Display options
Cite
Reimel BA, Pan S, May DH, et al. Proteomics on Fixed Tissue Specimens - A Review. Curr Proteomics. 2009;6(1):63-69doi: 10.2174/157016409787847420.
Reimel, B. A., Pan, S., May, D. H., Shaffer, S. A., Goodlett, D. R., McIntosh, M. W., Yerian, L. M., Bronner, M. P., Chen, R., & Brentnall, T. A. (2009). Proteomics on Fixed Tissue Specimens - A Review. Current proteomics, 6(1), 63-69. https://doi.org/10.2174/157016409787847420
Reimel, Beth Ann, et al. "Proteomics on Fixed Tissue Specimens - A Review." Current proteomics vol. 6,1 (2009): 63-69. doi: https://doi.org/10.2174/157016409787847420
Reimel BA, Pan S, May DH, Shaffer SA, Goodlett DR, McIntosh MW, Yerian LM, Bronner MP, Chen R, Brentnall TA. Proteomics on Fixed Tissue Specimens - A Review. Curr Proteomics. 2009 Apr 01;6(1):63-69. doi: 10.2174/157016409787847420. PMID: 19829741; PMCID: PMC2760948.
Copy Download .nbib Format: NLM
Share it on

Curr Proteomics. 2009 Apr 01;6(1):63-69. doi: 10.2174/157016409787847420.

Proteomics on Fixed Tissue Specimens - A Review.

Current proteomics

Beth Ann Reimel, Sheng Pan, Damon H May, Scott A Shaffer, David R Goodlett, Martin W McIntosh, Lisa M Yerian, Mary P Bronner, Ru Chen, Teresa A Brentnall

Affiliations

  1. Departments of Gastroenterology and Surgery, University of Washington, Seattle, Washington 98195.

PMID: 19829741 PMCID: PMC2760948 DOI: 10.2174/157016409787847420

Abstract

The vast majority of clinical tissue samples are formalin-fixed and paraffin-preserved. This type of preservation has been considered an obstacle to protein extraction from these tissues. However, these are the very tissue samples that have associated patient histories, diagnoses and outcomes - ideal samples in the quest to translate bench research into clinical applications. Thus, until recently, these valuable specimens have been unavailable for proteomic analysis.Over the last decade, researchers have been exploring efficient methods to undo protein cross-linking caused by standard tissue fixatives and extract proteins from archived tissue specimens. These methods have been applied in different clinical proteomic studies. In this report, we attempt to review the development of these techniques, summarize the proteomic findings, and discuss the impact on future clinical proteomics.

References

  1. J Proteome Res. 2007 Mar;6(3):1038-47 - PubMed
  2. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
  3. J Histochem Cytochem. 2006 Jun;54(6):739-43 - PubMed
  4. Histochem J. 1988 Jun-Jul;20(6-7):341-6 - PubMed
  5. J Histochem Cytochem. 2005 Nov;53(11):1421-32 - PubMed
  6. Mol Cell Proteomics. 2005 Nov;4(11):1741-53 - PubMed
  7. J Proteome Res. 2007 Apr;6(4):1295-305 - PubMed
  8. J Histochem Cytochem. 1998 Mar;46(3):397-403 - PubMed
  9. Oncogene. 2007 Jan 4;26(1):65-76 - PubMed
  10. Proteomics. 2003 Apr;3(4):413-21 - PubMed
  11. Lab Invest. 2005 Nov;85(11):1405-15 - PubMed
  12. Lab Invest. 2004 Mar;84(3):300-6 - PubMed
  13. J Histochem Cytochem. 2005 Jan;53(1):13-21 - PubMed
  14. J Proteome Res. 2004 May-Jun;3(3):604-12 - PubMed
  15. J Histochem Cytochem. 2007 Jul;55(7):763-72 - PubMed
  16. Anal Biochem. 1981 Apr;112(2):195-203 - PubMed
  17. J Proteome Res. 2005 Nov-Dec;4(6):2404-11 - PubMed
  18. Proteomics. 2008 Sep;8(18):3702-14 - PubMed
  19. Biotechniques. 2005 Jun;Suppl:32-5 - PubMed
  20. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 - PubMed
  21. Clin Cancer Res. 2008 Feb 15;14(4):1002-14 - PubMed

Publication Types

Grant support