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Kern S, Platas-Barradas O, Pörtner R, et al. Model-based strategy for cell culture seed train layout verified at lab scale. Cytotechnology. 2015;68(4):1019-32doi: 10.1007/s10616-015-9858-9.
Kern, S., Platas-Barradas, O., Pörtner, R., & Frahm, B. (2016). Model-based strategy for cell culture seed train layout verified at lab scale. Cytotechnology, 68(4), 1019-32. https://doi.org/10.1007/s10616-015-9858-9
Kern, Simon, et al. "Model-based strategy for cell culture seed train layout verified at lab scale." Cytotechnology vol. 68,4 (2016): 1019-32. doi: https://doi.org/10.1007/s10616-015-9858-9
Kern S, Platas-Barradas O, Pörtner R, Frahm B. Model-based strategy for cell culture seed train layout verified at lab scale. Cytotechnology. 2016 Aug;68(4):1019-32. doi: 10.1007/s10616-015-9858-9. Epub 2015 Mar 21. PMID: 25795469; PMCID: PMC4960151.
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Cytotechnology. 2016 Aug;68(4):1019-32. doi: 10.1007/s10616-015-9858-9. Epub 2015 Mar 21.

Model-based strategy for cell culture seed train layout verified at lab scale.

Cytotechnology

Simon Kern, Oscar Platas-Barradas, Ralf Pörtner, Björn Frahm

Affiliations

  1. Biotechnology and Bioprocess Engineering, Faculty of Life Science Technologies, Ostwestfalen-Lippe University of Applied Sciences, Liebigstr. 87, 32657, Lemgo, Germany.
  2. Institute of Bioprocess and Biosystems Engineering, Hamburg University of Technology, Denickestr. 15, 21073, Hamburg, Germany.
  3. Biotechnology and Bioprocess Engineering, Faculty of Life Science Technologies, Ostwestfalen-Lippe University of Applied Sciences, Liebigstr. 87, 32657, Lemgo, Germany. [email protected].

PMID: 25795469 PMCID: PMC4960151 DOI: 10.1007/s10616-015-9858-9

Abstract

Cell culture seed trains-the generation of a sufficient viable cell number for the inoculation of the production scale bioreactor, starting from incubator scale-are time- and cost-intensive. Accordingly, a seed train offers potential for optimization regarding its layout and the corresponding proceedings. A tool has been developed to determine the optimal points in time for cell passaging from one scale into the next and it has been applied to two different cell lines at lab scale, AGE1.HN AAT and CHO-K1. For evaluation, experimental seed train realization has been evaluated in comparison to its layout. In case of the AGE1.HN AAT cell line, the results have also been compared to the formerly manually designed seed train. The tool provides the same seed train layout based on the data of only two batches.

Keywords: Design; Layout; Modeling; Optimization; Seed train; Space–Time–Yield; Suspension cell cultures

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