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Showing 1 to 12 of 206 entries
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Usefulness of IS6110-based restriction fragment length polymorphism analysis in fingerprinting of Mycobacterium tuberculosis isolates in North India.

International journal of mycobacteriology

Mathuria JP, Anupurba S.
PMID: 28043537
Int J Mycobacteriol. 2016 Dec;5:S176-S177. doi: 10.1016/j.ijmyco.2016.10.041. Epub 2016 Nov 27.

OBJECTIVE/BACKGROUND: World Health Organization estimates that approximately one-third of the global community is infected with Mycobatcerium tuberculosis (MTB). Various molecular epidemiology methods were developed and found very promising for assessing the genetic diversity among MTB complex strain. The two...

Analysis of the mitochondrial DNA of the somatic hybrids of Solanum brevidens and S. tuberosum using non-radioactive digoxigenin-labelled DNA probes.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik

Xu YS, Jones MG, Karp A, Pehu E.
PMID: 24196153
Theor Appl Genet. 1993 Feb;85(8):1017-22. doi: 10.1007/BF00215042.

Mitochondrial (mt) DNAs of somatic hybrids obtained by electrical and chemical fusion of mesophyll protoplasts of S. brevidens and a dihaploid line of S. tuberosum PDH 40 were analysed by Southern hybridization using the digoxigenin-labelled mtDNA sequences nad5 or...

Combined In Situ Hybridization and Immunohistochemistry for Automated Detection of Cytomegalovirus and p53.

Molecular diagnosis : a journal devoted to the understanding of human disease through the clinical application of molecular biology

Rimsza LM, Vela EE, Frutiger YM, Richter LC, Grogan TM, Bellamy WT.
PMID: 10462575
Mol Diagn. 1996 Dec;1(4):291-296. doi: 10.1054/MODI00100291.

Background: Cytomegalovirus (CMV) infection has been shown to be associated with p53 overexpression in coronary artery restenosis. We investigated the occurance of this association in other forms of CMV infection using an automated in situ hybridization (ISH) technique. Methods...

Identification of Deletion Carriers in Duchenne/Becker Muscular Dystrophy Families Using a Digoxigenin-Labeled Quantitative Polymerase Chain Reaction Technique.

Molecular diagnosis : a journal devoted to the understanding of human disease through the clinical application of molecular biology

Eraslan S, Gökgöz N, Kirdar B.
PMID: 10462617
Mol Diagn. 1997 Dec;2(4):251-258. doi: 10.1054/MODI00200251.

Background: A quantitative polymerase chain reaction (PCR) technique based on the incorporation of digoxigenin (DIG), and visualization of the labeled fragments for the detection of deletion carriers in Duchenne/Becker muscular dystrophy families has been developed. Methods and Results: Sixty-five...

Antisense to cyclin D1 reverses the transformed phenotype of human gastric cancer cells.

World journal of gastroenterology

Chen B, Zhang XY, Zhang YJ, Zhou P, Gu Y, Fan DM.
PMID: 11819376
World J Gastroenterol. 1999 Feb;5(1):18-21. doi: 10.3748/wjg.v5.i1.18.

AIM:To further investigate the effect of cyclin D1 on the biologic behavior of cancer cells and its potential role in gene therapy of tumor.METHODS:A cyclin D1 subcloning plasmid termed BKSD1 was constructed by subcloning the human cyclin D1 cDNA...

A PCR-ELISA Method for Direct Detection of the Oyster Pathogen Haplosporidium nelsoni.

Marine biotechnology (New York, N.Y.)

Ko YT, Chan MM, Ford SE, Fong D.
PMID: 10373623
Mar Biotechnol (NY). 1999 Mar;1(2):147-154. doi: 10.1007/pl00011762.

: A rapid method, utilizing both polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA), was developed for detection of oyster MSX disease. The technique included using Haplosporidium nelsoni pathogen-specific PCR primers (based on ribosomal RNA genes), a Chelex...

Detection of c-K-ras point mutation in ovarian cancer.

International journal of gynecological cancer : official journal of the International Gynecological Cancer Society

Park JS, Kim HK, Han SK, Lee JM, Namkoong SE, Kim SJ.
PMID: 11578463
Int J Gynecol Cancer. 1995 Mar;5(2):107-111. doi: 10.1046/j.1525-1438.1995.05020107.x.

Point mutations of c-K-ras in ovarian cancer were detected by replacement of GGT of codon 12 by GAT, AGT, TGT and GTT, polymerase chain reaction, agarose gel electrophoresis and Southern blot hybridization with a digoxigenin detection system. The incidence...

Expression of alpha fetoprotein messenger RNA in BEL-7404 human hepatoma cells and effect of L-4 oxalysine on the expression.

World journal of gastroenterology

Wang XW, Xu B.
PMID: 11819302
World J Gastroenterol. 1998 Aug;4(4):294-297. doi: 10.3748/wjg.v4.i4.294.

AIM:To investigate alpha-fetoprotein (AFP) mRNA expression in BEL-7404 human hepatoma cells and the effect of L-4-oxalysine (OXL) on the expression.METHODS: Bel-7404 human hepatoma cells were maintained in RPMI 1640 media.Human AFP cDNA probe was labelled with digoxigenin-11-dUTP by the...

Induction of c-fos and interleukin-2 genes expression in the central nervous system following stressor stimuli.

Pathophysiology : the official journal of the International Society for Pathophysiology

Kazakova TB, Barabanova SV, Novikova NS, Nosov MA, Rogers V, Korneva EA.
PMID: 10825686
Pathophysiology. 2000 Apr;7(1):53-61. doi: 10.1016/s0928-4680(00)00029-8.

An injection of 5% mustard in vegetable oil, an injection of vegetable oil and needle prick into a rat leg gastrocnemius muscle were selected as stressor stimuli. Expression of c-fos mRNA and interleukin-2 (IL-2) mRNA was measured using digoxigenin...

Localization of Epstein-Barr Virus-Encoded Small RNA-1 by in situ Reverse Transcription: Demonstration of cDNA Generation in Formalin-Fixed Paraffin-Embedded Tissue Sections.

Journal of biomedical science

Wu TC, Ling Y, Kanayama MD, Huang AY, Kurman RJ.
PMID: 11725061
J Biomed Sci. 1995 Aug;2(3):249-255. doi: 10.1007/BF02253385.

Reverse transcription (RT) followed by polymerase chain reaction (RT-PCR) has been commonly used to detect viral and cellular transcripts in whole cell extracts. Application of this technique to tissue sections requires the in situ generation of cDNA. In this...

[Establishment and application of double color and fiber-FISH].

Yi chuan = Hereditas

Li YQ, Zhu CB, Yuan WZ, Wang YQ, Wu XS.
PMID: 15639808
Yi Chuan. 2003 Jan;25(1):1-4.

To determine whether genetic material in 5q13.3 breakpoint region changed in the course of establishing the cell line with a constitutional chromosomal inversion inv(5)(p13.1q13.3) associated with hairy cell leukemia, double color fluorescence in situ hybridization (FISH) on metaphase, interphase...

Improved specific detection of Vibrio cholerae in environmental water samples by culture on selective medium and colony hybridization assay with an oligonucleotide probe.

FEMS microbiology ecology

Robert-Pillot A, Baron S, Lesne J, Fournier JM, Quilici ML.
PMID: 19709209
FEMS Microbiol Ecol. 2002 Apr 01;40(1):39-46. doi: 10.1111/j.1574-6941.2002.tb00934.x.

We developed a rapid and efficient method based on culture on selective medium and colony hybridization assay for the detection of Vibrio cholerae in estuarine water samples. A 22-oligonucleotide sequence of the 16S-23S rDNA intergenic spacer region was labeled...

Showing 1 to 12 of 206 entries