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Lee JY, Roh JR, Kim HS. Metabolic engineering of Pseudomonas putida for the simultaneous biodegradation of benzene, toluene, and p-xylene mixture. Biotechnol Bioeng. 1994;43(11):1146-52doi: 10.1002/bit.260431120.
Lee, J. Y., Roh, J. R., & Kim, H. S. (1994). Metabolic engineering of Pseudomonas putida for the simultaneous biodegradation of benzene, toluene, and p-xylene mixture. Biotechnology and bioengineering, 43(11), 1146-52. https://doi.org/10.1002/bit.260431120
Lee, J Y, et al. "Metabolic engineering of Pseudomonas putida for the simultaneous biodegradation of benzene, toluene, and p-xylene mixture." Biotechnology and bioengineering vol. 43,11 (1994): 1146-52. doi: https://doi.org/10.1002/bit.260431120
Lee JY, Roh JR, Kim HS. Metabolic engineering of Pseudomonas putida for the simultaneous biodegradation of benzene, toluene, and p-xylene mixture. Biotechnol Bioeng. 1994 May;43(11):1146-52. doi: 10.1002/bit.260431120. PMID: 18615528.
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Biotechnol Bioeng. 1994 May;43(11):1146-52. doi: 10.1002/bit.260431120.

Metabolic engineering of Pseudomonas putida for the simultaneous biodegradation of benzene, toluene, and p-xylene mixture.

Biotechnology and bioengineering

J Y Lee, J R Roh, H S Kim

Affiliations

  1. Department of Biotechnology, Korea Advanced Institute of Science and Technology, 373-1, Kusung-Dong, Yusung-Gu, Taejon 305-701, Korea.

PMID: 18615528 DOI: 10.1002/bit.260431120

Abstract

For the complete biodegradation of a mixture of benzene, toluene, and p-xylene (BTX), a critical metabolic step that can connect two existing metabolic pathways of aromatic compounds (the tod and the tol pathways) was determined. Toluate-cis-glycol dehydrogenase in the tol pathway was found to attack benzene-cis-glycol, toluene-cis-glycol, and p-xylene-cis-glycol, which are metabolic intermediates of the tod pathway. Based on this observation, a hybrid strain, Pseudomonase putida TB101, was constructed by introduction of the TOL plasmid pWW0 into P. putida F39/D, a derivative of P. putida F1, which is unable to transform cis-glycol compounds to corresponding catechols. The metabolic flux of BTX into the tod pathway was redirected to the tol pathway at the level of cis-glycol compounds by the action of toluate-cis-glycol dehydrogenase in P. putida TB101, resulting in the simultaneous mineralization of BTX mixture without accumulation of any metabolic intermediates. The profile of specific degradation rates showed a similar pattern as that of the specific growth rate of the microorganism, and the maximum specific degradation rates of benzene, toluene, and p-xylene were determined to be about 0.27, 0.86, and 2.89 mg/mg biomass/h, respectively. P. putida TB101 is the first reported microorganism that mineralizes BTX mixture simultaneously. (c) 1994 John Wiley & Sons, Inc.

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