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Improving flux predictions by integrating data from multiple strains.

Bioinformatics (Oxford, England)

Long MR, Reed JL.
PMID: 27998937
Bioinformatics. 2017 Mar 15;33(6):893-900. doi: 10.1093/bioinformatics/btw706.

MOTIVATION: Incorporating experimental data into constraint-based models can improve the quality and accuracy of their metabolic flux predictions. Unfortunately, routinely and easily measured experimental data such as growth rates, extracellular fluxes, transcriptomics and even proteomics are not always sufficient...

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Long MR, Reed JL. Improving flux predictions by integrating data from multiple strains. Bioinformatics. 2017;33(6):893-900doi: 10.1093/bioinformatics/btw706.
Long, M. R., & Reed, J. L. (2017). Improving flux predictions by integrating data from multiple strains. Bioinformatics (Oxford, England), 33(6), 893-900. https://doi.org/10.1093/bioinformatics/btw706
Long, Matthew R, and Reed, Jennifer L. "Improving flux predictions by integrating data from multiple strains." Bioinformatics (Oxford, England) vol. 33,6 (2017): 893-900. doi: https://doi.org/10.1093/bioinformatics/btw706
Long MR, Reed JL. Improving flux predictions by integrating data from multiple strains. Bioinformatics. 2017 Mar 15;33(6):893-900. doi: 10.1093/bioinformatics/btw706. PMID: 27998937.
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CRISPR/Cas9 facilitates genomic editing for large-scale functional studies in pluripotent stem cell cultures.

Human genetics

Li XF, Zhou YW, Cai PF, Fu WC, Wang JH, Chen JY, Yang QN.
PMID: 31606751
Hum Genet. 2019 Dec;138(11):1217-1225. doi: 10.1007/s00439-019-02071-z. Epub 2019 Oct 12.

Pluripotent stem cell (PSC) cultures form an integral part of biomedical and medical research due to their capacity to rapidly proliferate and differentiate into hundreds of highly specialized cell types. This makes them a highly useful tool in exploring...

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Li XF, Zhou YW, Cai PF, et al. CRISPR/Cas9 facilitates genomic editing for large-scale functional studies in pluripotent stem cell cultures. Hum Genet. 2019;138(11):1217-1225doi: 10.1007/s00439-019-02071-z.
Li, X. F., Zhou, Y. W., Cai, P. F., Fu, W. C., Wang, J. H., Chen, J. Y., & Yang, Q. N. (2019). CRISPR/Cas9 facilitates genomic editing for large-scale functional studies in pluripotent stem cell cultures. Human genetics, 138(11), 1217-1225. https://doi.org/10.1007/s00439-019-02071-z
Li, Xiao-Fei, et al. "CRISPR/Cas9 facilitates genomic editing for large-scale functional studies in pluripotent stem cell cultures." Human genetics vol. 138,11 (2019): 1217-1225. doi: https://doi.org/10.1007/s00439-019-02071-z
Li XF, Zhou YW, Cai PF, Fu WC, Wang JH, Chen JY, Yang QN. CRISPR/Cas9 facilitates genomic editing for large-scale functional studies in pluripotent stem cell cultures. Hum Genet. 2019 Dec;138(11):1217-1225. doi: 10.1007/s00439-019-02071-z. Epub 2019 Oct 12. PMID: 31606751.
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RNAi/CRISPR Screens: from a Pool to a Valid Hit.

Trends in biotechnology

Schuster A, Erasimus H, Fritah S, Nazarov PV, van Dyck E, Niclou SP, Golebiewska A.
PMID: 30177380
Trends Biotechnol. 2019 Jan;37(1):38-55. doi: 10.1016/j.tibtech.2018.08.002. Epub 2018 Aug 31.

High-throughput genetic screens interfering with gene expression are invaluable tools to identify gene function and phenotype-to-genotype interactions. Implementing such screens in the laboratory is challenging, and the choice between currently available technologies based on RNAi and CRISPR/Cas9 (CRISPR-associated protein...

Cite
Schuster A, Erasimus H, Fritah S, et al. RNAi/CRISPR Screens: from a Pool to a Valid Hit. Trends Biotechnol. 2018;37(1):38-55doi: 10.1016/j.tibtech.2018.08.002.
Schuster, A., Erasimus, H., Fritah, S., Nazarov, P. V., van Dyck, E., Niclou, S. P., & Golebiewska, A. (2019). RNAi/CRISPR Screens: from a Pool to a Valid Hit. Trends in biotechnology, 37(1), 38-55. https://doi.org/10.1016/j.tibtech.2018.08.002
Schuster, Anne, et al. "RNAi/CRISPR Screens: from a Pool to a Valid Hit." Trends in biotechnology vol. 37,1 (2019): 38-55. doi: https://doi.org/10.1016/j.tibtech.2018.08.002
Schuster A, Erasimus H, Fritah S, Nazarov PV, van Dyck E, Niclou SP, Golebiewska A. RNAi/CRISPR Screens: from a Pool to a Valid Hit. Trends Biotechnol. 2019 Jan;37(1):38-55. doi: 10.1016/j.tibtech.2018.08.002. Epub 2018 Aug 31. PMID: 30177380.
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