Display options
Cite
Chen J, Ni H, Sun J, et al. G protein beta(1)gamma (2) subunits purification and their interaction with adenylyl cyclase. Sci China C Life Sci. 2003;46(2):212-23doi: 10.1360/03yc9023.
Chen, J., Ni, H., Sun, J., & Weng, G. (2003). G protein beta(1)gamma (2) subunits purification and their interaction with adenylyl cyclase. Science in China. Series C, Life sciences, 46(2), 212-23. https://doi.org/10.1360/03yc9023
Chen, Julian, et al. "G protein beta(1)gamma (2) subunits purification and their interaction with adenylyl cyclase." Science in China. Series C, Life sciences vol. 46,2 (2003): 212-23. doi: https://doi.org/10.1360/03yc9023
Chen J, Ni H, Sun J, Weng G. G protein beta(1)gamma (2) subunits purification and their interaction with adenylyl cyclase. Sci China C Life Sci. 2003 Apr;46(2):212-23. doi: 10.1360/03yc9023. PMID: 18758712.
Copy Download .nbib Format: NLM
Share it on

Sci China C Life Sci. 2003 Apr;46(2):212-23. doi: 10.1360/03yc9023.

G protein beta(1)gamma (2) subunits purification and their interaction with adenylyl cyclase.

Science in China. Series C, Life sciences

Julian Chen, Hanxiang Ni, Jingrui Sun, Gezhi Weng

Affiliations

  1. Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 100094, Beijing, China, [email protected].

PMID: 18758712 DOI: 10.1360/03yc9023

Abstract

A preliminary study on the interaction of G protein (guanine triphosphate binding protein) beta(1)gamma(2) subunits and their coupled components in cell signal transduction was conducted in vitro. The insect cell lines, Sf9 (Spodoptera frugiperda) and H5 (Trichoplusia ni) were used to express the recombinant protein Gbeta(1)gamma(2). The cell membrane containing Gbeta(1)gamma(2) was isolated through affinity chromatography column with Ni-NTAagarose by FPLC method, and the highly purified protein was obtained. The adenylyl cyclase 2 (AC(2)) activity assay showed that the purified Gbeta(1)gamma(2) could significantly stimulate AC(2) activity. The interaction of beta(1)gamma(2) subunits of G protein with the cytoplasmic tail of various mammalian adenylyl cyclases was monitored by BIAcore technology using NTA sensor chip, which relies on the phenomenon of surface plasmon resonance (SPR). The experiments showed the direct binding of Gbeta(1)gamma(2) to the cytoplasmic tail C2 domain of AC2. The specific binding domain of AC2 with Gbeta(1)gamma(2) was the same as AC2 activity domain which was stimulated by Gbeta(1)gamma(2).

Publication Types