. "The heterogeneity and nucleotide modulation of cholinergic muscarinic receptors are restored by poly (ethylene) glycol-6000 precipitation of solubilized atrial receptors." Neurochemistry international vol. 8,1 (1986): 47-51. doi: https://doi.org/10.1016/0197-0186(86)90099-9
Aguilar JS, Ochoa EL. The heterogeneity and nucleotide modulation of cholinergic muscarinic receptors are restored by poly (ethylene) glycol-6000 precipitation of solubilized atrial receptors. Neurochem Int. 1986;8(1):47-51. doi: 10.1016/0197-0186(86)90099-9. PMID: 20493028.
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Precipitation of steroid ketones with digitonin.

The Biochemical journal

Haslewood GA.
PMID: 16748228
Biochem J. 1947;41(4):639-40.

No abstract available.

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Haslewood GA. Precipitation of steroid ketones with digitonin. Biochem J. 1947;41(4):639-40
Haslewood, G. A. (1947). Precipitation of steroid ketones with digitonin. The Biochemical journal, 41(4), 639-40.
Haslewood, G A. "Precipitation of steroid ketones with digitonin." The Biochemical journal vol. 41,4 (1947): 639-40.
Haslewood GA. Precipitation of steroid ketones with digitonin. Biochem J. 1947;41(4):639-40. PMID: 16748228; PMCID: PMC1258551.
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