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Su AK, Chang YS, Lin CH. Analysis of riboflavin in beer by capillary electrophoresis/blue light emitting diode (LED)-induced fluorescence detection combined with a dynamic pH junction technique. Talanta. 2004;64(4):970-4doi: 10.1016/j.talanta.2004.04.011.
Su, A. K., Chang, Y. S., & Lin, C. H. (2004). Analysis of riboflavin in beer by capillary electrophoresis/blue light emitting diode (LED)-induced fluorescence detection combined with a dynamic pH junction technique. Talanta, 64(4), 970-4. https://doi.org/10.1016/j.talanta.2004.04.011
Su, An-Kai, et al. "Analysis of riboflavin in beer by capillary electrophoresis/blue light emitting diode (LED)-induced fluorescence detection combined with a dynamic pH junction technique." Talanta vol. 64,4 (2004): 970-4. doi: https://doi.org/10.1016/j.talanta.2004.04.011
Su AK, Chang YS, Lin CH. Analysis of riboflavin in beer by capillary electrophoresis/blue light emitting diode (LED)-induced fluorescence detection combined with a dynamic pH junction technique. Talanta. 2004 Nov 15;64(4):970-4. doi: 10.1016/j.talanta.2004.04.011. PMID: 18969698.
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Talanta. 2004 Nov 15;64(4):970-4. doi: 10.1016/j.talanta.2004.04.011.

Analysis of riboflavin in beer by capillary electrophoresis/blue light emitting diode (LED)-induced fluorescence detection combined with a dynamic pH junction technique.

Talanta

An-Kai Su, Yen-Sheng Chang, Cheng-Huang Lin

Affiliations

  1. Department of Chemistry, National Taiwan Normal University, 88 Sec. 4, Tingchow Road, Taipei, Taiwan.

PMID: 18969698 DOI: 10.1016/j.talanta.2004.04.011

Abstract

A simple, inexpensive and reliable method for the routine analysis of riboflavin in beer by capillary electrophoresis-light emitting diode (CE-LED) induced fluorescence detection is described. A simple and straightforward sample preparation is involved and the method is based on an inexpensive blue LED as the light source combined with an on-line sample concentration technique. For this detection system, using a normal micellar electrokinetic chromatography (MEKC), stacking-MEKC and dynamic pH junction techniques, the detection limits were found to be 480, 20 and 1ngmL(-1), respectively. In addition, the number of theoretical plates for riboflavin was determined to be 3.8x10(4) by means of a dynamic pH junction and this was improved to 3.2x10(6) when the dynamic pH junction-sweeping mode was applied. The concentrations of riboflavin in 12 samples of different types of commercial beer were found to be in the range of 130-280ngmL(-1).

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