Display options
Cite
Li XF, Zhou YW, Cai PF, et al. CRISPR/Cas9 facilitates genomic editing for large-scale functional studies in pluripotent stem cell cultures. Hum Genet. 2019;138(11):1217-1225doi: 10.1007/s00439-019-02071-z.
Li, X. F., Zhou, Y. W., Cai, P. F., Fu, W. C., Wang, J. H., Chen, J. Y., & Yang, Q. N. (2019). CRISPR/Cas9 facilitates genomic editing for large-scale functional studies in pluripotent stem cell cultures. Human genetics, 138(11), 1217-1225. https://doi.org/10.1007/s00439-019-02071-z
Li, Xiao-Fei, et al. "CRISPR/Cas9 facilitates genomic editing for large-scale functional studies in pluripotent stem cell cultures." Human genetics vol. 138,11 (2019): 1217-1225. doi: https://doi.org/10.1007/s00439-019-02071-z
Li XF, Zhou YW, Cai PF, Fu WC, Wang JH, Chen JY, Yang QN. CRISPR/Cas9 facilitates genomic editing for large-scale functional studies in pluripotent stem cell cultures. Hum Genet. 2019 Dec;138(11):1217-1225. doi: 10.1007/s00439-019-02071-z. Epub 2019 Oct 12. PMID: 31606751.
Copy Download .nbib Format: NLM
Share it on

Hum Genet. 2019 Dec;138(11):1217-1225. doi: 10.1007/s00439-019-02071-z. Epub 2019 Oct 12.

CRISPR/Cas9 facilitates genomic editing for large-scale functional studies in pluripotent stem cell cultures.

Human genetics

Xiao-Fei Li, Yong-Wei Zhou, Peng-Fei Cai, Wei-Cong Fu, Jin-Hua Wang, Jin-Yang Chen, Qi-Ning Yang

Affiliations

  1. Department of Joint Surgery, Jinhua Municipal Central Hospital, No. 365 Renmin East Road, Wucheng District, Jinhua, 321000, Zhejiang, People's Republic of China.
  2. Research and Development Department, Zhejiang Healthfuture Institute for Cell-Based Applied Technology, Hangzhou, 310052, Zhejiang, People's Republic of China.
  3. Department of Joint Surgery, Jinhua Municipal Central Hospital, No. 365 Renmin East Road, Wucheng District, Jinhua, 321000, Zhejiang, People's Republic of China. [email protected].

PMID: 31606751 DOI: 10.1007/s00439-019-02071-z

Abstract

Pluripotent stem cell (PSC) cultures form an integral part of biomedical and medical research due to their capacity to rapidly proliferate and differentiate into hundreds of highly specialized cell types. This makes them a highly useful tool in exploring human physiology and disease. Genomic editing of PSC cultures is an essential method of attaining answers to basic physiological functions, developing in vitro models of human disease, and exploring potential therapeutic strategies and the identification of drug targets. Achieving reliable and efficient genomic editing is an important aspect of using large-scale PSC cultures. The CRISPR/Cas9 genomic editing tool has facilitated highly efficient gene knockout, gene correction, or gene modifications through the design and use of single-guide RNAs which are delivered to the target DNA via Cas9. CRISPR/Cas9 modification of PSCs has furthered the understanding of basic physiology and has been utilized to develop in vitro disease models, to test therapeutic strategies, and to facilitate regenerative or tissue repair approaches. In this review, we discuss the benefits of the CRISPR/Cas9 system in large-scale PSC cultures.

References

  1. Trends Microbiol. 2016 Oct;24(10):811-820 - PubMed
  2. Stem Cell Res. 2016 Mar;16(2):377-86 - PubMed
  3. Mol Ther Nucleic Acids. 2016 Jan 05;5:e273 - PubMed
  4. Proc Natl Acad Sci U S A. 1992 May 15;89(10):4275-9 - PubMed
  5. Biochimie. 2015 Oct;117:119-28 - PubMed
  6. Nucleic Acids Res. 2000 Sep 1;28(17):3361-9 - PubMed
  7. Cell Stem Cell. 2014 Dec 4;15(6):707-19 - PubMed
  8. Cell Calcium. 2018 Jul;73:104-111 - PubMed
  9. Nat Rev Genet. 2014 Sep;15(9):625-39 - PubMed
  10. Cell Stem Cell. 2014 Jan 2;14(1):13-26 - PubMed
  11. Mol Genet Metab. 2019 Jun;127(2):158-165 - PubMed
  12. Genetics. 2011 Aug;188(4):773-82 - PubMed
  13. Nat Biotechnol. 2016 Sep 08;34(9):933-941 - PubMed
  14. Hum Mol Genet. 2014 Sep 15;23(R1):R40-6 - PubMed
  15. J Biotechnol. 2015 Oct 10;211:56-65 - PubMed
  16. Drug Discov Today. 2019 Apr;24(4):955-970 - PubMed
  17. Cell Stem Cell. 2017 Oct 5;21(4):472-488.e10 - PubMed
  18. J Pharm Bioallied Sci. 2014 Jan;6(1):2-9 - PubMed
  19. Cell Stem Cell. 2013 Jun 6;12(6):669-77 - PubMed
  20. Int J Mol Sci. 2018 Dec 19;19(12): - PubMed
  21. FEBS J. 2016 Sep;283(17):3232-8 - PubMed
  22. Nature. 2014 Sep 25;513(7519):569-73 - PubMed
  23. Science. 2014 Jan 3;343(6166):84-87 - PubMed
  24. Stem Cells Int. 2017;2017:8960236 - PubMed
  25. Cell. 2006 Aug 25;126(4):663-76 - PubMed
  26. Nat Biotechnol. 2007 Jul;25(7):778-85 - PubMed
  27. Nat Rev Genet. 2010 Sep;11(9):636-46 - PubMed
  28. Genetics. 2010 Oct;186(2):757-61 - PubMed
  29. Science. 1998 Nov 6;282(5391):1145-7 - PubMed
  30. Cell Stem Cell. 2016 Apr 7;18(4):541-53 - PubMed
  31. Nat Biotechnol. 2012 May;30(5):460-5 - PubMed
  32. Cell Stem Cell. 2017 Jan 5;20(1):70-86 - PubMed
  33. Biotechnol J. 2015 Feb;10(2):258-72 - PubMed
  34. Proc Natl Acad Sci U S A. 2012 Sep 25;109(39):E2579-86 - PubMed
  35. Sci Rep. 2015 Jul 03;5:11791 - PubMed
  36. Cell Stem Cell. 2015 Aug 6;17(2):233-44 - PubMed
  37. Nat Commun. 2019 Jan 3;10(1):53 - PubMed
  38. Stem Cell Reports. 2013 Sep 26;1(4):283-92 - PubMed
  39. Hum Gene Ther. 2018 Mar;29(3):366-380 - PubMed
  40. PLoS Genet. 2013 Apr;9(4):e1003454 - PubMed
  41. Nat Methods. 2011 Jan;8(1):74-9 - PubMed
  42. Nat Biotechnol. 2000 Apr;18(4):399-404 - PubMed
  43. Nat Rev Mol Cell Biol. 2013 Jan;14(1):49-55 - PubMed
  44. Am J Hum Genet. 2017 Aug 3;101(2):167-176 - PubMed
  45. Proc Natl Acad Sci U S A. 1996 Feb 6;93(3):1156-60 - PubMed
  46. J Exp Clin Cancer Res. 2018 Mar 20;37(1):62 - PubMed
  47. Microbiology. 2009 Mar;155(Pt 3):733-740 - PubMed
  48. Cell Stem Cell. 2012 Jun 14;10(6):740-749 - PubMed
  49. Biores Open Access. 2016 May 01;5(1):127-36 - PubMed
  50. Brain Res. 2016 May 1;1638(Pt A):30-41 - PubMed
  51. Int J Stem Cells. 2019 Mar 30;12(1):114-124 - PubMed
  52. Curr Opin Immunol. 2015 Feb;32:36-41 - PubMed
  53. Mol Cell. 2008 Jul 25;31(2):294-301 - PubMed
  54. Biol Direct. 2006 Mar 16;1:7 - PubMed
  55. Nat Med. 2015 Feb;21(2):121-31 - PubMed
  56. Stem Cell Res. 2019 Jan;34:101378 - PubMed
  57. Cell Stem Cell. 2015 Jul 2;17(1):11-22 - PubMed
  58. Science. 2008 Aug 15;321(5891):960-4 - PubMed
  59. Nucleic Acids Res. 2016 Nov 2;44(19):e149 - PubMed
  60. Cell Stem Cell. 2013 Feb 7;12(2):238-51 - PubMed
  61. GM Crops Food. 2015;6(4):266-76 - PubMed
  62. Stem Cell Res Ther. 2018 Jan 12;9(1):8 - PubMed
  63. Nature. 2010 Nov 4;468(7320):67-71 - PubMed
  64. Genome Biol. 2015 Nov 09;16:247 - PubMed
  65. Arch Immunol Ther Exp (Warsz). 2017 Jun;65(3):233-240 - PubMed
  66. J Parkinsons Dis. 2019;9(2):265-281 - PubMed
  67. Science. 2012 Aug 17;337(6096):816-21 - PubMed
  68. Methods. 2019 Jul 15;164-165:109-119 - PubMed
  69. Mol Ther. 2008 Feb;16(2):352-8 - PubMed
  70. Nat Biotechnol. 2015 Sep;33(9):985-989 - PubMed
  71. Nat Methods. 2008 May;5(5):374-5 - PubMed
  72. Science. 2014 Nov 28;346(6213):1258096 - PubMed
  73. Science. 2013 Feb 15;339(6121):823-6 - PubMed
  74. Tissue Eng Part A. 2019 May;25(9-10):759-772 - PubMed
  75. Nat Protoc. 2013 Nov;8(11):2281-2308 - PubMed
  76. Gut. 2019 Apr;68(4):684-692 - PubMed
  77. Cell Stem Cell. 2013 Apr 4;12(4):393-4 - PubMed
  78. Mol Ther Nucleic Acids. 2017 Dec 15;9:230-241 - PubMed
  79. Colloids Surf B Biointerfaces. 2017 Nov 1;159:62-77 - PubMed
  80. Nat Rev Microbiol. 2011 Jun;9(6):467-77 - PubMed
  81. Cell Stem Cell. 2014 Nov 6;15(5):643-52 - PubMed
  82. Nat Commun. 2015 Oct 23;6:8715 - PubMed
  83. Protein Cell. 2020 Jan;11(1):1-22 - PubMed
  84. Biostatistics. 2019 Apr 1;20(2):273-286 - PubMed
  85. Nat Commun. 2019 Feb 25;10(1):928 - PubMed
  86. Elife. 2013 Jan 29;2:e00471 - PubMed
  87. Nat Biotechnol. 2011 Jul 07;29(8):731-4 - PubMed

MeSH terms

Publication Types

Grant support