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Showing 1 to 12 of 30 entries
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Genotoxic Damage to Glioblastoma Cells Treated with 6 MV X-Radiation in The Presence or Absence of Methoxy Estradiol, IUDR or Topotecan.

Cell journal

Eyvazzadeh N, Neshasteh-Riz A, Mahdavi SR, Mohsenifar A.
PMID: 26199910
Cell J. 2015;17(2):312-21. doi: 10.22074/cellj.2016.3738. Epub 2015 Jul 11.

OBJECTIVE: To explore the cumulative genotoxic damage to glioblastoma (GBM) cells, grown as multicellular spheroids, following exposure to 6 MV X-rays (2 Gy, 22 Gy) with or without, 2- methoxy estradiol (2ME2), iododeoxyuridine (IUDR) or topotecan (TPT), using the...

DNA and RNA Extraction and Quantitative Real-Time PCR-Based Assays for Biogas Biocenoses in an Interlaboratory Comparison.

Bioengineering (Basel, Switzerland)

Lebuhn M, Derenkó J, Rademacher A, Helbig S, Munk B, Pechtl A, Stolze Y, Prowe S, Schwarz WH, Schlüter A, Liebl W, Klocke M.
PMID: 28952569
Bioengineering (Basel). 2016 Jan 13;3(1). doi: 10.3390/bioengineering3010007.

Five institutional partners participated in an interlaboratory comparison of nucleic acid extraction, RNA preservation and quantitative Real-Time PCR (qPCR) based assays for biogas biocenoses derived from different grass silage digesting laboratory and pilot scale fermenters. A kit format DNA...

Anti-breast cancer and anti-angiogenic potential of a lichen-derived small-molecule: barbatolic acid.

Cytotechnology

Varol M.
PMID: 30155608
Cytotechnology. 2018 Dec;70(6):1565-1573. doi: 10.1007/s10616-018-0249-x. Epub 2018 Aug 28.

Natural products have been used for centuries as the most potent remedies to cure many diseases including cancer diseases. Angiogenesis is defined as the formation of new capillaries from existing vessels and plays a key role in the tumorigenesis...

Combinations of growth factors for human mesenchymal stem cell proliferation and osteogenic differentiation.

Bone & joint research

Hefka Blahnova V, Dankova J, Rampichova M, Filova E.
PMID: 32864112
Bone Joint Res. 2020 Aug 19;9(7):412-420. doi: 10.1302/2046-3758.97.BJR-2019-0183.R2. eCollection 2020 Jul.

AIMS: Here we introduce a wide and complex study comparing effects of growth factors used alone and in combinations on human mesenchymal stem cell (hMSC) proliferation and osteogenic differentiation. Certain ways of cell behaviour can be triggered by specific...

Dataset of the absorption, emission and excitation spectra and fluorescence intensity graphs of fluorescent cyanine dyes for the quantification of low amounts of dsDNA.

Data in brief

Bruijns B, Tiggelaar R, Gardeniers H.
PMID: 27957521
Data Brief. 2016 Nov 28;10:132-143. doi: 10.1016/j.dib.2016.11.090. eCollection 2017 Feb.

This article describes data related to a research article entitled "Fluorescent cyanine dyes for the quantification of low amounts of dsDNA" (B. Bruijns, R. Tiggelaar, J. Gardeniers, 2016) [1]. Six cyanine dsDNA dyes - EvaGreen, SYBR Green, PicoGreen, AccuClear,...

Real-time PCR.

Forensic science, medicine, and pathology

Bowyer VL.
PMID: 25868892
Forensic Sci Med Pathol. 2007 Mar;3(1):61-3. doi: 10.1385/FSMP:3:1:61.

Research into the field of DNA has increased dramatically in the years since DNA profiling was first identified. Since then, techniques have been developed to identify individuals from DNA samples equivalent to a single cell. Development of these techniques...

Cryptic Streptococcus mutans 5.6-kb plasmids encode a toxin-antitoxin system for plasmid stabilization.

Journal of oral microbiology

Rheinberg A, Swierzy IJ, Nguyen TD, Horz HP, Conrads G.
PMID: 23330057
J Oral Microbiol. 2013;5. doi: 10.3402/jom.v5i0.19729. Epub 2013 Jan 15.

BACKGROUND: In all Streptococcus mutans strains, 5-13% carry a 5.6-kb plasmid. Despite its frequency, little is known about its mediated functions with most of the information coming from a single study focussing on plasmid pUA140.OBJECTIVE: Here, we describe the...

Quantitative analysis of cell-free DNA in the plasma of gastric cancer patients.

Oncology letters

Park JL, Kim HJ, Choi BY, Lee HC, Jang HR, Song KS, Noh SM, Kim SY, Han DS, Kim YS.
PMID: 22741019
Oncol Lett. 2012 Apr 01;3(4):921-926. doi: 10.3892/ol.2012.592. Epub 2012 Feb 03.

In the present study, an accurate and reproducible method for quantifying cell-free DNA (cfDNA) in human blood was established and tested for its ability to predict gastric cancer in patients. Using 'Alu81-qPCR' to amplify 81-bp Alu DNA sequences, we...

Evidence for simvastatin anti-inflammatory actions based on quantitative analyses of NETosis and other inflammation/oxidation markers.

Results in immunology

Al-Ghoul WM, Kim MS, Fazal N, Azim AC, Ali A.
PMID: 24809006
Results Immunol. 2014 Mar 25;4:14-22. doi: 10.1016/j.rinim.2014.03.001. eCollection 2014.

Simvastatin (SMV) has been shown to exhibit promising anti-inflammatory properties alongside its classic cholesterol lowering action. We tested these emerging effects in a major thermal injury mouse model (3rd degree scald, ~20% TBSA) with previously documented, inflammation-mediated intestinal defects....

Multiple-Monitor HPLC Assays for Rapid Process Development, In-Process Monitoring, and Validation of AAV Production and Purification.

Pharmaceutics

Gagnon P, Goricar B, Mencin N, Zvanut T, Peljhan S, Leskovec M, Strancar A.
PMID: 33477351
Pharmaceutics. 2021 Jan 17;13(1). doi: 10.3390/pharmaceutics13010113.

HPLC is established as a fast convenient analytical technology for characterizing the content of empty and full capsids in purified samples containing adeno-associated virus (AAV). UV-based monitoring unfortunately over-estimates the proportion of full capsids and offers little value for...

Evaluation of silk sericin as a biomaterial: in vitro growth of human corneal limbal epithelial cells on Bombyx mori sericin membranes.

Progress in biomaterials

Chirila TV, Suzuki S, Bray LJ, Barnett NL, Harkin DG.
PMID: 29470674
Prog Biomater. 2013 Nov 28;2(1):14. doi: 10.1186/2194-0517-2-14.

Sericin and fibroin are the two major proteins in the silk fibre produced by the domesticated silkworm, Bombyx mori. Fibroin has been extensively investigated as a biomaterial. We have previously shown that fibroin can function successfully as a substratum...

Pleiotropic Roles of NOTCH1 Signaling in the Loss of Maturational Arrest of Human Osteoarthritic Chondrocytes.

International journal of molecular sciences

Minguzzi M, Panichi V, D'Adamo S, Cetrullo S, Cattini L, Flamigni F, Mariani E, Borzì RM.
PMID: 34769441
Int J Mol Sci. 2021 Nov 05;22(21). doi: 10.3390/ijms222112012.

Notch signaling has been identified as a critical regulator of cartilage development and homeostasis. Its pivotal role was established by both several joint specific Notch signaling loss of function mouse models and transient or sustained overexpression. NOTCH1 is the...

Showing 1 to 12 of 30 entries