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Showing 1 to 12 of 2464 entries
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Culture conditions for bovine embryonic stem cell-like cells isolated from blastocysts after external fertilization.

Cytotechnology

Jin M, Wu A, Dorzhin S, Yue Q, Ma Y, Liu D.
PMID: 22438181
Cytotechnology. 2012 Aug;64(4):379-89. doi: 10.1007/s10616-011-9408-z. Epub 2012 Mar 22.

Although isolation and characterization of embryonic stem cells have been successful in cattle, maintenance of bovine embryonic stem cells in culture remains difficult. In this study, we compared different methods of cell passaging, feeder cell layers and medium conditions...

The effect of medium selection on adipose-derived stem cell expansion and differentiation: implications for application in regenerative medicine.

Cytotechnology

Roxburgh J, Metcalfe AD, Martin YH.
PMID: 25795468
Cytotechnology. 2016 Aug;68(4):957-67. doi: 10.1007/s10616-015-9848-y. Epub 2015 Mar 21.

The use of adipose-derived stem cells is wide-spread in both basic biology and regenerative medicine, due to the abundance of adipose tissue and the multipotent differentiation potential of the cells. However, the methods used to isolate and culture cells...

Model-based strategy for cell culture seed train layout verified at lab scale.

Cytotechnology

Kern S, Platas-Barradas O, Pörtner R, Frahm B.
PMID: 25795469
Cytotechnology. 2016 Aug;68(4):1019-32. doi: 10.1007/s10616-015-9858-9. Epub 2015 Mar 21.

Cell culture seed trains-the generation of a sufficient viable cell number for the inoculation of the production scale bioreactor, starting from incubator scale-are time- and cost-intensive. Accordingly, a seed train offers potential for optimization regarding its layout and the...

Glioma targeting peptide in combination with the P53 C terminus inhibits glioma cell proliferation in vitro.

Cytotechnology

Wang D, Guo M, Yu J, Wang X, Zhang Q, Yang X, Li J, Zhao C, Feng B.
PMID: 28879517
Cytotechnology. 2018 Feb;70(1):153-161. doi: 10.1007/s10616-017-0122-3. Epub 2017 Sep 06.

Glioma is a prevalent malignant primary brain tumor in adults, the treatment for which remains a challenge due to its high infiltration and recurrence. Hence, treatments that lead to the suppression of glioma cell migration and invasion may be...

The effect of a number of H9C2 rat cardiomyocytes passage on repeatability of cytotoxicity study results.

Cytotechnology

Witek P, Korga A, Burdan F, Ostrowska M, Nosowska B, Iwan M, Dudka J.
PMID: 26946144
Cytotechnology. 2016 Dec;68(6):2407-2415. doi: 10.1007/s10616-016-9957-2. Epub 2016 Mar 05.

The embryonic cardiomyocyte cell line H9C2 is commonly used in numerous in vitro studies, including cardiotoxicity analyses of new drugs. So far no results were published for studies on cell parameters variability during the cell line ageing process. For...

Antidiabetic effect of enterolactone in cultured muscle cells and in type 2 diabetic model db/db mice.

Cytotechnology

Zhou F, Furuhashi K, Son MJ, Toyozaki M, Yoshizawa F, Miura Y, Yagasaki K.
PMID: 27000262
Cytotechnology. 2017 Jun;69(3):493-502. doi: 10.1007/s10616-016-9965-2. Epub 2016 Mar 21.

Enterolactone (ENL) is formed by the conversion of dietary precursors like strawberry lignans via the gut microbiota. Urinary concentrations of lignan metabolites are reported to be significantly associated with a lower risk of Type 2 diabetes (T2D). In the...

Comparative studies of cellular viability levels on 2D and 3D in vitro culture matrices.

Cytotechnology

Gargotti M, Lopez-Gonzalez U, Byrne HJ, Casey A.
PMID: 28924965
Cytotechnology. 2018 Feb;70(1):261-273. doi: 10.1007/s10616-017-0139-7. Epub 2017 Sep 18.

In this study, the cellular viability and function of immortalized human cervical and dermal cells are monitored and compared in conventional 2D and two commercial 3D membranes, Collagen and Geltrex, of varying working concentration and volume. Viability was monitored...

Seleno-short-chain chitosan induces apoptosis in human non-small-cell lung cancer A549 cells through ROS-mediated mitochondrial pathway.

Cytotechnology

Zhao Y, Zhang S, Wang P, Fu S, Wu D, Liu A.
PMID: 28421411
Cytotechnology. 2017 Dec;69(6):851-863. doi: 10.1007/s10616-017-0098-z. Epub 2017 Apr 18.

Seleno-short-chain chitosan (SSCC) is a synthesized chitosan derivative. In this study, antitumor activity and underlying mechanism of SSCC on human non-small-cell lung cancer A549 cells were investigated in vitro. The MTT assay showed that SSCC could inhibit cell viability...

Effect of linear alkylbenzene sulfonate (LAS) on human intestinal Caco-2 cells at non cytotoxic concentrations.

Cytotechnology

Bradai M, Han J, Omri AE, Funamizu N, Sayadi S, Isoda H.
PMID: 25999174
Cytotechnology. 2016 Aug;68(4):1267-75. doi: 10.1007/s10616-015-9887-4. Epub 2015 May 22.

Linear alkylbenzene sulfonate (LAS) is a cytotoxic synthetic anionic surfactant widely present in the environment due to its large-scale production and intensive use in the detergency field. In this study, we investigated the effect of LAS (CAS No. 25155-30-0)...

Using simple models to describe the kinetics of growth, glucose consumption, and monoclonal antibody formation in naive and infliximab producer CHO cells.

Cytotechnology

López-Meza J, Araíz-Hernández D, Carrillo-Cocom LM, López-Pacheco F, Rocha-Pizaña Mdel R, Alvarez MM.
PMID: 26091615
Cytotechnology. 2016 Aug;68(4):1287-300. doi: 10.1007/s10616-015-9889-2. Epub 2015 Jun 20.

Despite their practical and commercial relevance, there are few reports on the kinetics of growth and production of Chinese hamster ovary (CHO) cells-the most frequently used host for the industrial production of therapeutic proteins. We characterize the kinetics of...

Celastrus paniculatus Willd. mitigates t-BHP induced oxidative and apoptotic damage in C2C12 murine muscle cells.

Cytotechnology

Kumar KH, Venuprasad MP, Jayashree GV, Rachitha P, Krupashree K, Pal A, Khanum F.
PMID: 25118832
Cytotechnology. 2015 Dec;67(6):955-67. doi: 10.1007/s10616-014-9733-0. Epub 2014 Aug 15.

Identification, exploration and scientific validation of antioxidant rich herbal extracts to mitigate the radical induced cell damage provide new insights in the field of ayurvedic research/therapies. In the present study, we evaluated the anti-oxidant and anti-apoptotic potential of Celastrus...

Analyses of basal media and serum for in vitro expansion of suspension peripheral blood mononucleated stem cell.

Cytotechnology

Zainal Ariffin SH, Mohamed Rozali NA, Megat Abdul Wahab R, Senafi S, Zainol Abidin IZ, Zainal Ariffin Z.
PMID: 26231833
Cytotechnology. 2016 Aug;68(4):675-86. doi: 10.1007/s10616-014-9819-8. Epub 2015 Aug 01.

Transplantation of stem cells requires a huge amount of cells, deeming the expansion of the cells in vitro necessary. The aim of this study is to define the optimal combination of basal medium and serum for the expansion of...

Showing 1 to 12 of 2464 entries