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Kelly HT, Hill E, Reid F, et al. Comparison of RAP-PCR analysis of gene expression in fresh and immortalised rat hepatocyte cell lines. Cytotechnology. 2000;34(1):159-63doi: 10.1023/A:1008159912634.
Kelly, H. T., Hill, E., Reid, F., Grant, M. H., & Macdonald, C. (2000). Comparison of RAP-PCR analysis of gene expression in fresh and immortalised rat hepatocyte cell lines. Cytotechnology, 34(1), 159-63. https://doi.org/10.1023/A:1008159912634
Kelly, H T, et al. "Comparison of RAP-PCR analysis of gene expression in fresh and immortalised rat hepatocyte cell lines." Cytotechnology vol. 34,1 (2000): 159-63. doi: https://doi.org/10.1023/A:1008159912634
Kelly HT, Hill E, Reid F, Grant MH, Macdonald C. Comparison of RAP-PCR analysis of gene expression in fresh and immortalised rat hepatocyte cell lines. Cytotechnology. 2000 Oct;34(1):159-63. doi: 10.1023/A:1008159912634. PMID: 19003390; PMCID: PMC3449737.
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Cytotechnology. 2000 Oct;34(1):159-63. doi: 10.1023/A:1008159912634.

Comparison of RAP-PCR analysis of gene expression in fresh and immortalised rat hepatocyte cell lines.

Cytotechnology

H T Kelly, E Hill, F Reid, M H Grant, C Macdonald

Affiliations

  1. Biological Sciences, University of Paisley, Paisley, UK.

PMID: 19003390 PMCID: PMC3449737 DOI: 10.1023/A:1008159912634

Abstract

Primary rat hepatocytes dedifferentiate rapidly losing theactivities of the drug metabolising enzymes involved in thedetoxification of xenobiotics in the liver. An alternativeapproach to using primary hepatocytes for toxicity testing isthe development of immortalised hepatocyte cell lines via thetransfection of primary hepatocytes with SV40 DNA. In order toassess the suitability of immortalised lines as an alternativeto primary cell cultures we have used RNA arbitrarily primedpolymerase chain reaction to compare gene expression inimmortalised rat hepatocyte cell lines with that in primary rathepatocytes. We have found that differences exist in the RNAtranscripts between fresh and immortalised hepatocyteshighlighting RNA arbitrarily primed polymerase chain reaction asa useful screening method for identifying immortalised lineswhich retain the most ;normal' phenotype in relation to theprimary cells from which they originated.

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