Methods Mol Biol. 2008;453:105-22. doi: 10.1007/978-1-60327-429-6_4.
Methods in molecular biology (Clifton, N.J.)
Rune Matthiesen, Ole N Jensen
PMID: 18712299 DOI: 10.1007/978-1-60327-429-6_4
The systematic study of proteins and protein networks, that is, proteomics, calls for qualitative and quantitative analysis of proteins and peptides. Mass spectrometry (MS) is a key analytical technology in current proteomics and modern mass spectrometers generate large amounts of high-quality data that in turn allow protein identification, annotation of secondary modifications, and determination of the absolute or relative abundance of individual proteins. Advances in mass spectrometry-driven proteomics rely on robust bioinformatics tools that enable large-scale data analysis. This chapter describes some of the basic concepts and current approaches to the analysis of MS and MS/MS data in proteomics.
