Display options
Cite
Zhang XF, Ge ZL, Sun LY, et al. Cloning and Expression of the Gene Coding for the Fusion Protein rhGM-CSF/LIF. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 1997;29(5):425-431
Zhang, X. F., Ge, Z. L., Sun, L. Y., Ding, Q. M., Zhang, Q. W., Cao, H., & Yuan, L. Z. (1997). Cloning and Expression of the Gene Coding for the Fusion Protein rhGM-CSF/LIF. Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica, 29(5), 425-431.
Zhang, Xue-Feng, et al. "Cloning and Expression of the Gene Coding for the Fusion Protein rhGM-CSF/LIF." Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica vol. 29,5 (1997): 425-431.
Zhang XF, Ge ZL, Sun LY, Ding QM, Zhang QW, Cao H, Yuan LZ. Cloning and Expression of the Gene Coding for the Fusion Protein rhGM-CSF/LIF. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 1997;29(5):425-431. PMID: 12215780.
Copy Download .nbib Format: NLM
Share it on

Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 1997;29(5):425-431.

Cloning and Expression of the Gene Coding for the Fusion Protein rhGM-CSF/LIF.

Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica

Xue-Feng Zhang, Zhong-Liang Ge, Li-Ya Sun, Qing-Ming Ding, Qun-Wei Zhang, Hua Cao, Li-Zhen Yuan

Affiliations

  1. Beijing Institute of Radiation Medicine, Beijing 100850, China.

PMID: 12215780

Abstract

A fusion gene was constructed in a plasmid in which the coding regions of human GM_CSF and LIF cDNAs were connected by a synthetic linker sequence encoding a short peptide G-S-G-G-S through DNA recombinant techniques. It was then subcloned into the pBV220 expression vector, and expressed in E. coli after transformation and temperature induction. The expressed protein named as rhGM-LIF was confirmed by Western blot. After purification, the determination of activities showed that rhGM-LIF exhibited both GM-CSF and LIF activities.

Publication Types