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Elliott AM, Elliott KA, Kammesheidt A. Array-comparative genomic hybridization characterization of human pluripotent stem cells. Methods Mol Biol. 2012;873:261-7doi: 10.1007/978-1-61779-794-1_17.
Elliott, A. M., Elliott, K. A., & Kammesheidt, A. (2012). Array-comparative genomic hybridization characterization of human pluripotent stem cells. Methods in molecular biology (Clifton, N.J.), 873261-7. https://doi.org/10.1007/978-1-61779-794-1_17
Elliott, Aaron M, et al. "Array-comparative genomic hybridization characterization of human pluripotent stem cells." Methods in molecular biology (Clifton, N.J.) vol. 873 (2012): 261-7. doi: https://doi.org/10.1007/978-1-61779-794-1_17
Elliott AM, Elliott KA, Kammesheidt A. Array-comparative genomic hybridization characterization of human pluripotent stem cells. Methods Mol Biol. 2012;873:261-7. doi: 10.1007/978-1-61779-794-1_17. PMID: 22528361.
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Methods Mol Biol. 2012;873:261-7. doi: 10.1007/978-1-61779-794-1_17.

Array-comparative genomic hybridization characterization of human pluripotent stem cells.

Methods in molecular biology (Clifton, N.J.)

Aaron M Elliott, Kristi A Hohenstein Elliott, Anja Kammesheidt

Affiliations

  1. Ambry Genetics, Aliso Viejo, CA, USA. [email protected]

PMID: 22528361 DOI: 10.1007/978-1-61779-794-1_17

Abstract

During culture adaptation, human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) tend to acquire chromosomal aberrations. Generally, stem cell lines are screened for large-scale chromosomal changes using low resolution karyotype analysis. Recent studies characterizing human stem cells using array-comparative genomic hybridization (aCGH) suggests most abnormalities acquired during culture are under the resolution of karyotype analysis and therefore are routinely missed. Here, we describe a custom-designed stem cell focused microarray utilizing 44K probes, with increased resolution in relevant stem cell-associated and cancer-related genes.

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