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Showing 1 to 12 of 17 entries
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Preparation of iPSCs for Targeted Proteomic Analysis.

Methods in molecular biology (Clifton, N.J.)

Baud A, Heywood WE, Little D, Gissen P, Mills K.
PMID: 31124110
Methods Mol Biol. 2019;1994:131-139. doi: 10.1007/978-1-4939-9477-9_11.

Induced pluripotent stem cells have great potential as a human model system in regenerative medicine, disease modeling, and drug screening. However, extensive analysis of iPSC are required before their therapeutic applications. With recent developments in mass spectrometry and proteomics,...

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Baud A, Heywood WE, Little D, et al. Preparation of iPSCs for Targeted Proteomic Analysis. Methods Mol Biol. 2019;1994:131-139doi: 10.1007/978-1-4939-9477-9_11.
Baud, A., Heywood, W. E., Little, D., Gissen, P., & Mills, K. (2019). Preparation of iPSCs for Targeted Proteomic Analysis. Methods in molecular biology (Clifton, N.J.), 1994131-139. https://doi.org/10.1007/978-1-4939-9477-9_11
Baud, Anna, et al. "Preparation of iPSCs for Targeted Proteomic Analysis." Methods in molecular biology (Clifton, N.J.) vol. 1994 (2019): 131-139. doi: https://doi.org/10.1007/978-1-4939-9477-9_11
Baud A, Heywood WE, Little D, Gissen P, Mills K. Preparation of iPSCs for Targeted Proteomic Analysis. Methods Mol Biol. 2019;1994:131-139. doi: 10.1007/978-1-4939-9477-9_11. PMID: 31124110.
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High-Content Imaging to Phenotype Human Primary and iPSC-Derived Cells.

Methods in molecular biology (Clifton, N.J.)

Veschini L, Sailem H, Malani D, Pietiäinen V, Stojiljkovic A, Wiseman E, Danovi D.
PMID: 33165865
Methods Mol Biol. 2021;2185:423-445. doi: 10.1007/978-1-0716-0810-4_27.

Increasingly powerful microscopy, liquid handling, and computational techniques have enabled cell imaging in high throughput. Microscopy images are quantified using high-content analysis platforms linking object features to cell behavior. This can be attempted on physiologically relevant cell models, including...

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Veschini L, Sailem H, Malani D, et al. High-Content Imaging to Phenotype Human Primary and iPSC-Derived Cells. Methods Mol Biol. 2021;2185:423-445doi: 10.1007/978-1-0716-0810-4_27.
Veschini, L., Sailem, H., Malani, D., Pietiäinen, V., Stojiljkovic, A., Wiseman, E., & Danovi, D. (2021). High-Content Imaging to Phenotype Human Primary and iPSC-Derived Cells. Methods in molecular biology (Clifton, N.J.), 2185423-445. https://doi.org/10.1007/978-1-0716-0810-4_27
Veschini, Lorenzo, et al. "High-Content Imaging to Phenotype Human Primary and iPSC-Derived Cells." Methods in molecular biology (Clifton, N.J.) vol. 2185 (2021): 423-445. doi: https://doi.org/10.1007/978-1-0716-0810-4_27
Veschini L, Sailem H, Malani D, Pietiäinen V, Stojiljkovic A, Wiseman E, Danovi D. High-Content Imaging to Phenotype Human Primary and iPSC-Derived Cells. Methods Mol Biol. 2021;2185:423-445. doi: 10.1007/978-1-0716-0810-4_27. PMID: 33165865.
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The proteomes of native and induced pluripotent stem cells.

Nature methods

Pera MF.
PMID: 21959134
Nat Methods. 2011 Sep 29;8(10):807-8. doi: 10.1038/nmeth.1707.

No abstract available.

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Pera MF. The proteomes of native and induced pluripotent stem cells. Nat Methods. 2011;8(10):807-8doi: 10.1038/nmeth.1707.
Pera, M. F. (2011). The proteomes of native and induced pluripotent stem cells. Nature methods, 8(10), 807-8. https://doi.org/10.1038/nmeth.1707
Pera, Martin F. "The proteomes of native and induced pluripotent stem cells." Nature methods vol. 8,10 (2011): 807-8. doi: https://doi.org/10.1038/nmeth.1707
Pera MF. The proteomes of native and induced pluripotent stem cells. Nat Methods. 2011 Sep 29;8(10):807-8. doi: 10.1038/nmeth.1707. PMID: 21959134.
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Array-comparative genomic hybridization characterization of human pluripotent stem cells.

Methods in molecular biology (Clifton, N.J.)

Elliott AM, Elliott KA, Kammesheidt A.
PMID: 22528361
Methods Mol Biol. 2012;873:261-7. doi: 10.1007/978-1-61779-794-1_17.

During culture adaptation, human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) tend to acquire chromosomal aberrations. Generally, stem cell lines are screened for large-scale chromosomal changes using low resolution karyotype analysis. Recent studies characterizing human stem...

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Elliott AM, Elliott KA, Kammesheidt A. Array-comparative genomic hybridization characterization of human pluripotent stem cells. Methods Mol Biol. 2012;873:261-7doi: 10.1007/978-1-61779-794-1_17.
Elliott, A. M., Elliott, K. A., & Kammesheidt, A. (2012). Array-comparative genomic hybridization characterization of human pluripotent stem cells. Methods in molecular biology (Clifton, N.J.), 873261-7. https://doi.org/10.1007/978-1-61779-794-1_17
Elliott, Aaron M, et al. "Array-comparative genomic hybridization characterization of human pluripotent stem cells." Methods in molecular biology (Clifton, N.J.) vol. 873 (2012): 261-7. doi: https://doi.org/10.1007/978-1-61779-794-1_17
Elliott AM, Elliott KA, Kammesheidt A. Array-comparative genomic hybridization characterization of human pluripotent stem cells. Methods Mol Biol. 2012;873:261-7. doi: 10.1007/978-1-61779-794-1_17. PMID: 22528361.
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iPSCs: Unstable origins?.

Molecular therapy : the journal of the American Society of Gene Therapy

Mukherjee S, Thrasher AJ.
PMID: 21720379
Mol Ther. 2011 Jul;19(7):1188-90. doi: 10.1038/mt.2011.116.

No abstract available.

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Mukherjee S, Thrasher AJ. iPSCs: Unstable origins?. Mol Ther. 2011;19(7):1188-90doi: 10.1038/mt.2011.116.
Mukherjee, S., & Thrasher, A. J. (2011). iPSCs: Unstable origins?. Molecular therapy : the journal of the American Society of Gene Therapy, 19(7), 1188-90. https://doi.org/10.1038/mt.2011.116
Mukherjee, Sayandip, and Thrasher, Adrian J. "iPSCs: Unstable origins?." Molecular therapy : the journal of the American Society of Gene Therapy vol. 19,7 (2011): 1188-90. doi: https://doi.org/10.1038/mt.2011.116
Mukherjee S, Thrasher AJ. iPSCs: Unstable origins?. Mol Ther. 2011 Jul;19(7):1188-90. doi: 10.1038/mt.2011.116. PMID: 21720379; PMCID: PMC3129555.
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Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9.

Journal of visualized experiments : JoVE

Haupt A, Grancharova T, Arakaki J, Fuqua MA, Roberts B, Gunawardane RN.
PMID: 30199041
J Vis Exp. 2018 Aug 25;(138). doi: 10.3791/58130.

A protocol is presented for generating human induced pluripotent stem cells (hiPSCs) that express endogenous proteins fused to in-frame N- or C-terminal fluorescent tags. The prokaryotic CRISPR/Cas9 system (clustered regularly interspaced short palindromic repeats/CRISPR-associated 9) may be used to...

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Haupt A, Grancharova T, Arakaki J, et al. Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9. J Vis Exp. 2018;(138)doi: 10.3791/58130.
Haupt, A., Grancharova, T., Arakaki, J., Fuqua, M. A., Roberts, B., & Gunawardane, R. N. (2018). Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9. Journal of visualized experiments : JoVE, (138), . https://doi.org/10.3791/58130
Haupt, Amanda, et al. "Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9." Journal of visualized experiments : JoVE vol. ,138 (2018). doi: https://doi.org/10.3791/58130
Haupt A, Grancharova T, Arakaki J, Fuqua MA, Roberts B, Gunawardane RN. Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9. J Vis Exp. 2018 Aug 25;(138). doi: 10.3791/58130. PMID: 30199041; PMCID: PMC6231893.
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Combining hiPSCs and Human Genetics: Major Applications in Drug Development.

Cell stem cell

Zhang J, Li H, Trounson A, Wu JC, Nioi P.
PMID: 28777942
Cell Stem Cell. 2017 Aug 03;21(2):161-165. doi: 10.1016/j.stem.2017.07.012.

Merging iPSC models and human genetic research has opened up new avenues in understanding disease mechanisms and target biology, which facilitate exciting translation of this research to many areas of drug development. We highlight recent applications of these combined...

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Zhang J, Li H, Trounson A, et al. Combining hiPSCs and Human Genetics: Major Applications in Drug Development. Cell Stem Cell. 2017;21(2):161-165doi: 10.1016/j.stem.2017.07.012.
Zhang, J., Li, H., Trounson, A., Wu, J. C., & Nioi, P. (2017). Combining hiPSCs and Human Genetics: Major Applications in Drug Development. Cell stem cell, 21(2), 161-165. https://doi.org/10.1016/j.stem.2017.07.012
Zhang, Jin, et al. "Combining hiPSCs and Human Genetics: Major Applications in Drug Development." Cell stem cell vol. 21,2 (2017): 161-165. doi: https://doi.org/10.1016/j.stem.2017.07.012
Zhang J, Li H, Trounson A, Wu JC, Nioi P. Combining hiPSCs and Human Genetics: Major Applications in Drug Development. Cell Stem Cell. 2017 Aug 03;21(2):161-165. doi: 10.1016/j.stem.2017.07.012. PMID: 28777942.
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Taming Human Genetic Variability: Transcriptomic Meta-Analysis Guides the Experimental Design and Interpretation of iPSC-Based Disease Modeling.

Stem cell reports

Germain PL, Testa G.
PMID: 28591656
Stem Cell Reports. 2017 Jun 06;8(6):1784-1796. doi: 10.1016/j.stemcr.2017.05.012.

Both the promises and pitfalls of the cell reprogramming research platform rest on human genetic variation, making the measurement of its impact one of the most urgent issues in the field. Harnessing large transcriptomics datasets of induced pluripotent stem...

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Germain PL, Testa G. Taming Human Genetic Variability: Transcriptomic Meta-Analysis Guides the Experimental Design and Interpretation of iPSC-Based Disease Modeling. Stem Cell Reports. 2017;8(6):1784-1796doi: 10.1016/j.stemcr.2017.05.012.
Germain, P. L., & Testa, G. (2017). Taming Human Genetic Variability: Transcriptomic Meta-Analysis Guides the Experimental Design and Interpretation of iPSC-Based Disease Modeling. Stem cell reports, 8(6), 1784-1796. https://doi.org/10.1016/j.stemcr.2017.05.012
Germain, Pierre-Luc, and Testa, Giuseppe. "Taming Human Genetic Variability: Transcriptomic Meta-Analysis Guides the Experimental Design and Interpretation of iPSC-Based Disease Modeling." Stem cell reports vol. 8,6 (2017): 1784-1796. doi: https://doi.org/10.1016/j.stemcr.2017.05.012
Germain PL, Testa G. Taming Human Genetic Variability: Transcriptomic Meta-Analysis Guides the Experimental Design and Interpretation of iPSC-Based Disease Modeling. Stem Cell Reports. 2017 Jun 06;8(6):1784-1796. doi: 10.1016/j.stemcr.2017.05.012. PMID: 28591656; PMCID: PMC5470233.
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Systems-Wide Approaches in Induced Pluripotent Stem Cell Models.

Annual review of pathology

Lau E, Paik DT, Wu JC.
PMID: 30379619
Annu Rev Pathol. 2019 Jan 24;14:395-419. doi: 10.1146/annurev-pathmechdis-012418-013046. Epub 2018 Oct 31.

Human induced pluripotent stem cells (iPSCs) provide a renewable supply of patient-specific and tissue-specific cells for cellular and molecular studies of disease mechanisms. Combined with advances in various omics technologies, iPSC models can be used to profile the expression...

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Lau E, Paik DT, Wu JC. Systems-Wide Approaches in Induced Pluripotent Stem Cell Models. Annu Rev Pathol. 2018;14:395-419doi: 10.1146/annurev-pathmechdis-012418-013046.
Lau, E., Paik, D. T., & Wu, J. C. (2019). Systems-Wide Approaches in Induced Pluripotent Stem Cell Models. Annual review of pathology, 14395-419. https://doi.org/10.1146/annurev-pathmechdis-012418-013046
Lau, Edward, et al. "Systems-Wide Approaches in Induced Pluripotent Stem Cell Models." Annual review of pathology vol. 14 (2019): 395-419. doi: https://doi.org/10.1146/annurev-pathmechdis-012418-013046
Lau E, Paik DT, Wu JC. Systems-Wide Approaches in Induced Pluripotent Stem Cell Models. Annu Rev Pathol. 2019 Jan 24;14:395-419. doi: 10.1146/annurev-pathmechdis-012418-013046. Epub 2018 Oct 31. PMID: 30379619; PMCID: PMC6450651.
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Key regulations related to stem cell research.

Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie

Aluaş M, Todea DA, Gherman CD.
PMID: 31658351
Rom J Morphol Embryol. 2019;60(2):723-728.

Medicine is an ever-changing science. Stem cell research is nowadays part of the medicine. After developments and trials for decades, in 1988 it was announced that a variety of diseases and injuries would be cured with new stem cell...

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Aluaş M, Todea DA, Gherman CD. Key regulations related to stem cell research. Rom J Morphol Embryol. 2019;60(2):723-728
Aluaş, M., Todea, D. A., & Gherman, C. D. (2019). Key regulations related to stem cell research. Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie, 60(2), 723-728.
Aluaş, Maria, et al. "Key regulations related to stem cell research." Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie vol. 60,2 (2019): 723-728.
Aluaş M, Todea DA, Gherman CD. Key regulations related to stem cell research. Rom J Morphol Embryol. 2019;60(2):723-728. PMID: 31658351.
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Profiling of naïve and primed human pluripotent stem cells reveals state-associated miRNAs.

Scientific reports

Dodsworth BT, Hatje K, Rostovskaya M, Flynn R, Meyer CA, Cowley SA.
PMID: 32601281
Sci Rep. 2020 Jun 29;10(1):10542. doi: 10.1038/s41598-020-67376-w.

Naïve human pluripotent stem cells (hPSC) resemble the embryonic epiblast at an earlier time-point in development than conventional, 'primed' hPSC. We present a comprehensive miRNA profiling of naïve-to-primed transition in hPSC, a process recapitulating aspects of early in vivo...

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Dodsworth BT, Hatje K, Rostovskaya M, et al. Profiling of naïve and primed human pluripotent stem cells reveals state-associated miRNAs. Sci Rep. 2020;10(1):10542doi: 10.1038/s41598-020-67376-w.
Dodsworth, B. T., Hatje, K., Rostovskaya, M., Flynn, R., Meyer, C. A., & Cowley, S. A. (2020). Profiling of naïve and primed human pluripotent stem cells reveals state-associated miRNAs. Scientific reports, 10(1), 10542. https://doi.org/10.1038/s41598-020-67376-w
Dodsworth, Benjamin T, et al. "Profiling of naïve and primed human pluripotent stem cells reveals state-associated miRNAs." Scientific reports vol. 10,1 (2020): 10542. doi: https://doi.org/10.1038/s41598-020-67376-w
Dodsworth BT, Hatje K, Rostovskaya M, Flynn R, Meyer CA, Cowley SA. Profiling of naïve and primed human pluripotent stem cells reveals state-associated miRNAs. Sci Rep. 2020 Jun 29;10(1):10542. doi: 10.1038/s41598-020-67376-w. PMID: 32601281; PMCID: PMC7324611.
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Towards chamber specific heart-on-a-chip for drug testing applications.

Advanced drug delivery reviews

Zhao Y, Rafatian N, Wang EY, Wu Q, Lai BFL, Lu RX, Savoji H, Radisic M.
PMID: 31917972
Adv Drug Deliv Rev. 2020;165:60-76. doi: 10.1016/j.addr.2019.12.002. Epub 2020 Jan 07.

Modeling of human organs has long been a task for scientists in order to lower the costs of therapeutic development and understand the pathological onset of human disease. For decades, despite marked differences in genetics and etiology, animal models...

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Zhao Y, Rafatian N, Wang EY, et al. Towards chamber specific heart-on-a-chip for drug testing applications. Adv Drug Deliv Rev. 2020;165:60-76doi: 10.1016/j.addr.2019.12.002.
Zhao, Y., Rafatian, N., Wang, E. Y., Wu, Q., Lai, B. F. L., Lu, R. X., Savoji, H., & Radisic, M. (2020). Towards chamber specific heart-on-a-chip for drug testing applications. Advanced drug delivery reviews, 16560-76. https://doi.org/10.1016/j.addr.2019.12.002
Zhao, Yimu, et al. "Towards chamber specific heart-on-a-chip for drug testing applications." Advanced drug delivery reviews vol. 165 (2020): 60-76. doi: https://doi.org/10.1016/j.addr.2019.12.002
Zhao Y, Rafatian N, Wang EY, Wu Q, Lai BFL, Lu RX, Savoji H, Radisic M. Towards chamber specific heart-on-a-chip for drug testing applications. Adv Drug Deliv Rev. 2020;165:60-76. doi: 10.1016/j.addr.2019.12.002. Epub 2020 Jan 07. PMID: 31917972; PMCID: PMC7338250.
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Showing 1 to 12 of 17 entries