Plant Physiol. 1981 Oct;68(4):964-8. doi: 10.1104/pp.68.4.964.
Plant physiology
M A Smith
PMID: 16662034 PMCID: PMC426021 DOI: 10.1104/pp.68.4.964
Antiserum was raised against a synthetic nona-peptide which was predicted to have considerable homology with the unhydroxylated, unglycosylated precursor of cell wall proteins from several plants. The antiserum is able to recognize the major cell wall protein of incubated carrot (Daucus carota) root discs which is produced when the discs are treated with a proline hydroxylase inhibitor and then labeled with radioactive proline. This technique has potential applications in studying cell wall biosynthesis and its regulatory control mechanisms.