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Efficient ligation and cloning of DNA fragments with 2-bp overhangs.

Analytical biochemistry

Ranjan RK, Rajagopal K.
PMID: 20298672
Anal Biochem. 2010 Jul 01;402(1):91-2. doi: 10.1016/j.ab.2010.03.020. Epub 2010 Mar 15.

Various methods of ligation are currently available and routinely used by molecular biologists, such as blunt end ligation, cohesive end (two and four overhangs), and ligation of Taq polymerase-derived products. However, there is no efficient method for the cloning...

EasyCloneYALI: CRISPR/Cas9-Based Synthetic Toolbox for Engineering of the Yeast Yarrowia lipolytica.

Biotechnology journal

Holkenbrink C, Dam MI, Kildegaard KR, Beder J, Dahlin J, Doménech Belda D, Borodina I.
PMID: 29377615
Biotechnol J. 2018 Sep;13(9):e1700543. doi: 10.1002/biot.201700543. Epub 2018 Feb 14.

The oleaginous yeast Yarrowia lipolytica is an emerging host for production of fatty acid-derived chemicals. To enable rapid iterative metabolic engineering of this yeast, there is a need for well-characterized genetic parts and convenient and reliable methods for their...

Showing 1 to 2 of 2 entries