J Chromatogr A. 2004 Mar 19;1030(1):149-55. doi: 10.1016/j.chroma.2003.12.033.

Rapid method for evaluating reversed-phase high-performance liquid chromatography column stability.

Journal of chromatography. A

Dania A Fonseca, Humberto R Gutiérrez, Kenneth E Collins, Carol H Collins

PMID: 15043264 DOI: 10.1016/j.chroma.2003.12.033

Abstract

A procedure is presented for the rapid evaluation of HPLC stationary phase stability at pH 8.4 or 10.1 using a temperature of 60 degrees C. Mobile phase (MeOH-0.1 mol l(-1) aqueous NaHCO3, 50:50, v/v) is continuously passed through the column with periodic injections of a test solution until the several chromatographic parameters of the resulting chromatograms are degraded. The tests were applied to several commercial and laboratory-made stationary phases. After degradation two of these phases, one commercial and one laboratory-made, were examined by elemental analysis and scanning electron microscopy to elucidate the degradation process.

MeSH terms

• Chromatography, High Pressure Liquid / instrumentation
• Hydrogen-Ion Concentration

Publication Types

• Evaluation Study
• Journal Article
• Research Support, Non-U.S. Gov't