Se Pu. 2008 Jan;26(1):15-21.

[Application of two-dimensional liquid chromatography coupled with mass spectrometry for the separation of active compounds in Gegen Qinlian decoction].

Se pu = Chinese journal of chromatography

[Article in Chinese]
Fei Guo, Yan Wang, Renfeng Wang, Chao Yan

PMID: 18438018

Abstract

An system of two-dimensional liquid chromatography (2D-LC) coupled with mass spectrometry was developed for the separation of components in Gegen Qinlian decoction, a complex traditional Chinese medicine. A CN column was used in the first dimensional chromatography, with gradient elution using water and methanol as the mobile phase A and mobile phase B, respectively; an ODS column was used in the second dimensional chromatography, with gradient elution using 20 mmol/L ammonium acetate buffer and acetonitrile as the mobile phase A and mobile phase B, respectively. The effluent was detected by mass spectrometry with electrospray ionization (ESI)/atmospheric pressure ionization (APCI) combined ion source and positive and negative scan modes. The components in Gegen Qinlian decoction was separated with the 2D-LC system with peak capacity up to 8000. Compared with UV detection, MS detected more components, especially in the negative mode. The 2D-LC/MS system provides higher peak capacity, better resolution and the capacity of peak identification. It is suitable for the analysis of complex samples such as the traditional Chinese medicine.

Substances

• Drugs, Chinese Herbal
• gegenqinlian

MeSH terms

• Chromatography, Liquid / methods
• Drugs, Chinese Herbal / chemistry
• Drugs, Chinese Herbal / isolation & purification
• Mass Spectrometry / methods

Publication Types

• English Abstract
• Journal Article