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Martin TA, Harding KG, Jiang WG. Regulation of angiogenesis and endothelial cell motility by matrix-bound fibroblasts. Angiogenesis. 1999;3(1):69-76doi: 10.1023/a:1009004212357.
Martin, T. A., Harding, K. G., & Jiang, W. G. (1999). Regulation of angiogenesis and endothelial cell motility by matrix-bound fibroblasts. Angiogenesis, 3(1), 69-76. https://doi.org/10.1023/a:1009004212357
Martin, T A, et al. "Regulation of angiogenesis and endothelial cell motility by matrix-bound fibroblasts." Angiogenesis vol. 3,1 (1999): 69-76. doi: https://doi.org/10.1023/a:1009004212357
Martin TA, Harding KG, Jiang WG. Regulation of angiogenesis and endothelial cell motility by matrix-bound fibroblasts. Angiogenesis. 1999;3(1):69-76. doi: 10.1023/a:1009004212357. PMID: 14517446.
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Angiogenesis. 1999;3(1):69-76. doi: 10.1023/a:1009004212357.

Regulation of angiogenesis and endothelial cell motility by matrix-bound fibroblasts.

Angiogenesis

T A Martin, K G Harding, W G Jiang

Affiliations

  1. Wound Healing Research Unit, University of Wales College of Medicine, Cardiff, UK. [email protected]

PMID: 14517446 DOI: 10.1023/a:1009004212357

Abstract

This study sought to examine the effect of matrix-bound fibroblasts on angiogenesis and endothelial cell motility. Promotion of angiogenesis by matrix-bound fibroblasts was assessed using rat aortic ring and endothelial tube formation assays. Enhancement of human endothelial cell motility by matrix-bound fibroblasts was assessed using cytodex-2 bead and colloidal gold phagokinetic motility assays. Antibody to hepatocyte growth factor/scatter factor (HGF/SF) but not vascular endothelial growth factor (VEGf) decreased fibroblast-enhanced motility of endothelial cells. The promotion of tube formation by matrix-bound fibroblasts was neutralised with antibodies to HGF/SF and VEGf, both known promoters of angiogenesis. HGF/SF presence was detected by ELISA; whilst the presence of VEGf was detected by Western blotting. These data show that matrix-embedded fibroblasts regulate the motility of vascular endothelial cells and enhance angiogenesis, an effect partly attributed to production of angiogenesis-promoting cytokines HGF/SF and/or VEGf.

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