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McNiven MA, Gallant RK, Richardson GF. In vitro methods of assessing the viability of rainbow trout spermatozoa. Theriogenology. 1992;38(4):679-86doi: 10.1016/0093-691x(92)90030-u.
McNiven, M. A., Gallant, R. K., & Richardson, G. F. (1992). In vitro methods of assessing the viability of rainbow trout spermatozoa. Theriogenology, 38(4), 679-86. https://doi.org/10.1016/0093-691x(92)90030-u
McNiven, M A, et al. "In vitro methods of assessing the viability of rainbow trout spermatozoa." Theriogenology vol. 38,4 (1992): 679-86. doi: https://doi.org/10.1016/0093-691x(92)90030-u
McNiven MA, Gallant RK, Richardson GF. In vitro methods of assessing the viability of rainbow trout spermatozoa. Theriogenology. 1992 Oct;38(4):679-86. doi: 10.1016/0093-691x(92)90030-u. PMID: 16727170.
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Theriogenology. 1992 Oct;38(4):679-86. doi: 10.1016/0093-691x(92)90030-u.

In vitro methods of assessing the viability of rainbow trout spermatozoa.

Theriogenology

M A McNiven, R K Gallant, G F Richardson

Affiliations

  1. Department of Health Management, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, P.E.I. C1A 4P3 Canada.

PMID: 16727170 DOI: 10.1016/0093-691x(92)90030-u

Abstract

The accuracy of three in vitro methods for estimating the proportion of dead rainbow trout (Onchorhynchus mykiss) spermatozoa was investigated. Motility rating, fluorometry using ethidium bromide, and lactate dehydrogenase (LDH) activity in seminal plasma were compared. Semen samples were prepared to contain 0, 25, 50, 75 and 100% killed spermatozoa. All three methods demonstrated highly significant relationships (P<0.001) with the percentage of killed spermatozoa. Motility rating was found to be quick and accurate but required experienced workers and the results thus could vary between evaluators. Fluorometry was rapid and relatively simple to perform and required only a small amount of semen. Measurement of LDH activity in seminal plasma was accurate but time-consuming and required large amounts of semen.

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