J Chromatogr A. 2020 Aug 30;1626:461283. doi: 10.1016/j.chroma.2020.461283. Epub 2020 Jun 01.

Numerical and experimental investigation of analyte breakthrough from sampling loops used for multi-dimensional liquid chromatography.

Journal of chromatography. A

Ali Moussa, Thomas Lauer, Dwight Stoll, Gert Desmet, Ken Broeckhoven, Dalziel

PMID: 32797812 DOI: 10.1016/j.chroma.2020.461283

Abstract

Two-dimensional liquid chromatography is increasingly being used to address challenging separations in fields ranging from pharmaceutical analysis to the food industry. A significant impediment to development of more methods is the lack of a complete theoretical foundation upon which sound development decisions can be made. One parameter that is currently not fully understood is the extent of filling of sampling loops in the case where effluent from the first dimension separation is transferred to the second dimension separation through this type of open loop interface. This is a highly important parameter because it is connected to several other variables in a 2D-LC system, including the first dimension flow rate, the sampling (modulation) time, and the loop volume. In this study we have used both numerical simulation methods and experimental measurements to understand the extent to which sampling loops can be filled before a significant fraction of the analyte is lost from the end of the loop. Variables included in the study are the analyte diffusion coefficient (D

Copyright © 2020. Published by Elsevier B.V.

Keywords: Analyte loss curve; Breakthrough; Loop coiling; Loop filling; Numerical simulations; Two-dimensional liquid chromatography

MeSH terms

• Chromatography, Liquid / instrumentation
• Chromatography, Liquid / methods*
• Diffusion
• Models, Theoretical

Publication Types

• Journal Article