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Showing 1 to 12 of 186 entries
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Technique: Transcript tracking by CRISPR.

Nature reviews. Genetics

Burgess DJ.
PMID: 27040488
Nat Rev Genet. 2016 May;17(5):254-5. doi: 10.1038/nrg.2016.41. Epub 2016 Mar 30.

No abstract available.

CRISPR/Cas9 genome editing in human pluripotent stem cells: Harnessing human genetics in a dish.

Developmental dynamics : an official publication of the American Association of Anatomists

González F.
PMID: 27145095
Dev Dyn. 2016 Jul;245(7):788-806. doi: 10.1002/dvdy.24414. Epub 2016 Jun 09.

Because of their extraordinary differentiation potential, human pluripotent stem cells (hPSCs) can differentiate into virtually any cell type of the human body, providing a powerful platform not only for generating relevant cell types useful for cell replacement therapies, but...

CRISPR-Cas Systems Reduced to a Minimum.

Molecular cell

Almendros C, Kieper SN, Brouns SJJ.
PMID: 30794791
Mol Cell. 2019 Feb 21;73(4):641-642. doi: 10.1016/j.molcel.2019.02.005.

In two recent studies in Molecular Cell, Wright et al. (2019) report complete spacer integration by a Cas1 mini-integrase and Edraki et al. (2019) describe accurate genome editing by a small Cas9 ortholog with less stringent PAM requirements.

A RecET-assisted CRISPR-Cas9 genome editing in Corynebacterium glutamicum.

Microbial cell factories

Wang B, Hu Q, Zhang Y, Shi R, Chai X, Liu Z, Shang X, Zhang Y, Wen T.
PMID: 29685154
Microb Cell Fact. 2018 Apr 23;17(1):63. doi: 10.1186/s12934-018-0910-2.

BACKGROUND: Extensive modification of genome is an efficient manner to regulate the metabolic network for producing target metabolites or non-native products using Corynebacterium glutamicum as a cell factory. Genome editing approaches by means of homologous recombination and counter-selection markers...

CRISPR EnAbled Trackable genome Engineering for isopropanol production in Escherichia coli.

Metabolic engineering

Liang L, Liu R, Garst AD, Lee T, Nogué VSI, Beckham GT, Gill RT.
PMID: 28216108
Metab Eng. 2017 May;41:1-10. doi: 10.1016/j.ymben.2017.02.009. Epub 2017 Feb 16.

Isopropanol is an important target molecule for sustainable production of fuels and chemicals. Increases in DNA synthesis and synthetic biology capabilities have resulted in the development of a range of new strategies for the more rapid design, construction, and...

Genome Editing in Human Neural Stem and Progenitor Cells.

Results and problems in cell differentiation

Bressan RB, Pollard SM.
PMID: 30209659
Results Probl Cell Differ. 2018;66:163-182. doi: 10.1007/978-3-319-93485-3_7.

Experimental tools for precise manipulation of mammalian genomes enable reverse genetic approaches to explore biology and disease. Powerful genome editing technologies built upon designer nucleases, such as CRISPR/Cas9, have recently emerged. Parallel progress has been made in methodologies for...

High-throughput genetic screens using CRISPR-Cas9 system.

Archives of pharmacal research

Kweon J, Kim Y.
PMID: 29637495
Arch Pharm Res. 2018 Sep;41(9):875-884. doi: 10.1007/s12272-018-1029-z. Epub 2018 Apr 10.

The CRISPR-Cas9 system is a powerful tool for genome engineering, and its programmability and simplicity have enabled various types of gene manipulation such as gene disruption and transcriptional and epigenetic perturbation. Particularly, CRISPR-based pooled libraries facilitate high-throughput screening for...

Systematic analysis of Type I-E Escherichia coli CRISPR-Cas PAM sequences ability to promote interference and primed adaptation.

Molecular microbiology

Musharova O, Sitnik V, Vlot M, Savitskaya E, Datsenko KA, Krivoy A, Fedorov I, Semenova E, Brouns SJJ, Severinov K.
PMID: 30875129
Mol Microbiol. 2019 Jun;111(6):1558-1570. doi: 10.1111/mmi.14237. Epub 2019 Apr 06.

CRISPR interference occurs when a protospacer recognized by the CRISPR RNA is destroyed by Cas effectors. In Type I CRISPR-Cas systems, protospacer recognition can lead to «primed adaptation» - acquisition of new spacers from in cis located sequences. Type...

Progress and Challenges for Live-cell Imaging of Genomic Loci Using CRISPR-based Platforms.

Genomics, proteomics & bioinformatics

Wu X, Mao S, Ying Y, Krueger CJ, Chen AK.
PMID: 30710789
Genomics Proteomics Bioinformatics. 2019 Apr;17(2):119-128. doi: 10.1016/j.gpb.2018.10.001. Epub 2019 Jan 30.

Chromatin conformation, localization, and dynamics are crucial regulators of cellular behaviors. Although fluorescence in situ hybridization-based techniques have been widely utilized for investigating chromatin architectures in healthy and diseased states, the requirement for cell fixation precludes the comprehensive dynamic...

Off-target genome editing: A new discipline of gene science and a new class of medicine.

Cell biology and toxicology

Wang DC, Wang X.
PMID: 31030279
Cell Biol Toxicol. 2019 Jun;35(3):179-183. doi: 10.1007/s10565-019-09475-7. Epub 2019 Apr 27.

With an increasing growth of genome editing, off-target effects such as non-specific genetic modifications resulting from the designed process of genome editing become a new discipline of gene science and new class medicine. The degree of short-term and long-term...

Super-precise new CRISPR tool could tackle a plethora of genetic diseases.

Nature

Ledford H.
PMID: 31641267
Nature. 2019 Oct;574(7779):464-465. doi: 10.1038/d41586-019-03164-5.

No abstract available.

Finding Her Niche: An Interview with Emmanuelle Charpentier.

The CRISPR journal

Davies K, Charpentier E.
PMID: 31021229
CRISPR J. 2019 Feb;2:17-22. doi: 10.1089/crispr.2019.29042.kda.

No abstract available.

Showing 1 to 12 of 186 entries