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Rapid agarose gel electrophoretic mobility shift assay for quantitating protein: RNA interactions

Louis Stokes Alliances for Minority Participation AND Program Evaluation

[No authors listed]
GSID: Q5EPAZOZOJQJ
JA Ream, LK Lewis, KA Lewis - Analytical biochemistry, 2016 - Elsevier

Interactions between proteins and nucleic acids are frequently analyzed using electrophoretic mobility shift assays (EMSAs). This technique separates bound protein: nucleic acid complexes from free nucleic acids by electrophoresis, most commonly using polyacrylamide gels. The current study utilizes recent...

The omega subunit of Escherichia coli K-12 RNA polymerase is not required for stringent RNA control in vivo

k-12

Xiao H, Burgess R, Cashel M.
GSID: HrvQSmIcxHAJ
D Gentry, H Xiao, R Burgess, M Cashel - Journal of bacteriology, 1991 - Am Soc Microbiol

Igarashi et al. (K. Igarashi, N. Fujita, and A. Ishihama, Nucleic Acids Res. 17:8755-8765, 1989) reported that the omega (omega) subunit of Escherichia coli RNA polymerase was required for stringent control as judged by in vitro transcription assays in...

INSPIRE Project's Bill Taylor Memorial Scholarship Recipient Fulfilling Her STEM Dreams

Centers of Research Excellence in Science and Technology (CREST) and HBCU Research Infrastructure for Science and Engineering (RISE) AND Program Evaluation

[No authors listed]
GSID: k1_9xx1DkL0J
M Brinson - … Center 2021 Spring Intern Reports on Research …, 2019 - theinspireproject.org

Benkert, P., Künzli, M., & Schwede, T.(2009). QMEAN server for protein model quality estimation. Nucleic acids research, 37. https://doi. org/10.1093/nar/gkp322 Bienert, S., Waterhouse, A., de Beer, TAP, Tauriello, G., Studer, G., Bordoli, L., Schwede, T. The SWISS-MODEL Repository-new features...

Circulating nucleic acids in plasma and serum: applications in diagnostic techniques for noninvasive prenatal diagnosis.

International journal of women's health

Gahan PB.
PMID: 23637563
Int J Womens Health. 2013 Apr 17;5:177-86. doi: 10.2147/IJWH.S34442. Print 2013.

The analysis of fetal nucleic acids in maternal blood 13 years ago has led to the initiation of noninvasive methods for the early determination of fetal gender, rhesus D status, and a number of aneuploid disorders and hemoglobinopathies. Subsequently,...

Structural and Functional Characterization of RecG Helicase under Dilute and Molecular Crowding Conditions.

Journal of nucleic acids

Saxena S, Nagatoishi S, Miyoshi D, Sugimoto N.
PMID: 22919464
J Nucleic Acids. 2012;2012:392039. doi: 10.1155/2012/392039. Epub 2012 Aug 08.

In an ATP-dependent reaction, the Escherichia coli RecG helicase unwinds DNA junctions in vitro. We present evidence of a unique protein conformational change in the RecG helicase from an α-helix to a β-strand upon an ATP binding under dilute...

Protocol: a beginner's guide to the analysis of RNA-directed DNA methylation in plants.

Plant methods

Zhang H, Tang K, Wang B, Duan CG, Lang Z, Zhu JK.
PMID: 24955108
Plant Methods. 2014 Jun 14;10:18. doi: 10.1186/1746-4811-10-18. eCollection 2014.

BACKGROUND: DNA methylation is a conserved epigenetic mark that controls genome stability, development and environmental responses in many eukaryotes. DNA methylation can be guided by non-coding RNAs that include small interfering RNAs and scaffold RNAs. Although measurement of DNA...

A Coarse Grained Model for Atomic-Detailed DNA Simulations with Explicit Electrostatics.

Journal of chemical theory and computation

Dans PD, Zeida A, Machado MR, Pantano S.
PMID: 26615701
J Chem Theory Comput. 2010 May 11;6(5):1711-25. doi: 10.1021/ct900653p. Epub 2010 Apr 14.

Coarse-grain (CG) techniques allow considerable extension of the accessible size and time scales in simulations of biological systems. Although many CG representations are available for the most common biomacromolecules, very few have been reported for nucleic acids. Here, we...

Extraction of total nucleic acids from bacterial isolates using the bioMérieux NucliSENS easyMAG total nucleic acid extractor.

Annals of clinical microbiology and antimicrobials

Powell EA, Mortensen JE.
PMID: 27658494
Ann Clin Microbiol Antimicrob. 2016 Sep 22;15(1):54. doi: 10.1186/s12941-016-0168-7.

The BioMerieux NucliSENS easyMAG total nucleic acid extractor was evaluated for use on bacterial isolates in the clinical microbiology laboratory. Forty eight isolates were extracted, yielding quantifiable amounts of DNA for all isolates. The easyMAG is appropriate for DNA...

The PIN domain endonuclease Utp24 cleaves pre-ribosomal RNA at two coupled sites in yeast and humans.

Nucleic acids research

Wells GR, Weichmann F, Colvin D, Sloan KE, Kudla G, Tollervey D, Watkins NJ, Schneider C.
PMID: 27034467
Nucleic Acids Res. 2016 Jun 20;44(11):5399-409. doi: 10.1093/nar/gkw213. Epub 2016 Mar 31.

During ribosomal RNA (rRNA) maturation, cleavages at defined sites separate the mature rRNAs from spacer regions, but the identities of several enzymes required for 18S rRNA release remain unknown. PilT N-terminus (PIN) domain proteins are frequently endonucleases and the...

DBAASP v.2: an enhanced database of structure and antimicrobial/cytotoxic activity of natural and synthetic peptides.

Nucleic acids research

Pirtskhalava M, Gabrielian A, Cruz P, Griggs HL, Squires RB, Hurt DE, Grigolava M, Chubinidze M, Gogoladze G, Vishnepolsky B, Alekseyev V, Rosenthal A, Tartakovsky M.
PMID: 27060142
Nucleic Acids Res. 2016 Jul 27;44(13):6503. doi: 10.1093/nar/gkw243. Epub 2016 Apr 08.

No abstract available.

Circulating microRNAs in patients with hormone receptor-positive, metastatic breast cancer treated with dovitinib.

Clinical and translational medicine

Shivapurkar N, Vietsch EE, Carney E, Isaacs C, Wellstein A.
PMID: 28980224
Clin Transl Med. 2017 Oct 04;6(1):37. doi: 10.1186/s40169-017-0169-y.

BACKGROUND: Serial analysis of biomarkers in the circulation of patients undergoing treatment ("liquid biopsies") can provide new insights into drug effects. In particular the analysis of cell-free, circulating nucleic acids such as microRNAs (miRs) can reveal altered expression patterns...

Producing irreversible topoisomerase II-mediated DNA breaks by site-specific Pt(II)-methionine coordination chemistry.

Nucleic acids research

Wang YR, Chen SF, Wu CC, Liao YW, Lin TS, Liu KT, Chen YS, Li TK, Chien TC, Chan NL.
PMID: 28977544
Nucleic Acids Res. 2017 Oct 13;45(18):10920. doi: 10.1093/nar/gkx806.

No abstract available.

Showing 1 to 12 of 8748 entries