Spectrochim Acta A Mol Biomol Spectrosc. 2000 Jun;56(7):1433-41. doi: 10.1016/s1386-1425(00)00245-6.

Characterization of the fluorescence emission properties of prodan in different reverse micellar environments.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy

B Sengupta, J Guharay, P K Sengupta

PMID: 10888447 DOI: 10.1016/s1386-1425(00)00245-6

Abstract

We have examined the steady state and time resolved fluorescence emission properties of the hydrophobic fluorescence probe, prodan, in three representative reverse micellar systems formed by the surfactants poly(oxyethylene) (tetramethylbutyl) phenylether (Triton X-100, neutral), cetyl trimethylammonium bromide (CTAB, cationic) and sodium bis-(2-ethylhexyl) sulfosuccinate (AOT, anionic) in organic solvent media containing different concentrations of water. The results obtained from the experiments indicate conspicuous dependence of the emission behaviour of prodan on the type of surfactant used and the water/surfactant molar ratio (w0). The nature of the emission profiles, along with relevant parameters namely emission maximum (lambda(em)max), anisotropy (r) and lifetime (tau) data are used to infer the distribution and microenvironments of the prodan molecules in the reverse micelles at different w0 values. Furthermore, quantitative estimates have been obtained for the polarities (in terms of the empirical polarity parameter E(T)(30)) of the sites of solubilization of the fluorophore in different reverse micellar systems.

Substances

• Fluorescent Dyes
• prodan
• 2-Naphthylamine

MeSH terms

• 2-Naphthylamine / analogs & derivatives
• 2-Naphthylamine / chemistry
• Fluorescence
• Fluorescent Dyes / chemistry

Publication Types

• Journal Article
• Research Support, Non-U.S. Gov't