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Gallant RK, Richardson GF, McNiven MA. Comparison of different extenders for the cryopreservation of Atlantic salmon spermatozoa. Theriogenology. 1993;40(3):479-86doi: 10.1016/0093-691x(93)90401-p.
Gallant, R. K., Richardson, G. F., & McNiven, M. A. (1993). Comparison of different extenders for the cryopreservation of Atlantic salmon spermatozoa. Theriogenology, 40(3), 479-86. https://doi.org/10.1016/0093-691x(93)90401-p
Gallant, R K, et al. "Comparison of different extenders for the cryopreservation of Atlantic salmon spermatozoa." Theriogenology vol. 40,3 (1993): 479-86. doi: https://doi.org/10.1016/0093-691x(93)90401-p
Gallant RK, Richardson GF, McNiven MA. Comparison of different extenders for the cryopreservation of Atlantic salmon spermatozoa. Theriogenology. 1993 Sep;40(3):479-86. doi: 10.1016/0093-691x(93)90401-p. PMID: 16727331.
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Theriogenology. 1993 Sep;40(3):479-86. doi: 10.1016/0093-691x(93)90401-p.

Comparison of different extenders for the cryopreservation of Atlantic salmon spermatozoa.

Theriogenology

R K Gallant, G F Richardson, M A McNiven

Affiliations

  1. Department of Health Management, Atlantic Veterinary College, University of Prince Edward Island, 550 University Avenue, Charlottetown, PEI, Canada.

PMID: 16727331 DOI: 10.1016/0093-691x(93)90401-p

Abstract

Fifteen extenders were produced by adding dimethyl sulfoxide (DMSO) at 8, 10 or 12% of diluent volume to 5 diluents. All extenders were cooled to 4 degrees C. Pooled Atlantic salmon (Salmo salar) semen with greater than 90% progressive motility was kept at 4 degrees C and added to each extender so that the semen was diluted 1:3 (semen:extender). The equilibration time was less than 5 minutes at 4 degrees C. The extended semen was loaded into 0.5-ml straws and was cooled from 4 degrees C to -90 degrees C at a rate of 30 degrees C per minute. The straws were then plunged into liquid nitrogen for storage. Fluorometry was used to determine the viability of the semen in each of the extenders after freezing and thawing. Cryopreservation of Atlantic salmon semen in Extender 3 (0.137 M NaCl, 0.011 M KCl, 0.004 M Na(2)HPO(4).7H(2)O, 7.5 g/l L-alpha-lecithin and 12% dimethyl sulfoxide) and Extender 12 (0.100 M KHCO(3), 0.0065 M reduced glutathione, 0.125 M sucrose and 12% dimethyl sulfoxide) resulted in significantly (P<0.05) lower percentages of dead spermatozoa than for the other extenders. Furthermore, there was a significantly (P<0.05) lower percentage of dead cells in Extender 3 than in Extender 12.

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