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Xu Q, Zhao Z, Ni Y, et al. Effect of recombinant growth hormone on expression of growth hormone receptor, insulin-like growth factor mRNA and serum level of leptin in growing pigs. Sci China C Life Sci. 2003;46(2):205-11doi: 10.1360/03yc9022.
Xu, Q., Zhao, Z., Ni, Y., Zhao, R., & Chen, J. (2003). Effect of recombinant growth hormone on expression of growth hormone receptor, insulin-like growth factor mRNA and serum level of leptin in growing pigs. Science in China. Series C, Life sciences, 46(2), 205-11. https://doi.org/10.1360/03yc9022
Xu, Qingfu, et al. "Effect of recombinant growth hormone on expression of growth hormone receptor, insulin-like growth factor mRNA and serum level of leptin in growing pigs." Science in China. Series C, Life sciences vol. 46,2 (2003): 205-11. doi: https://doi.org/10.1360/03yc9022
Xu Q, Zhao Z, Ni Y, Zhao R, Chen J. Effect of recombinant growth hormone on expression of growth hormone receptor, insulin-like growth factor mRNA and serum level of leptin in growing pigs. Sci China C Life Sci. 2003 Apr;46(2):205-11. doi: 10.1360/03yc9022. PMID: 18758711.
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Sci China C Life Sci. 2003 Apr;46(2):205-11. doi: 10.1360/03yc9022.

Effect of recombinant growth hormone on expression of growth hormone receptor, insulin-like growth factor mRNA and serum level of leptin in growing pigs.

Science in China. Series C, Life sciences

Qingfu Xu, Zhihui Zhao, Yingdong Ni, Ruqian Zhao, Jie Chen

Affiliations

  1. Laboratory of Animal Physiology & Biochemistry, College of Animal Medicine, Nanjing Agricultural University, 210095, Nanjing, China.

PMID: 18758711 DOI: 10.1360/03yc9022

Abstract

Sixteen Large White x Landrace castrated male pigs were allotted into treatment and control group. The treatment group was injected intramuscularly with recombinant porcine growth hormone (rpGH, 4 mg d(-1)) and the control group with vehicle for 28 days. Animals were slaughtered 4 h after final injection for liver, longissimus dorsi (LD) muscle and blood sampling. Serum concentration of insulin-like growth factor 1 (IGF-I) and leptin were determined by RIA. The total RNA was extracted from tissues to measure the abundance of growth hormone receptor (GHR), IGF-I mRNA by RT-PCR with 18S rRNA internal standard. Results showed that rpGH enhanced the average daily weight gain by 26.1% (P < 0.05), the serum IGF-I concentration by 70.94% (P < 0.01), decreased serum leptin by 34.8% (P < 0.01). The relative abundance of GHR and IGF-I mRNA in liver were increased by 24.45% (P < 0.05) and 45.30% (P < 0.01), respectively, but no difference of GHR (P > 0.05) and IGF-I mRNA (P > 0.05) in LD between GH treated and control group was found. These results suggest that rpGH can up-regulate hepatic GHR and IGF-I gene expression and improve animal growth. However the effect of rpGH on GHR and IGF-I gene expression are tissue-specific.

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