Display options
Cite
Kinkel AD, Fernyhough ME, Helterline DL, et al. Oil red-O stains non-adipogenic cells: a precautionary note. Cytotechnology. 2005;46(1):49-56doi: 10.1007/s10616-004-3903-4.
Kinkel, A. D., Fernyhough, M. E., Helterline, D. L., Vierck, J. L., Oberg, K. S., Vance, T. J., Hausman, G. J., Hill, R. A., & Dodson, M. V. (2004). Oil red-O stains non-adipogenic cells: a precautionary note. Cytotechnology, 46(1), 49-56. https://doi.org/10.1007/s10616-004-3903-4
Kinkel, Adrienne D, et al. "Oil red-O stains non-adipogenic cells: a precautionary note." Cytotechnology vol. 46,1 (2004): 49-56. doi: https://doi.org/10.1007/s10616-004-3903-4
Kinkel AD, Fernyhough ME, Helterline DL, Vierck JL, Oberg KS, Vance TJ, Hausman GJ, Hill RA, Dodson MV. Oil red-O stains non-adipogenic cells: a precautionary note. Cytotechnology. 2004 Sep;46(1):49-56. doi: 10.1007/s10616-004-3903-4. Epub 2005 Jun 16. PMID: 19003258; PMCID: PMC3449473.
Copy Download .nbib Format: NLM
Share it on

Cytotechnology. 2004 Sep;46(1):49-56. doi: 10.1007/s10616-004-3903-4. Epub 2005 Jun 16.

Oil red-O stains non-adipogenic cells: a precautionary note.

Cytotechnology

Adrienne D Kinkel, Melinda E Fernyhough, Deri L Helterline, Janet L Vierck, Karen S Oberg, Tyler J Vance, Gary J Hausman, Rodney A Hill, Michael V Dodson

Affiliations

  1. Muscle Biology Laboratory, Department of Animal Sciences , Washington State University, Pullman, WA, 99164, USA.

PMID: 19003258 PMCID: PMC3449473 DOI: 10.1007/s10616-004-3903-4

Abstract

Bovine adipofibroblasts, 3T3-L1 cells, L-6 myogenic cells, and sheep satellite cells were allowed to proliferate for 48 h. Oil red-O (ORO) was dissolved in three different solvents isopropanol, propylene glycol and triethyl phosphate. At 48 h, the proliferative cultures were stained with the three stains. ORO stain prepared in both propylene glycol and triethyl phosphate resulted in bright red droplets appearing in all cultures, whereas ORO dissolved in isopropanol was not taken up by any of the cells. These data suggest that certain preparations of ORO may stain cells in non-adipogenic lineages as well as undifferentiated pre-adipocytes. Caution must be exercised when choosing solvents for ORO in differentiation studies using cells of the fat/adipose lineage.

References

  1. Yale J Biol Med. 1975 Mar;48(1):9-16 - PubMed
  2. Methods Mol Biol. 1990;5:197-207 - PubMed
  3. Histochem Cell Biol. 2002 Nov;118(5):423-8 - PubMed
  4. Histochem Cell Biol. 2001 Jul;116(1):63-8 - PubMed
  5. Med Lab Technol. 1974 Oct;31(4):335-41 - PubMed
  6. Histochemistry. 1992 Jul;97(6):493-7 - PubMed

Publication Types