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Itzhaki H, Davis JH, Borochov A, et al. Deuterium Magnetic Resonance Studies of Senescence-Related Changes in the Physical Properties of Rose Petal Membrane Lipids. Plant Physiol. 1995;108(3):1029-1033doi: 10.1104/pp.108.3.1029.
Itzhaki, H., Davis, J. H., Borochov, A., Mayak, S., & Pauls, K. P. (1995). Deuterium Magnetic Resonance Studies of Senescence-Related Changes in the Physical Properties of Rose Petal Membrane Lipids. Plant physiology, 108(3), 1029-1033. https://doi.org/10.1104/pp.108.3.1029
Itzhaki, H., et al. "Deuterium Magnetic Resonance Studies of Senescence-Related Changes in the Physical Properties of Rose Petal Membrane Lipids." Plant physiology vol. 108,3 (1995): 1029-1033. doi: https://doi.org/10.1104/pp.108.3.1029
Itzhaki H, Davis JH, Borochov A, Mayak S, Pauls KP. Deuterium Magnetic Resonance Studies of Senescence-Related Changes in the Physical Properties of Rose Petal Membrane Lipids. Plant Physiol. 1995 Jul;108(3):1029-1033. doi: 10.1104/pp.108.3.1029. PMID: 12228524; PMCID: PMC157453.
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Plant Physiol. 1995 Jul;108(3):1029-1033. doi: 10.1104/pp.108.3.1029.

Deuterium Magnetic Resonance Studies of Senescence-Related Changes in the Physical Properties of Rose Petal Membrane Lipids.

Plant physiology

H. Itzhaki, J. H. Davis, A. Borochov, S. Mayak, K. P. Pauls

Affiliations

  1. The Kennedy-Leigh Centre for Horticultural Research, Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot 76100, Israel (H.I., A.B., S.M.).

PMID: 12228524 PMCID: PMC157453 DOI: 10.1104/pp.108.3.1029

Abstract

The physical properties of membrane lipids in senescing rose (Rosa hybrida L., cv Mercedes) petals were studied by deuterium nuclear magnetic resonance (2H-NMR) and fluorescence depolarization. All of the 2H-NMR spectra arising from deuterated dimyristoylphosphatidylcholine mixed with whole-lipid extracts from membranes of petals of different ages had a shape that is characteristic of liquid-crystalline lipid at 30[deg]C. Arrhenius plots of the moments of the 2H spectra and fluorescence depolarization values measured from 1,6-diphenyl hexatriene-labeled rose petal membrane lipid samples indicated that membrane lipid order increased with decreasing temperature as well as with increasing age of the petals. The latter trend is explained by previously observed increases in fatty acid saturation and increases in the sterol-to-phospholipid ratio that occur in rose petals during senescence. The 2H-NMR spectra obtained at 0[deg]C also contained quadrupolar splitting lines from lipid in the gel phase, confirming the occurrence of this phase in membranes from this tissue.

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