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Showing 1 to 12 of 88 entries
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Divergent evolutionary behavior of H3 histone gene and rDNA clusters in venerid clams.

Molecular cytogenetics

García-Souto D, Pérez-García C, Morán P, Pasantes JJ.
PMID: 26106449
Mol Cytogenet. 2015 Jun 24;8:40. doi: 10.1186/s13039-015-0150-7. eCollection 2015.

BACKGROUND: Histone H3 gene clusters have been described as highly conserved chromosomal markers in invertebrates. Surprisingly, in bivalves remarkable interspecific differences were found among the eight mussels and between the two clams in which histone H3 gene clusters have...

Proposal of an appropriate decalcification method of bone marrow biopsy specimens in the era of expanding genetic molecular study.

Journal of pathology and translational medicine

Choi SE, Hong SW, Yoon SO.
PMID: 26018515
J Pathol Transl Med. 2015 May;49(3):236-42. doi: 10.4132/jptm.2015.03.16. Epub 2015 May 15.

BACKGROUND: The conventional method for decalcification of bone specimens uses hydrochloric acid (HCl) and is notorious for damaging cellular RNA, DNA, and proteins, thus complicating molecular and immunohistochemical analyses. A method that can effectively decalcify while preserving genetic material...

Specific expression of myosin heavy chain gene in muscle lineage cells of the ascidian embryo.

Roux's archives of developmental biology : the official organ of the EDBO

Makabe KW, Fujiwara S, Saiga H, Satoh N.
PMID: 28306115
Rouxs Arch Dev Biol. 1990 May;199(5):307-313. doi: 10.1007/BF01709509.

In ascidians the lineage of embryonic precursor cells is well documented. In this study, we investigated temporal and spatial expression of myosin heavy chain (MHC) gene during embryogenesis of the ascidianHalocynthia roretzi. When the occurrence of MHC transcripts was...

Antisense to cyclin D1 reverses the transformed phenotype of human gastric cancer cells.

World journal of gastroenterology

Chen B, Zhang XY, Zhang YJ, Zhou P, Gu Y, Fan DM.
PMID: 11819376
World J Gastroenterol. 1999 Feb;5(1):18-21. doi: 10.3748/wjg.v5.i1.18.

AIM:To further investigate the effect of cyclin D1 on the biologic behavior of cancer cells and its potential role in gene therapy of tumor.METHODS:A cyclin D1 subcloning plasmid termed BKSD1 was constructed by subcloning the human cyclin D1 cDNA...

Expression of basic fibroblast growth factor receptor messenger RNA in the periinfarcted brain tissue.

Restorative neurology and neuroscience

Sakaguchi T, Yamada K, Wanaka A, Kohmura E, Yuguchi T, Taneda M, Kataoka K, Tohyama M, Hayakawa T.
PMID: 21551769
Restor Neurol Neurosci. 1994 Jan 01;7(1):29-36. doi: 10.3233/RNN-1994-7105.

The present study examined whether expression of basic fibroblast growth factor receptor (bFGFR) messenger ribonucleic acid (mRNA) was upregulated by focal ischemia. We have studied the in situ hybridization autoradiography for bFGFR mRNA in the rat model of middle...

Micropatterning of Aptamer Beacons to Create Cytokine-Sensing Surfaces.

Cellular and molecular bioengineering

Tuleuova N, Revzin A.
PMID: 21170394
Cell Mol Bioeng. 2010 Dec;3(4):337-344. doi: 10.1007/s12195-010-0148-5. Epub 2010 Nov 20.

Aptamer beacons are DNA or RNA probes that bind proteins or small molecules of interest and emit signal directly upon interaction with the target analyte. This paper describes micropatterning of aptamer beacons for detection of IFN-γ-an important inflammatory cytokine....

Erythropoetin receptor expression in the human diabetic retina.

BMC research notes

Shah SS, Tsang SH, Mahajan VB.
PMID: 19930719
BMC Res Notes. 2009 Nov 25;2:234. doi: 10.1186/1756-0500-2-234.

BACKGROUND: Recent evidence suggests erythropoietin (EPO) and the erythropoietin receptor (EPOR) may play a direct role in the pathogenesis of diabetic retinopathy. Better characterization of the EPO-EPOR signaling system in the ischemic retina may offer a new therapeutic modality...

In situ hybridization of mouse embryo and tissue sections with radiolabeled RNA probes.

CSH protocols

Nagy A, Gertsenstein M, Vintersten K, Behringer R.
PMID: 21356974
CSH Protoc. 2007 Nov 01;2007:pdb.prot4821. doi: 10.1101/pdb.prot4821.

INTRODUCTIONThis protocol describes in situ hybridization of embryo and tissue sections with (35)S-labeled, single-stranded, antisense RNA probes (riboprobes). Protocols have also been developed for in situ hybridization to tissue sections using nonradiolabeled RNA probes that can be detected with...

Synthesis of Single-stranded RNA Probes by In Vitro Transcription.

CSH protocols

Sambrook J, Russell DW.
PMID: 22485332
CSH Protoc. 2006 Jun 01;2006(1). doi: 10.1101/pdb.prot3847.

No abstract available.

An environmental DNA sampling method for aye-ayes from their feeding traces.

Ecology and evolution

Aylward ML, Sullivan AP, Perry GH, Johnson SE, Louis EE.
PMID: 30377496
Ecol Evol. 2018 Jul 31;8(18):9229-9240. doi: 10.1002/ece3.4341. eCollection 2018 Sep.

Noninvasive sampling is an important development in population genetic monitoring of wild animals. Particularly, the collection of environmental DNA (eDNA) which can be collected without needing to encounter the target animal facilitates the genetic analysis of endangered species. One...

Whole-mount in situ hybridization of RNA probes to plant tissues.

CSH protocols

Traas J.
PMID: 21356775
CSH Protoc. 2008 Feb 01;2008:pdb.prot4944. doi: 10.1101/pdb.prot4944.

INTRODUCTIONThe labeling of entire plantlets (whole-mount labeling) is very easy, but it can cause substantial artifacts. Because whole plants are labeled, certain tissues are not readily accessible to the probes, whereas others can often stain nonspecifically. In the hands...

Low-cost cross-taxon enrichment of mitochondrial DNA using in-house synthesised RNA probes.

PloS one

Richards SM, Hovhannisyan N, Gilliham M, Ingram J, Skadhauge B, Heiniger H, Llamas B, Mitchell KJ, Meachen J, Fincher GB, Austin JJ, Cooper A.
PMID: 30716066
PLoS One. 2019 Feb 04;14(2):e0209499. doi: 10.1371/journal.pone.0209499. eCollection 2019.

Hybridization capture with in-solution oligonucleotide probes has quickly become the preferred method for enriching specific DNA loci from degraded or ancient samples prior to high-throughput sequencing (HTS). Several companies synthesize sets of probes for in-solution hybridization capture, but these...

Showing 1 to 12 of 88 entries