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Lubel JS, Herath CB, Velkoska E, et al. Angiotensin converting enzyme 2 (ACE2) activity in fetal calf serum: implications for cell culture research. Cytotechnology. 2009;58(3):119-26doi: 10.1007/s10616-009-9185-0.
Lubel, J. S., Herath, C. B., Velkoska, E., Casley, D. J., Burrell, L. M., & Angus, P. W. (2008). Angiotensin converting enzyme 2 (ACE2) activity in fetal calf serum: implications for cell culture research. Cytotechnology, 58(3), 119-26. https://doi.org/10.1007/s10616-009-9185-0
Lubel, J S, et al. "Angiotensin converting enzyme 2 (ACE2) activity in fetal calf serum: implications for cell culture research." Cytotechnology vol. 58,3 (2008): 119-26. doi: https://doi.org/10.1007/s10616-009-9185-0
Lubel JS, Herath CB, Velkoska E, Casley DJ, Burrell LM, Angus PW. Angiotensin converting enzyme 2 (ACE2) activity in fetal calf serum: implications for cell culture research. Cytotechnology. 2008 Nov;58(3):119-26. doi: 10.1007/s10616-009-9185-0. Epub 2009 Feb 18. PMID: 19224387; PMCID: PMC2652558.
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Cytotechnology. 2008 Nov;58(3):119-26. doi: 10.1007/s10616-009-9185-0. Epub 2009 Feb 18.

Angiotensin converting enzyme 2 (ACE2) activity in fetal calf serum: implications for cell culture research.

Cytotechnology

J S Lubel, C B Herath, E Velkoska, D J Casley, L M Burrell, P W Angus

Affiliations

  1. Department of Medicine, Austin and Northern Health, Repatriation Hospital, University of Melbourne, Waterdale Road, Heidelberg Heights, Melbourne, VIC, Australia, [email protected].

PMID: 19224387 PMCID: PMC2652558 DOI: 10.1007/s10616-009-9185-0

Abstract

Cell culture experiments often employ the use of culture media that contain fetal calf serum (FCS). The angiotensin peptides angiotensin II and angiotensin 1-7 have opposing effects with angiotensin converting enzyme 2 (ACE2) being the enzyme predominantly responsible for generating angiotensin 1-7 from angiotensin II. The effect of FCS on angiotensin peptides has not previously been described. We have shown that FCS has ACE2 enzyme activity capable of degrading angiotensin II and generating angiotensin 1-7. Researchers should be aware that FCS possesses ACE2 activity and that heat-treating FCS to 56 degrees C only partially inhibits this enzyme activity, whereas heat-treating to 70 degrees C completely abolishes ACE2 activity.

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