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Showing 1 to 12 of 63 entries
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Rapid and sensitive profiling and quantification of the human cell line proteome by LC-MS/MS without prefractionation.

Proteomics

Yin X, Liu X, Zhang Y, Yan G, Wang F, Lu H, Shen H, Yang P.
PMID: 25044409
Proteomics. 2014 Sep;14(17):2008-16. doi: 10.1002/pmic.201300510. Epub 2014 Aug 13.

In this paper, we demonstrate a rapid and reproducible 1D LC-MS/MS workflow for fast quantitative proteomic research. We have optimized the LC-MS/MS conditions, including digestion and gradient conditions, sample loading amount, and MS parameter settings. As a result, we...

Curatr: a web application for creating, curating and sharing a mass spectral library.

Bioinformatics (Oxford, England)

Palmer A, Phapale P, Fay D, Alexandrov T.
PMID: 29253079
Bioinformatics. 2018 Apr 15;34(8):1436-1438. doi: 10.1093/bioinformatics/btx786.

SUMMARY: We have developed a web application curatr for the rapid generation of high quality mass spectral fragmentation libraries from liquid-chromatography mass spectrometry datasets. Curatr handles datasets from single or multiplexed standards and extracts chromatographic profiles and potential fragmentation...

pH-Gradient Liquid Chromatography: Fundamentals and Examples.

Journal of AOAC International

Kubik Ł, Wiczling P, Kaliszan R.
PMID: 28703096
J AOAC Int. 2017 Nov 01;100(6):1590-1598. doi: 10.5740/jaoacint.17-0205. Epub 2017 Jul 13.

In this paper, we acquaint the readers with the fundamentals of gradient separation, followed by the latest innovations in this field. We describe the principles of organic modifier- and pH-gradient elution emphasizing the differences and similarities with isocratic separation....

Characterization of stationary phases by a linear solvation energy relationship utilizing supercritical fluid chromatography.

Journal of separation science

Mitchell CR, Benz NJ, Zhang S.
PMID: 20730839
J Sep Sci. 2010 Oct;33(19):3060-7. doi: 10.1002/jssc.201000371.

Supercritical fluid chromatography was utilized in combination with the Abraham model of linear solvation energy relationship to characterize 11 different HPLC stationary phases. System constants were determined at one supercritical fluid chromatography condition for each stationary phase. The results...

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification.

Journal of visualized experiments : JoVE

High AA, Tan H, Pagala VR, Niu M, Cho JH, Wang X, Bai B, Peng J.
PMID: 29286450
J Vis Exp. 2017 Nov 15;(129). doi: 10.3791/56474.

Many exceptional advances have been made in mass spectrometry (MS)-based proteomics, with particular technical progress in liquid chromatography (LC) coupled to tandem mass spectrometry (LC-MS/MS) and isobaric labeling multiplexing capacity. Here, we introduce a deep-proteomics profiling protocol that combines...

Systematic evaluation of supported liquid extraction in reducing matrix effect and improving extraction efficiency in LC-MS/MS based bioanalysis for 10 model pharmaceutical compounds.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

Jiang H, Cao H, Zhang Y, Fast DM.
PMID: 22410088
J Chromatogr B Analyt Technol Biomed Life Sci. 2012 Apr 01;891:71-80. doi: 10.1016/j.jchromb.2012.02.031. Epub 2012 Feb 23.

In past a few years, there has been a large increase in the application of supported liquid extraction (SLE) for LC-MS/MS based bioanalysis due to its distinct practical advantage in reduced time cost, ease of operation and the feasibility...

Editorial.

Biomedical chromatography : BMC

[No authors listed]
PMID: 25366171
Biomed Chromatogr. 2015 May;29(5):641-2. doi: 10.1002/bmc.3379. Epub 2014 Nov 04.

No abstract available.

Theoretical study of incomplete sampling of the first dimension in comprehensive two-dimensional chromatography.

Journal of chromatography. A

Seeley JV.
PMID: 12198965
J Chromatogr A. 2002 Jul 12;962(1):21-7. doi: 10.1016/s0021-9673(02)00461-2.

The process of regularly transferring material from the primary column to the secondary column is critical in producing comprehensive two-dimensional separations. A series of calculations have been performed to determine how sampling period, duty cycle, and sampling phase affect...

Evolution of solid-phase microextraction technology.

Journal of chromatography. A

Lord H, Pawliszyn J.
PMID: 10941672
J Chromatogr A. 2000 Jul 14;885(1):153-93. doi: 10.1016/s0021-9673(00)00535-5.

The main objective of this contribution is to describe the development of the concepts, techniques and devices associated with solid-phase microextraction, as a response to the evolution of understanding of the fundamental principles behind this technique. The discussion begins...

Rapid identification and quantitation of compounds with forensic interest using fast liquid chromatography-ion trap mass spectrometry and library searching.

Journal of chromatography. A

Pihlainen K, Sippola E, Kostiainen R.
PMID: 12779222
J Chromatogr A. 2003 Apr 25;994(1):93-102. doi: 10.1016/s0021-9673(03)00318-2.

A fast liquid chromatography-electrospray tandem mass spectrometric (LC-ESI-MS-MS) method by using a monolithic column, gradient elution and ion trap mass spectrometer was developed for 14 forensically interesting and chemically different compounds. All compounds were eluted within 2.5 min and...

Mobile-phase-viscosity dependence on DNA separation in slalom chromatography.

Journal of chromatography. A

Peyrin E, Guillaume YC, Grosset C, Ravel A, Villet A, Garrel C, Alary J, Favier A.
PMID: 10950270
J Chromatogr A. 2000 Jul 21;886(1):1-7. doi: 10.1016/s0021-9673(00)00510-0.

Slalom chromatography (SC) is an alternative chromatographic procedure for the separation of relatively large double-stranded DNA molecules and is based on a new principle. The retardation of the DNA fragments from the cleavage of the Lambda DNA by the...

Numerical and experimental investigation of analyte breakthrough from sampling loops used for multi-dimensional liquid chromatography.

Journal of chromatography. A

Moussa A, Lauer T, Stoll D, Desmet G, Broeckhoven K, Dalziel J.
PMID: 32797812
J Chromatogr A. 2020 Aug 30;1626:461283. doi: 10.1016/j.chroma.2020.461283. Epub 2020 Jun 01.

Two-dimensional liquid chromatography is increasingly being used to address challenging separations in fields ranging from pharmaceutical analysis to the food industry. A significant impediment to development of more methods is the lack of a complete theoretical foundation upon which...

Showing 1 to 12 of 63 entries