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Xu YS, Jones MG, Karp A, et al. Analysis of the mitochondrial DNA of the somatic hybrids of Solanum brevidens and S. tuberosum using non-radioactive digoxigenin-labelled DNA probes. Theor Appl Genet. 1993;85(8):1017-22doi: 10.1007/BF00215042.
Xu, Y. S., Jones, M. G., Karp, A., & Pehu, E. (1993). Analysis of the mitochondrial DNA of the somatic hybrids of Solanum brevidens and S. tuberosum using non-radioactive digoxigenin-labelled DNA probes. TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 85(8), 1017-22. https://doi.org/10.1007/BF00215042
Xu, Y S, et al. "Analysis of the mitochondrial DNA of the somatic hybrids of Solanum brevidens and S. tuberosum using non-radioactive digoxigenin-labelled DNA probes." TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik vol. 85,8 (1993): 1017-22. doi: https://doi.org/10.1007/BF00215042
Xu YS, Jones MG, Karp A, Pehu E. Analysis of the mitochondrial DNA of the somatic hybrids of Solanum brevidens and S. tuberosum using non-radioactive digoxigenin-labelled DNA probes. Theor Appl Genet. 1993 Feb;85(8):1017-22. doi: 10.1007/BF00215042. PMID: 24196153.
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Theor Appl Genet. 1993 Feb;85(8):1017-22. doi: 10.1007/BF00215042.

Analysis of the mitochondrial DNA of the somatic hybrids of Solanum brevidens and S. tuberosum using non-radioactive digoxigenin-labelled DNA probes.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik

Y S Xu, M G Jones, A Karp, E Pehu

Affiliations

  1. Department of Plant Production, University of Helsinki, SF-00710, Helsinki, Finland.

PMID: 24196153 DOI: 10.1007/BF00215042

Abstract

Mitochondrial (mt) DNAs of somatic hybrids obtained by electrical and chemical fusion of mesophyll protoplasts of S. brevidens and a dihaploid line of S. tuberosum PDH 40 were analysed by Southern hybridization using the digoxigenin-labelled mtDNA sequences nad5 or orf25. In the Southern analysis of the hybrid mtDNA probed with nad5, most of the 19 hybrids analyzed had an RFLP pattern similar, but not identical, to one of the parents, S. tuberosum, PDH40. Nineteen percent of the hybrids had most of the S. brevidens fragments. Five of the hybrids had an identical RFLP pattern to either one of the parents while another two hybrids had novel RFLP patterns. Similar results were obtained by Southern analysis with orf25. These results clearly show that mtDNA rearrangements had occurred at a high frequency in the somatic hybrids. There were no differences in the frequencies of rearrangements observed between the hybrids regenerated from chemical and electrical fusions.

References

  1. Curr Genet. 1987;12(6):457-63 - PubMed
  2. Plant Cell Rep. 1986 Aug;5(4):262-5 - PubMed
  3. Theor Appl Genet. 1991 Mar;81(3):420-7 - PubMed
  4. Theor Appl Genet. 1988 Jul;76(1):113-7 - PubMed
  5. Theor Appl Genet. 1986 Feb;71(5):682-90 - PubMed
  6. Theor Appl Genet. 1988 Aug;76(2):260-6 - PubMed
  7. Theor Appl Genet. 1989 Nov;78(5):696-704 - PubMed
  8. EMBO J. 1985 Jul;4(7):1617-23 - PubMed
  9. Theor Appl Genet. 1988 Nov;76(5):760-6 - PubMed
  10. Theor Appl Genet. 1988 Dec;76(6):880-6 - PubMed
  11. Theor Appl Genet. 1987 May;74(1):15-20 - PubMed
  12. Theor Appl Genet. 1991 Feb;81(2):199-204 - PubMed
  13. Cell. 1981 Nov;26(3 Pt 1):315-23 - PubMed
  14. EMBO J. 1984 Sep;3(9):2107-13 - PubMed
  15. Theor Appl Genet. 1980 May;58(3-4):121-7 - PubMed
  16. Theor Appl Genet. 1984 Dec;69(2):211-6 - PubMed
  17. Theor Appl Genet. 1986 Sep;72(6):787-93 - PubMed
  18. Plant Cell Rep. 1990 Dec;9(8):411-4 - PubMed

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