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Suzuki T, Matsuzaki T, Hagiwara H, et al. Apical localization of sodium-dependent glucose transporter SGLT1 is maintained by cholesterol and microtubules. Acta Histochem Cytochem. 2006;39(6):155-61doi: 10.1267/ahc.06024.
Suzuki, T., Matsuzaki, T., Hagiwara, H., Aoki, T., Tajika-Takahashi, Y., & Takata, K. (2006). Apical localization of sodium-dependent glucose transporter SGLT1 is maintained by cholesterol and microtubules. Acta histochemica et cytochemica, 39(6), 155-61. https://doi.org/10.1267/ahc.06024
Suzuki, Takeshi, et al. "Apical localization of sodium-dependent glucose transporter SGLT1 is maintained by cholesterol and microtubules." Acta histochemica et cytochemica vol. 39,6 (2006): 155-61. doi: https://doi.org/10.1267/ahc.06024
Suzuki T, Matsuzaki T, Hagiwara H, Aoki T, Tajika-Takahashi Y, Takata K. Apical localization of sodium-dependent glucose transporter SGLT1 is maintained by cholesterol and microtubules. Acta Histochem Cytochem. 2006 Dec 28;39(6):155-61. doi: 10.1267/ahc.06024. Epub 2006 Dec 06. PMID: 17327902; PMCID: PMC1779948.
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Acta Histochem Cytochem. 2006 Dec 28;39(6):155-61. doi: 10.1267/ahc.06024. Epub 2006 Dec 06.

Apical localization of sodium-dependent glucose transporter SGLT1 is maintained by cholesterol and microtubules.

Acta histochemica et cytochemica

Takeshi Suzuki, Toshiyuki Matsuzaki, Haruo Hagiwara, Takeo Aoki, Yukiko Tajika-Takahashi, Kuniaki Takata

Affiliations

  1. Department of Anatomy and Cell Biology, Gunma University Graduate School of Medicine, Maebashi, Gunma 371-8511, Japan.

PMID: 17327902 PMCID: PMC1779948 DOI: 10.1267/ahc.06024

Abstract

A GFP-labeled sodium-dependent glucose transporter SGLT1 (SGLT-GFP) was transfected into MDCK cells. SGLT-GFP was localized at the apical membrane in confluent cells. When cellular cholesterol was depleted by methyl-beta-cyclodextrin (MbetaCD) treatment, the localization of SGLT-GFP gradually switched from apical to whole plasma membrane. Time-lapse microscopy revealed that the effect of MbetaCD appeared within 30 min, and that the transition of SGLT-GFP to the whole plasma membrane was completed within 2 hr after the administration. Immunofluorescence microscopy revealed that the tight junction framework remained steady during this process. The effect of MbetaCD on SGLT-GFP localization was counter-balanced by the addition of cholesterol into the culture medium. Disruption of microtubules by colcemid also perturbed SGLT-GFP localization. SGLT-GFP localized to the whole plasma membrane by colcemid treatment, and apical localization was restored within 1 hr after -removal of colcemid. Inhibition of protein synthesis by cycloheximide had no effect on the transition of SGLT-GFP induced by the MbetaCD or colcemid. These results indicated that the apical localization of SGLT-GFP is maintained by cellular cholesterol and microtubules, possibly with an apical recycling machinery.

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