J Chromatogr B Analyt Technol Biomed Life Sci. 2002 Apr 25;770(1):53-61. doi: 10.1016/s1570-0232(01)00582-7.

Supercritical fluid chromatography coupled to electrospray mass spectrometry: a powerful tool for the analysis of chiral mixtures.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

Marco Garzotti, Mahmoud Hamdan

PMID: 12013244 DOI: 10.1016/s1570-0232(01)00582-7

Abstract

Supercritical fluid chromatography coupled to a hybrid mass spectrometer (Q-Tof2) equipped with electrospray ion source has been used to separate and characterise a wide range of pharmaceutical racemates. We have chosen diverse molecular structures to demonstrate the potential of such experimental arrangement for high throughput analyses. The use of three different chiral stationary phases and different pressure/temperature working conditions provided clear indications on how such a high throughput method can be developed. The use of mass spectrometry was found to be essential for an unambiguous assignment of the eluting components particularly in the case of complex mixtures. The direct coupling of both systems without the need for a special interface resulted in similar peak shapes and peak widths in the UV and total ion current (TIC) chromatograms.

MeSH terms

• Chromatography, Supercritical Fluid / methods
• Sensitivity and Specificity
• Spectrometry, Mass, Electrospray Ionization / methods

Publication Types

• Journal Article