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Jin M, Wu A, Dorzhin S, et al. Culture conditions for bovine embryonic stem cell-like cells isolated from blastocysts after external fertilization. Cytotechnology. 2012;64(4):379-89doi: 10.1007/s10616-011-9408-z.
Jin, M., Wu, A., Dorzhin, S., Yue, Q., Ma, Y., & Liu, D. (2012). Culture conditions for bovine embryonic stem cell-like cells isolated from blastocysts after external fertilization. Cytotechnology, 64(4), 379-89. https://doi.org/10.1007/s10616-011-9408-z
Jin, Muzi, et al. "Culture conditions for bovine embryonic stem cell-like cells isolated from blastocysts after external fertilization." Cytotechnology vol. 64,4 (2012): 379-89. doi: https://doi.org/10.1007/s10616-011-9408-z
Jin M, Wu A, Dorzhin S, Yue Q, Ma Y, Liu D. Culture conditions for bovine embryonic stem cell-like cells isolated from blastocysts after external fertilization. Cytotechnology. 2012 Aug;64(4):379-89. doi: 10.1007/s10616-011-9408-z. Epub 2012 Mar 22. PMID: 22438181; PMCID: PMC3397107.
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Cytotechnology. 2012 Aug;64(4):379-89. doi: 10.1007/s10616-011-9408-z. Epub 2012 Mar 22.

Culture conditions for bovine embryonic stem cell-like cells isolated from blastocysts after external fertilization.

Cytotechnology

Muzi Jin, Asga Wu, Sergei Dorzhin, Qunhua Yue, Yuzhen Ma, Dongjun Liu

Affiliations

  1. Key Laboratory of Mammalian Reproductive Biology and Biotechnology Ministry of Education, Inner Mongolia University, Inner Mongolia, 010021, Hohhot, China.

PMID: 22438181 PMCID: PMC3397107 DOI: 10.1007/s10616-011-9408-z

Abstract

Although isolation and characterization of embryonic stem cells have been successful in cattle, maintenance of bovine embryonic stem cells in culture remains difficult. In this study, we compared different methods of cell passaging, feeder cell layers and medium conditions for bovine embryonic stem cell-like cells. We found that a murine embryonic fibroblast feeder layer is more suitable for embryonic stem cell-like cells than bovine embryonic fibroblasts. When murine embryonic fibroblasts were used, a mechanical method of passaging led to better cell growth than passaging by trypsin digestion. We also found that exogenous supplementation with leukemia inhibitory factor maintained the embryonic stem cell-like cells in an undifferentiated state, whereas addition of stem cell factor resulted in their differentiation. Our findings provide an experimental basis for the establishment of an effective culture system for bovine embryonic stem cells.

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