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Showing 1 to 12 of 411 entries
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Long-Term Culture of Lobster Central Ganglia: Expression of Foreign Genes in Identified Neurons.

The Biological bulletin

Ganter GK, Heinrich R, Bunge RP, Kravitz EA.
PMID: 28296498
Biol Bull. 1999 Aug;197(1):40-48. doi: 10.2307/1542995.

The ventral nerve cords of lobsters (Homarus americanus) can be cultured in vitro for at least 7 weeks. Over this period, neurons maintain their normal electrophysiological features and continue, among other measures of neuronal health, to synthesize RNA and...

Microglia change from a reactive to an age-like phenotype with the time in culture.

Frontiers in cellular neuroscience

Caldeira C, Oliveira AF, Cunha C, Vaz AR, Falcão AS, Fernandes A, Brites D.
PMID: 24917789
Front Cell Neurosci. 2014 Jun 02;8:152. doi: 10.3389/fncel.2014.00152. eCollection 2014.

Age-related neurodegenerative diseases have been associated with chronic neuroinflammation and microglia activation. However, cumulative evidence supports that inflammation only occurs at an early stage once microglia change the endogenous characteristics with aging and switch to irresponsive/senescent and dystrophic phenotypes...

hnRNP C1/C2 and Pur-beta proteins mediate induction of senescence by oligonucleotides homologous to the telomere overhang.

OncoTargets and therapy

Mulnix RE, Pitman RT, Retzer A, Bertram C, Arasi K, Crees Z, Girard J, Uppada SB, Stone AL, Puri N.
PMID: 24379680
Onco Targets Ther. 2013 Dec 18;7:23-32. doi: 10.2147/OTT.S54575. eCollection 2013.

BACKGROUND: Experimental disruption of the telomere overhang induces a potent DNA damage response and is the target of newly emerging cancer therapeutics. Introduction of T-oligo, an eleven-base oligonucleotide homologous to the 3'-telomeric overhang, mimics telomere disruption and induces DNA...

β-galactosidase Production by Aspergillus niger ATCC 9142 Using Inexpensive Substrates in Solid-State Fermentation: Optimization by Orthogonal Arrays Design.

Iranian biomedical journal

Kazemi S, Khayati G, Faezi-Ghasemi M.
PMID: 27721510
Iran Biomed J. 2016;20(5):287-294. doi: 10.22045/ibj.2016.06. Epub 2016 May 25.

BACKGROUND: Enzymatic hydrolysis of lactose is one of the most important biotechnological processes in the food industry, which is accomplished by enzyme β-galactosidase (β-gal, β-D-galactoside galactohydrolase, EC 3.2.1.23), trivial called lactase. Orthogonal arrays design is an appropriate option for...

Xyloglucan mobilisation in cotyledons of developing plantlets of Hymenaea courbaril L. (Leguminosae-Caesalpinoideae).

Plant science : an international journal of experimental plant biology

Tiné MA, Cortelazzo AL, Buckeridge MS.
PMID: 10729610
Plant Sci. 2000 May 29;154(2):117-126. doi: 10.1016/s0168-9452(99)00245-9.

Many seeds contain storage compounds that are used by the embryo/plantlet as a source of nutrients after germination. In seeds of Hymenaea courbaril, a leguminous tree, the main reserve consists of a structurally unusual xyloglucan stored in thickened walls...

Vascular Applications of Human Gene Therapy.

Journal of thrombosis and thrombolysis

Pickering JG, Takeshita S, Feldman L, Losordo DW, Isner JM.
PMID: 10608008
J Thromb Thrombolysis. 1995;1(3):299-302. doi: 10.1007/BF01060740.

Complexing recombinant DNA with cationic liposomes is a convenient means of introducing foreign genes into cells (lipofection) and could potentially form the basis for genetically modifying diseased blood vessels in patients. The mechanism of lipofection is incompletely understood, but...

beta-Galactosidase immobilization for milk lactose hydrolysis: a simple experimental and modelling study of batch and continuous reactors.

Biochemical education

Obón JM, Castellar MR, Iborra JL, Manjón A.
PMID: 10878316
Biochem Educ. 2000 May 01;28(3):164-168. doi: 10.1016/s0307-4412(99)00097-7.

The experiment described in this paper introduces students to the practical use of an enzyme (beta-galactosidase, or lactase) acting on a natural substrate. The enzyme is immobilized onto a cheap support, and the immobilized derivative is used in a...

The Application of a Novel Lytic System to the Recovery of Recombinant Proteins in E.coli.

Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica

Yang YG, Tong Q, Hu TS, Qian YC, Yang SL, Gong Y.
PMID: 12075442
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 2000;32(3):211-216.

In order to efficiently recover recombinant proteins, a temperature-sensitive lytic system was constructed on the basis of the feature that T4 lysozyme disrupts the bacteria through cutting specific bond in the peptidoglycan layer of cell wall. This system was...

The Construction and Application of the Multi-copy Integration Vector in K. lactis.

Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica

Tang NY, Huo KK, Li YY.
PMID: 12232616
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 1996;28(5):540-546.

The vector pIRK was constructed by using a 2.2 kb EcoRI fragment of rDNA from Kluyveromyces lactis for targeted homologous recombination, with the URA3 gene from Saccharomyces cerevisiae acting as a selection marker. By the examination of the copy...

Rhizobium meliloti Genes Encoding Catabolism of Trigonelline Are Induced under Symbiotic Conditions.

The Plant cell

Boivin C, Camut S, Malpica CA, Truchet G, Rosenberg C.
PMID: 12354952
Plant Cell. 1990 Dec;2(12):1157-1170. doi: 10.1105/tpc.2.12.1157.

Rhizobium meliloti trc genes controlling the catabolism of trigonelline, a plant secondary metabolite often abundant in legumes, are closely linked to nif-nod genes on the symbiotic megaplasmid pSym [Boivin, C., Malpica, C., Rosenberg, C., Denarie, J., Goldman, A., Fleury,...

[Recombinant adenovirus-mediated gene transfer suppresses experimental arthritis].

Reumatismo

Quattrocchi E, Feldmann M.
PMID: 12461578
Reumatismo. 2001;53(1):46-54. doi: 10.4081/reumatismo.2001.46.

Collagen Induced Arthritis (CIA) is a widely studied animal model to develop and test novel therapeutic approaches for treating Rheumatoid Arthritis (RA) in humans. Soluble Cytotoxic T-Lymphocyte Antigen 4 (CTLA4-Ig), which binds B7 molecule on antigen presenting cells and...

Amplification of competitive telomere sequence in living animal cells induces chromatin instability.

Cytotechnology

Mizuarai S, Nishijima K, Iijima S.
PMID: 19003141
Cytotechnology. 1999 Sep;31(1):195-203. doi: 10.1023/A:1008088609398.

We have reported the establishment of new episomal-type expression vector the copy number of which can be readily regulated by a temperature shift. In this study, we attempt to apply this vector for the functional analysis of the noncoding...

Showing 1 to 12 of 411 entries