J Chromatogr A. 2000 Jul 21;886(1):1-7. doi: 10.1016/s0021-9673(00)00510-0.

Mobile-phase-viscosity dependence on DNA separation in slalom chromatography.

Journal of chromatography. A

E Peyrin, Y C Guillaume, C Grosset, A Ravel, A Villet, C Garrel, J Alary, A Favier

PMID: 10950270 DOI: 10.1016/s0021-9673(00)00510-0

Abstract

Slalom chromatography (SC) is an alternative chromatographic procedure for the separation of relatively large double-stranded DNA molecules and is based on a new principle. The retardation of the DNA fragments from the cleavage of the Lambda DNA by the KpnI restriction enzyme was studied using an acetonitrile-phosphate buffer as a mobile phase with various concentrations of viscosity modifier (i.e. glycerol) and a C1 column as a stationary phase. The DNA molecule retention was accurately described over the glycerol concentration range using a model previously established. It was shown that the eluent viscosity increase enhanced the slalom chromatographic capacity to separate the DNA fragments. A connection between SC and 'hydrodynamic chromatography' processes was predicted to link the two processes in a global separation mechanism based on a non-equilibrium principle.

Substances

• DNA

MeSH terms

• Chromatography, Liquid / methods
• DNA / isolation & purification

Publication Types

• Journal Article